Mechanism of ropivacaine inhibiting the proliferation, migration and invasion of gastric cancer cell NCI-N87 through Wnt signaling pathway

CAO Qin; WANG Dan

doi:10.7619/jcmp.20211771

Journal of Clinical Medicine in Practice > 2021 > 25(20): 56-60, 67. > DOI: 10.7619/jcmp.20211771

CAO Qin, WANG Dan. Mechanism of ropivacaine inhibiting the proliferation, migration and invasion of gastric cancer cell NCI-N87 through Wnt signaling pathway[J]. Journal of Clinical Medicine in Practice, 2021, 25(20): 56-60, 67. DOI: 10.7619/jcmp.20211771

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Mechanism of ropivacaine inhibiting the proliferation, migration and invasion of gastric cancer cell NCI-N87 through Wnt signaling pathway

CAO Qin¹,
WANG Dan^2, ,

1.
Department of Anesthesiology, Chongqing Ninth People's Hospital, Chongqing, 400700
2.
Department of Gastroenterology, Chongqing Ninth People's Hospital, Chongqing, 400700

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Received Date: April 26, 2021
Available Online: November 15, 2021
Published Date: October 27, 2021

Graphical Abstract

Abstract

Abstract

Objective To investigate the mechanism of ropivacaine inhibiting the proliferation, migration and invasion of gastric cancer cell NCI-N87 through the Wnt signaling pathway.
Methods Gastric cancer cells NCI-N87 were cultured by ropivacaine cell culture fluid and were divided into control group (0 μg/mL ropivacaine), ROP-L group (160 μg/mL ropivacaine), ROP-M group (320 μg/mL ropivacaine), ROP-H group (640 μg/mL ropivacaine) and ROP-H+Licl (640 μg/mL ropivacaine, Wnt signal activator Licl) group. MTT assay was used to detect cell proliferation activity. Transwell assay was used to detect the changes in ability of cell migration and invasion. The protein expression levels of matrix metalloproteinase 9 (MMP-9), matrix metalloproteinase 2 (MMP-2), E-cadherin, β-catenin, N-cadherin and Wnt1 were analyzed by western blot.
Results Compared with the control group, the number of migration and invasion of gastric cancer cells in the ROP-L, ROP-M, and ROP-H groups gradually decreased, the proliferation activity decreased, the expression levels of MMP-2 and MMP-9 proteins in the cells gradually decreased, and the protein expression level of E-cadherin in the cells gradually increased, and the N-cadherin, Wnt1, and β-catenin protein expression levels gradually decreased (P < 0.05). Compared with the ROP-H group, the number of migration and invasion of gastric cancer cells in the ROP-H+Licl group increased, the protein levels of MMP-9, MMP-2, N-cadherin, Wnt1 and β-catenin increased, and the protein level of E-cadherin decreased (P < 0.05).
Conclusion Ropivacaine inhibits the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of gastric cancer cell NCI-N87 by down-regulating the Wnt signaling pathway.
- gastric cancer,
- ropivacaine,
- migration,
- invasion,
- Wnt signaling pathway,
- epithelial-mesenchymal transition

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References (23)

References

[1]	SHI S, YANG Z Z, LIU S, et al. PIWIL1 promotes gastric cancer via a PiRNA-independent mechanism[J]. PNAS, 2020, 117(36): 22390-22401. doi: 10.1073/pnas.2008724117
[2]	HUANG X, JIANG J Y, HUANG L J, et al. Ropivacaine prevents the activation of the NLRP3 inflammasome caused by high glucose in HUVECs[J]. ACS Omega, 2020, 5(36): 23413-23419. doi: 10.1021/acsomega.0c03143
[3]	CHEN X, LIU W X, GUO X H, et al. Ropivacaine inhibits cervical cancer cell growth via suppression of the miR-96/MEG2/pSTAT3 axis[J]. Oncol Rep, 2020, 43(5): 1659-1668.
[4]	WANG W T, ZHU M Y, XU Z X, et al. Ropivacaine promotes apoptosis of hepatocellular carcinoma cells through damaging mitochondria and activating caspase-3 activity[J]. Biol Res, 2019, 52(1): 36. doi: 10.1186/s40659-019-0242-7
[5]	ZHANG Y, PENG X, ZHENG Q. Ropivacaine inhibits the migration of esophageal cancer cells via sodium-channel-independent but prenylation-dependent inhibition of Rac1/JNK/paxillin/FAK[J]. Biochem Biophys Res Commun, 2018, 501(4): 1074-1079. doi: 10.1016/j.bbrc.2018.05.110
[6]	洪勇, 周民伟, 徐化交. 罗哌卡因经原癌基因信号通路抑制胃癌细胞增殖和迁移的机制[J]. 东南国防医药, 2020, 22(5): 456-460. doi: 10.3969/j.issn.1672-271X.2020.05.002
[7]	BUGTER J M, FENDERICO N, MAURICE M M. Mutations and mechanisms of WNT pathway tumour suppressors in cancer[J]. Nat Rev Cancer, 2021, 21(1): 5-21. doi: 10.1038/s41568-020-00307-z
[8]	HAN T, GOSWAMI S, HU Y, et al. Lineage reversion drives WNT independence in intestinal cancer[J]. Cancer Discov, 2020, 10(10): 1590-1609. doi: 10.1158/2159-8290.CD-19-1536
[9]	TOGASAKI K, SUGIMOTO S, OHTA Y, et al. Wnt signaling shapes the histologic variation in diffuse gastric cancer[J]. Gastroenterology, 2021, 160(3): 823-830. doi: 10.1053/j.gastro.2020.10.047
[10]	NICÁCIO I P, STELLE A B F, BRUNO T S, et al. Comparison of intraperitoneal ropivacaine and ropivacaine-dexmedetomidine for postoperative analgesia in cats undergoing ovariohysterectomy[J]. Vet Anaesth Analg, 2020, 47(3): 396-404. doi: 10.1016/j.vaa.2020.01.007
[11]	YANG W J, CAI J, ZHANG H M, et al. Effects of lidocaine and ropivacaine on gastric cancer cells through down-regulation of ERK1/2 phosphorylation in vitro[J]. Anticancer Res, 2018, 38(12): 6729-6735. doi: 10.21873/anticanres.13042
[12]	DOFARA S G, CHANG S L, DIORIO C. Gene polymorphisms and circulating levels of MMP-2 and MMP-9: a review of their role in breast cancer risk[J]. Anticancer Res, 2020, 40(7): 3619-3631. doi: 10.21873/anticanres.14351
[13]	PELTONEN R, HAGSTROM J, TERVAHARTIALA T, et al. High expression of MMP-9 in primary tumors and high preoperative MPO in serum predict improved prognosis in colorectal cancer with operable liver metastases[J]. Oncology, 2021, 99(3): 144-160.
[14]	LI H Q, ZHAO J, JIA X M, et al. miR-21 promotes growth, invasion and migration of lung cancer cells by AKT/P-AKT/cleaved-caspase 3/MMP-2/MMP-9 signaling pathway[J]. Int J Clin Exp Pathol, 2020, 13(4): 692-700.
[15]	JANG H Y, HONG O Y, YOUN H J, et al. 15d-PGJ2 inhibits NF-κB and AP-1-mediated MMP-9 expression and invasion of breast cancer cell by means of a heme oxygenase-1-dependent mechanism[J]. BMB Rep, 2020, 53(4): 212-217. doi: 10.5483/BMBRep.2020.53.4.164
[16]	WAN H, PETER T D, SARANTOS K, et al. TGF β-induced metabolic reprogramming during epithelial-to-mesenchymal transition in cancer[J]. Cellular and molecular life sciences: CMLS, 2020, 77(11): 2103-2123. doi: 10.1007/s00018-019-03398-6
[17]	YI H Y, LI G P, LONG Y K, et al. Integrative multi-omics analysis of a colon cancer cell line with heterogeneous Wnt activity revealed RUNX2 as an epigenetic regulator of EMT[J]. Oncogene, 2020, 39(28): 5152-5164. doi: 10.1038/s41388-020-1351-z
[18]	ROS M, SALA M, SALTEL F. Linking matrix rigidity with EMT and cancer invasion[J]. Dev Cell, 2020, 54(3): 293-295. doi: 10.1016/j.devcel.2020.06.032
[19]	ZHANG C P, HAO Y L, SUN Y Y, et al. Quercetin suppresses the tumorigenesis of oral squamous cell carcinoma by regulating microRNA-22/WNT1/β-catenin axis[J]. J Pharmacol Sci, 2019, 140(2): 128-136. doi: 10.1016/j.jphs.2019.03.005
[20]	GUO T, YUAN X, LIU D F, et al. LncRNA HOXA11-AS promotes migration and invasion through modulating miR-148a/WNT1/β-catenin pathway in gastric cancer[J]. Neoplasma, 2020, 67(3): 492-500.
[21]	YUE Z Y, YUAN Z J, ZENG L, et al. LGR4 modulates breast cancer initiation, metastasis, and cancer stem cells[J]. FASEB J, 2018, 32(5): 2422-2437. doi: 10.1096/fj.201700897R
[22]	LV J, FENG Z P, CHEN F K, et al. M2-like tumor-associated macrophages-secreted Wnt1 and Wnt3a promotes dedifferentiation and metastasis via activating β-catenin pathway in thyroid cancer[J]. Mol Carcinog, 2021, 60(1): 25-37. doi: 10.1002/mc.23268
[23]	ZHANG F, LI Y, XU W R, et al. Long non-coding RNA ZFAS1 regulates the malignant progression of gastric cancer via the microRNA-200b-3p/Wnt1 axis[J]. Biosci Biotechnol Biochem, 2019, 83(7): 1289-1299. doi: 10.1080/09168451.2019.1606697

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Mechanism of ropivacaine inhibiting the proliferation, migration and invasion of gastric cancer cell NCI-N87 through Wnt signaling pathway