Advance Search
LIU Yongxia, LIU Huan, LIU Yan, ZHAO Ni. Role of automatic nucleic acid detection analysis system in detecting of virus in voluntary blood donors[J]. Journal of Clinical Medicine in Practice, 2020, 24(23): 113-115. DOI: 10.7619/jcmp.202023034
Citation: LIU Yongxia, LIU Huan, LIU Yan, ZHAO Ni. Role of automatic nucleic acid detection analysis system in detecting of virus in voluntary blood donors[J]. Journal of Clinical Medicine in Practice, 2020, 24(23): 113-115. DOI: 10.7619/jcmp.202023034
shu

Role of automatic nucleic acid detection analysis system in detecting of virus in voluntary blood donors

  • Department of Blood Transfusion, Affiliated Hospital of Yan'an University, Yan'an, Shaanxi, 716000

More Information
  • Received Date: September 10, 2020
  • Available Online: December 20, 2020
    Graphical Abstract
    • Abstract
  • Objective To observe the effect of automatic nucleic acid detection analysis system in detection of hepatitis B virus(HBV), hepatitis C virus(HCV)and human immunodeficiency virus(HIV)in blood samples of blood donors. Methods A total of 1 229 volunteer blood donors were selected, two copies of blood in each donor were collected, 1 copy was used for Enzyme Linked Immunosorbent Assay(ELISA)and another one was used for nucleic acid detection. Results The positive ratesof HBV by nucleic acid test and ELISA test were 2.03% and 0.98%, respectively, there was significant difference(P<0.05). The positive rates of nucleic acid test and ELISA in detecting HCV were 1.22%and 1.14%, there was no significant difference(P>0.05). The positive rates of nucleic acid test and ELISA in HIV detection were 0.24%and 1.06%, there was significant difference(P<0.05). Conclusion Automatic nucleic acid detection analysis system has higher accuracy rate in screening HBV, HCV and HIV in voluntary blood donors, nucleic acid detection and ELISA detection have their own advantages, and the combination of the two methods can improve the accuracy of virus detection.
    • FullText(HTML)
    • References (15)
  • 赵棉, 张力, 赵亚妮. HBV DNA定量与乙肝血清学标志物定量联合检测乙肝病毒感染的效果分析[J]. 实用临床医药杂志, 2018, 22(15): 37-40.
    杨松, 朱秋华, 羊柳. 乙肝病毒感染诊断中乙肝血清学标志物定量与HBV DNA定量联合检测的应用研究[J]. 临床检验杂志, 2019, 8(2): 49-50.
    康文玉, 田子颖, 李琼芬, 等. 云南省2006年和2014年1~29岁人群乙型肝炎血清流行病学调查[J]. 中华流行病学杂志, 2017, 38(4): 518-521.
    门守山, 吕连智, 陈元锋, 等. 血站核酸检测实施后献血者血液检测模式的研究[J]. 中国实验血液学杂志, 2017, 25(6): 1815-1819.
    何子毅, 陈庆恺, 陈少彬, 等. 四种血液安全筛查模式对经血传播病毒残余风险的评估[J]. 中国输血杂志, 2018, 11(2): 45-48.
    徐晓燕, 翟小琳, 吕涛, 等. 不同血液病患者EB病毒和人巨细胞病毒核酸检测阳性率与年龄的相关性研究[J]. 中华医院感染学杂志, 2017, 27(7): 1500-1503.
    张锋, 张琼, 林碧, 等. 血清学联合核酸检测在献血者血液筛查中的互补性[J]. 中国艾滋病性病, 2016, 22(3): 197-199.
    曹振环, 柳雅立, 鲁俊锋, 等. 干扰素联合核苷(酸)类似物治疗HBeAg阳性慢性乙型肝炎48周临床研究[J]. 传染病信息, 2016, 29(2): 77-80.
    陈雅红, 洪思让, 陈明春, 等. 2015年泉州市1~59岁人群乙型肝炎血清流行病学特征及影响因素[J]. 中国疫苗和免疫, 2017, 23(5): 512-516.
    陈珍珍, 赵越, 王占黎, 等. 自制胶体金试剂盒对布鲁氏菌病的诊断效果评价[J]. 现代预防医学, 2016, 43(15): 2802-2805.
    唐玥, 杨益, 孙走南, 等. 新兵(学员)预防接种20μg重组乙型肝炎疫苗(酿酒酵母)的免疫效果分析[J]. 中国免疫学杂志, 2017, 33(3): 356-359.
    郭兆富, 尹以靖, 王付能, 等. 德宏地区HBsAg阴性HBV/HCV/HIV核酸联检反应性献血者HBV感染特征研究[J]. 中国输血杂志, 2016, 29(2): 173-177.
    何培元, 侯志平, 王春青, 等. 河北汉族人群白细胞介素-17血清表达水平及基因多态性与乙型肝炎病毒感染的关系[J]. 实用医学杂志, 2017, 33(2): 227-230.
    李天驹, 秦波, 李朋. microRNA-206单核苷酸多态性与乙型肝炎病毒相关原发性肝癌关系的初步研究[J]. 第三军医大学学报, 2016, 38(16): 1843-1848.
    邢同京, 徐洪涛, 陆欣星, 等. 微小核糖核酸-548ah靶向组蛋白去乙酰化酶4对乙型肝炎病毒复制和表达的影响[J]. 中华传染病杂志, 2016, 34(5): 274-279.
    • Related Articles

  • Related Articles

    [1]XIANG Mingfei, LIU Yingying, YU Mingjie. Correlations of serum calcium, 25-hydroxyvitamin D and uric acid levels with benign paroxysmal positional vertigo[J]. Journal of Clinical Medicine in Practice, 2024, 28(19): 118-122. DOI: 10.7619/jcmp.20241444
    [2]ZHANG Chenchen, LIU Yanling. Values of the Acute Physiology and Chronic Health Evaluation Ⅱ score combined with serum glycated hemoglobin and lactate dehydrogenase in evaluating the severity and prognosis of patients with acute pancreatitis[J]. Journal of Clinical Medicine in Practice, 2024, 28(17): 62-67. DOI: 10.7619/jcmp.20240043
    [3]HAO Yuqing, SUN Yu, WANG Qiaogai. Changes of serum homocysteine, lactate dehydrogenase and interleukin-17 levels in acute leukemia and their significance[J]. Journal of Clinical Medicine in Practice, 2024, 28(15): 115-119. DOI: 10.7619/jcmp.20241033
    [4]WAN Jiangwei, CHENG Dongru, WANG Tao, GAO Ping. Value of serum lactate dehydrogenase, β2 microglobulin and ferritin combined with modified WHO-based Prognostic Scoring System in predicting poor prognosis of myelodysplastic syndrome[J]. Journal of Clinical Medicine in Practice, 2024, 28(5): 79-84. DOI: 10.7619/jcmp.20233107
    [5]LEI Xiaoming, XUE Mengzhou, XIA Bin, LI Jing, GUO Pingping. Correlations of serum 25-hydroxyvitamin D and adiponectin with cognitive function in patients with white matter lesions[J]. Journal of Clinical Medicine in Practice, 2022, 26(19): 33-37. DOI: 10.7619/jcmp.20221686
    [6]LU Zhenhui, GUO Xiaoming, LI Xinling, CAO Zhiyong, ZHU Lianha, ZHU Xiangyang. Effect of serum 25-hydroxyvitamin D3 level on cognitive function after stroke in elderly patients with first-episode cerebral infarction[J]. Journal of Clinical Medicine in Practice, 2021, 25(17): 61-66. DOI: 10.7619/jcmp.20211950
    [7]DU Kaixian, HU Huihui, LI Lin, DONG Yan, GUAN Jing, SONG Chunlan, BAI Yanmin, KONG Lina, JIA Tianming. Value of fibronectin, vascular endothelial growth factor and lactate dehydrogenas levels in cerebrospinal fluid in diagnosing bacterial meningitis and viral encephalitis in children[J]. Journal of Clinical Medicine in Practice, 2021, 25(11): 26-30. DOI: 10.7619/jcmp.20210272
    [8]ZHANG Cuijun, SHI Li, SHENG Yulu. Investigation on serum 25-hydroxy-vitamin D levels in 4 013 pregnant women in middle or late stages[J]. Journal of Clinical Medicine in Practice, 2020, 24(22): 120-123. DOI: 10.7619/jcmp.202022035
    [9]LIU Xiaoxia, GONG Nini. Changes of serum 25-hydroxyvitamin D in patients with hypertension during pregnancy and its clinical significance[J]. Journal of Clinical Medicine in Practice, 2020, 24(9): 66-68. DOI: 10.7619/jcmp.202009019
    [10]XU Daxing, LI Sen, LI Yanjie, CHEN Linlin, WU Hao. Changes and significance of lactate dehydrogenase, adenosine deaminase and tumor necrosis factor-α in cerebrospinal fluid of patients with viral meningitis[J]. Journal of Clinical Medicine in Practice, 2016, (24): 26-28. DOI: 10.7619/jcmp.201624007

  • Cited by

    Periodical cited type(11)

    1. 朱丹，刘采连. 肝郁气滞型持续性姿势-感知性头晕患者运用柴胡疏肝散的疗效研究. 中外医学研究. 2025(06): 107-110 .
    2. 郭甜甜，彭壮，付晓芳. 逍遥解郁方联合前庭康复训练治疗持续性姿势-知觉性头晕临床观察. 实用中医药杂志. 2024(01): 39-41 .
    3. 张俊红，王丽，郑银燕，张佰景，张俊梅. 低频经颅磁刺激联合前庭康复训练治疗持续性姿势-感知性头晕的疗效观察. 中华物理医学与康复杂志. 2024(04): 317-321 .
    4. 史林娟，赵蕾，孙文青，宋红娜. 前庭康复训练联合艾司西酞普兰片治疗持续性姿势知觉性头晕的疗效. 慢性病学杂志. 2024(10): 1504-1506+1510 .
    5. 徐金凤，魏辰鸿，汪毅明，杨建波，魏建刚，张倩，林晓静，张小宁. 前庭康复训练法治疗中重度持续性姿势-感知性头晕的临床疗效分析. 中国临床神经科学. 2023(01): 43-48 .
    6. 贾云朋，王彩娟，纪朋曼，曹俊英，孟曙龙，田丹丹，赵扬，杨静棉. 灯银脑通胶囊治疗持续性姿势感知性头晕的作用机制研究. 世界中西医结合杂志. 2023(06): 1242-1245+1256 .
    7. 时钦，江炜炜，唐睩，刘红星，李峰，张丽. 草酸艾司西酞普兰配合视觉脱敏训练治疗老年持续性姿势-知觉性头晕的效果. 医学研究与战创伤救治. 2023(11): 1182-1187 .
    8. 马小琦. 持续性姿势知觉性头晕患者睡眠障碍与认知功能障碍相关性研究. 中国实用医药. 2022(04): 73-75 .
    9. 李锋森，孟毅，乔明亮，成家宏. 前庭康复训练联合药物疗法治疗持续性姿势-知觉性头晕的Meta分析. 神经损伤与功能重建. 2022(05): 264-268+306 .
    10. 张芳，俞晔，罗春香，杨敏，黄志英. 前庭康复训练应用于持续性姿势-知觉性头晕患者的临床研究. 中国医学创新. 2022(20): 150-153 .
    11. 钟伟森，张运如. 中医定向透药联合耳穴压豆治疗老年眩晕病的临床观察. 中医外治杂志. 2022(02): 19-21 .

    Other cited types(5)

Catalog

    Get Citation
    PDF
    XML
    Article views (261) PDF downloads (7) Cited by(16)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    © 2020 《实用临床医药杂志》编辑部

    Address: 江苏省扬州市江阳中路136号，扬州大学江阳路北校区14号楼201室China Pos: 225009Tel: 0514-87978917、87978989、87978807

    苏公网安备 32100302010246号

    Supported by: Beijing Renhe Information Technology Co., Ltd.   Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return