Objective To observe the expression of long non-coding RNA (lncRNA) cancer susceptibility candidate 11 (CASC11) in tissues of ovarian cancer, and to explore the effects of CASC11 gene on cellular proliferation, migration and invasive ability of SKOV3 cells.
Methods Real time fluorescent quantitative polymerase chain reaction (PCR) was used to detect the expressions of CASC11 gene in the tissues of ovarian cancer and adjacent tissues. The SKOV3 cells were divided into si-CASC11 group, negative control group and blank group, given interference sequence transfected by CASC11, negative control sequence and only transfection reagent only, respectively. The real time fluorescent quantitative PCR was used to detect the expression of CASC11 gene in the cells, and methyl tetrazolium (MTT) assay was used to detect cellular proliferation viability, scarification test was used to detect migration ability of the cells, and the Transwell chamber was used to detect invasive ability of cells.
Results Compared with the adjacent tissues, the relative expression level of CASC11 in ovarian cancer tissues was significantly higher (P<0.001). The relative expression of CASC11 showed significant difference in terms of degree of differentiation, Federation International of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis (P<0.05). Compared with the negative control group and the blank group, the relative expression level of CASC11, the cell proliferation, migration, and invasion capabilities in the cells of si-CASC11 group were significantly reduced (P<0.05 or P<0.01).
Conclusion The expression of CASC11 gene in ovarian cancer tissues is increased, and it is associated with the clinical pathological indicators of malignant progression. Inhibition of CASC11 expression can significantly inhibit the proliferation, migration and invasion of SKOV3 cell.
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