Study on mechanism of LncRNA TBX5-AS1 in regulating proliferation, migration and invasion of colorectal cancer HCT116 cells by targeting miR-92a-3p

  Objective  To explore the effect of long non-coding RNA T-box transcription factor 5 antisense RNA 1 (LncRNA TBX5-AS1) on the biological behavior of colorectal cancer cells and its possible mechanism.

  Methods  Real time fluorescence quantitative polymerase chain reaction (qRT-PCR) method was used to detect the expression of TBX5-AS1 and miR-92a-3p in colorectal cancer tissues and adjacent tissues. Human colorectal cancer cell HCT116 was cultured in vitro, and TBX5-AS1 overexpression vector (pcDNA-TBX5-AS1) and its control empty vector (pcDNA), miR-92a-3p specific oligonucleotide inhibitor (anti-miR-92a-3p) and its negative control (anti-miR-NC), miR-92a-3p oligonucleotide mimics (miR-92a-3p mmics) and negative control mimic NC sequence (miR-NC), pcDNA-TBX5-AS1 and miR-92a-3p mimics were transfected into HCT116 cells. MTT method, plate colony formation experiment, scratch experiment and Transwell chamber experiment were used to detect cell proliferation, colony formation, migration and invasion ability respectively. The dual luciferase reporter gene experiment was used to detect the targeting relationship between TBX5-AS1 and miR-92a-3p.

  Results  Compared with adjacent tissues, the expression of TBX5-AS1 in colorectal cancer tissues decreased significantly, while the expression of miR-92a-3p increased significantly (P < 0.05). After transfection with pcDNA-TBX5-AS1 or anti-miR-92a-3p, cell viability decreased significantly, the number of colony formation and invasive cells decreased significantly, and the migration distance shortened significantly (P < 0.05). TBX5-AS1 was able to target miR-92a-3p. After transfection with miR-92a-3p mimics, cell viability increased significantly, the number of colony formation and invasive cells increased significantly, and the migration distance increased significantly (P < 0.05). Co-transfection with pcDNA-TBX5-AS1 and miR-92a-3p mmics was able to reverse the effect of pcDNA-TBX5-AS1 on the biological behavior of HCT116 cells.

  Conclusion  Overexpression of TBX5-AS1 can reduce the proliferation, colony formation, migration and invasion abilities of colorectal cancer cells by inhibiting the expression of miR-92a-3p.