Value of fibronectin, vascular endothelial growth factor and lactate dehydrogenas levels in cerebrospinal fluid in diagnosing bacterial meningitis and viral encephalitis in children

DU Kaixian; HU Huihui; LI Lin; DONG Yan; GUAN Jing; SONG Chunlan; BAI Yanmin; KONG Lina; JIA Tianming

doi:10.7619/jcmp.20210272

Journal of Clinical Medicine in Practice > 2021 > 25(11): 26-30. > DOI: 10.7619/jcmp.20210272

DU Kaixian, HU Huihui, LI Lin, DONG Yan, GUAN Jing, SONG Chunlan, BAI Yanmin, KONG Lina, JIA Tianming. Value of fibronectin, vascular endothelial growth factor and lactate dehydrogenas levels in cerebrospinal fluid in diagnosing bacterial meningitis and viral encephalitis in children[J]. Journal of Clinical Medicine in Practice, 2021, 25(11): 26-30. DOI: 10.7619/jcmp.20210272

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Value of fibronectin, vascular endothelial growth factor and lactate dehydrogenas levels in cerebrospinal fluid in diagnosing bacterial meningitis and viral encephalitis in children

1.
Department of Neurology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052
2.
Department of Child Developmental Behavior, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052

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Received Date: January 13, 2021
Available Online: June 14, 2021
Published Date: June 14, 2021

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Abstract

Abstract

Objective To investigate the values of fibronectin(FN), vascular endothelial growth factor(VEGF) and lactate dehydrogenas(LDH) levels in cerebrospinal fluid in differential diagnosis of children with bacterial meningitis(BM) and viral encephalitis (VE).
Methods Thirty-one children with BM (BM group), thirty-four children with VE (VE group) and twenty-six children without central nervous system(CNS) infection (control group) were collected as research subjects. The levels of FN, VEGF and LDH in the cerebrospinal fluid of the three groups before and after treatment were detected by enzyme linked immunosorbent assay (ELISA); Receiver Operating Characteristic (ROC) curve was used to analyze the differential diagnostic values of FN, VEGF and LDH for BM and VE, the correlation of the three indicators were analyzed by Pearson coefficient.
Results Before treatment, the levels of FN, VEGF and LDH in the BM group were significantly higher than those in the control group and the VE group, and the level of FN in the VE group was significantly lower than that in the control group (P < 0.05). After treatment, the levels of FN, VEGF and LDH in the BM group were significantly decreased, and the levels of FN and LDH in the VE group were significantly decreased (P < 0.05). The area under the ROC curve (AUC) of FN, VEGF and LDH in differential diagnosis of the BM group and the VE group, the BM group and the control group were all more than 0.9, the AUC of FN in differential diagnosis of the VE group and the control group was more than 0.8, but the AUC of VEGF and LDH in differential diagnosis of the VE group and the control group were less than 0.6. There was positive correlations between FN, VEGF and LDH in the BM group, and a positive correlation between VEGF and LDH in the VE group, while there was no correlation between FN, VEGF and LDH in the control group.
Conclusion Detection the levels of FN, VEGF and LDH in cerebrospinal fluid has certain clinical value in the differential diagnosis of BM, VE and non-CNS infections.
- bacterial meningitis,
- viral encephalitis,
- vascular endothelial growth factor,
- lactate dehydrogenase,
- fibronectin,
- cerebrospinal fluid

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References

[1]	SINGHI P. Central nervous system infections in children: an ongoing challenge[J]. Indian J Pediatr, 2019, 86(1): 49-51. doi: 10.1007/s12098-018-2745-6
[2]	杜开先, 董燕, 张燕, 等. 肺炎链球菌脑膜炎大鼠脑组织水通道蛋白4表达的变化特点[J]. 中华实用儿科临床杂志, 2015, 30(7): 535-537. doi: 10.3760/cma.j.issn.2095-428X.2015.07.015
[3]	张林妹, 周水珍. 儿童中枢神经系统感染治疗疗程与腰椎穿刺检查系列建议之二: 细菌性脑膜炎治疗疗程与腰椎穿刺检查建议[J]. 中国实用儿科杂志, 2020, 35(1): 4-6. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSEK202001002.htm
[4]	杨慧湘. 病毒性脑炎患儿脑脊液Caveolin-1、IL-1β、VEGF的变化与意义[J]. 中国伤残医学, 2015, 23(7): 153-153, 166.
[5]	MORICHI S, MORISHITA N, TAKESHITA M, et al. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) levels in the cerebrospinal fluid of children with influenza-associated encephalopathy[J]. J Infect Chemother, 2017, 23(2): 80-84. doi: 10.1016/j.jiac.2016.10.007
[6]	王海如, 秦梓健, 王斌, 等. 新生儿化脓性脑膜炎并发脑白质损伤危险因素分析[J]. 临床儿科杂志, 2020, 38(2): 104-109. doi: 10.3969/j.issn.1000-3606.2020.02.007
[7]	江载芳, 申昆玲, 沈颖. 诸福棠实用儿科学[M]. 第8版. 北京: 人民卫生出版社, 2015: 135-140.
[8]	吕传真, 周良辅, 洪震. 实用神经病学[M]. 第4版. 上海: 上海科学技术出版社, 2014: 262-265.
[9]	SHYU L Y, HU M G, CHOU C H, et al. Fibronectin changes in eosinophilic meningitis with blood-CSF barrier disruption[J]. Exp Parasitol, 2015, 151/152: 73-79. doi: 10.1016/j.exppara.2015.02.002
[10]	杜开先, 张华玲, 李曼曼, 等. 病毒性脑炎及细菌性脑膜炎患儿血清及脑脊液血管内皮细胞钙黏蛋白、降钙素原的变化及意义[J]. 中华实用儿科临床杂志, 2019, 34(18): 1407-1410. doi: 10.3760/cma.j.issn.2095-428X.2019.18.012
[11]	KRAFT S, KLEMIS V, SENS C, et al. Identification and characterization of a unique role for EDB fibronectin in phagocytosis[J]. J Mol Med (Berl), 2016, 94(5): 567-581. doi: 10.1007/s00109-015-1373-0
[12]	LIU F, LI J Q, YAN K, et al. Binding of fibronectin to SsPepO facilitates the development of Streptococcus suis meningitis[J]. J Infect Dis, 2018, 217(6): 973-982. doi: 10.1093/infdis/jix523
[13]	PUPEK M, JASONEK J, KATNIK-PRASTOWSKA I. EDA-containing fibronectin levels in the cerebrospinal fluid of children with meningitis[J]. Ann Clin Lab Sci, 2013, 43(3): 257-266. http://europepmc.org/abstract/med/23884219
[14]	VAN DER FLIER M, STOCKHAMMER G, VONK G J, et al. Vascular endothelial growth factor in bacterial meningitis: detection in cerebrospinal fluid and localization in postmortem brain[J]. J Infect Dis, 2001, 183(1): 149-153. doi: 10.1086/317643
[15]	MISRA U K, KALITA J, SINGH A P, et al. Vascular endothelial growth factor in tuberculous meningitis[J]. Int J Neurosci, 2013, 123(2): 128-132. http://www.ncbi.nlm.nih.gov/pubmed/11412875
[16]	VIKSE J, HENRY B M, ROY J, et al. The role of serum procalcitonin in the diagnosis of bacterial meningitis in adults: a systematic review and meta-analysis[J]. Int J Infect Dis, 2015, 38: 68-76. doi: 10.1016/j.ijid.2015.07.011
[17]	KIM M, SOHN M, SHIM M, et al. Prognostic value of vascular endothelial growth factor (VEGF), VEGF receptor 2, platelet-derived growth factor-β (PDGF-β), and PDGF-β receptor expression in papillary renal cell carcinoma[J]. Hum Pathol, 2017, 61: 78-89. doi: 10.1016/j.humpath.2016.12.002
[18]	程大也, 梁彬. 脑脊液血管内皮生长因子(VEGF)检测在鉴别诊断细菌性脑膜炎和病毒性脑膜炎中的意义[J]. 现代预防医学, 2013, 40(6): 1107-1108, 1111. https://www.cnki.com.cn/Article/CJFDTOTAL-XDYF201306044.htm
[19]	徐宏斌, 盛小娟, 马建党, 等. 不同类型脑膜炎感染患儿脑脊液C-反应蛋白与乳酸相关指标变化分析[J]. 中华医院感染学杂志, 2016, 26(11): 2596-2598. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY201611065.htm
[20]	何万涛, 吴雪梅, 赵云红, 等. 中枢神经系统感染患儿脑脊液及血中白细胞计数、降钙素原、C-反应蛋白变化的相关性研究[J]. 中华医院感染学杂志, 2016, 26(18): 4219-4221. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY201618046.htm
[21]	徐大兴, 李森, 李艳杰, 等. 病毒性脑膜炎患者脑脊液中乳酸脱氢酶、腺苷脱氨酶及肿瘤坏死因子-α的水平变化及临床意义[J]. 实用临床医药杂志, 2016, 20(24): 26-28. doi: 10.7619/jcmp.201624007
[22]	胡瑞华. 不同类型脑膜炎感染患儿脑脊液C反应蛋白与乳酸相关指标变化分析[J]. 现代医用影像学, 2018, 27(5): 1807-1808, 1822. https://www.cnki.com.cn/Article/CJFDTOTAL-XDYY201805174.htm
[23]	熊学琴, 胡家胜, 刘智胜, 等. 乳酸脱氢酶和中性粒细胞CD64与脑脊液β2微球蛋白检测对小儿中枢神经系统感染的诊断分析[J]. 中华医院感染学杂志, 2017, 27(22): 5247-5250. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY201722057.htm

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Value of fibronectin, vascular endothelial growth factor and lactate dehydrogenas levels in cerebrospinal fluid in diagnosing bacterial meningitis and viral encephalitis in children