Influence of RNA interference targeting Livin combined with cisplatin treatment on proliferation and apoptosis of Hep-2 cells in laryngeal squamous cell carcinoma

  Objective  To investigate the influence of RNA interference targeting Livin combined with cisplatin on proliferation and apoptosis of Hep-2 cells in laryngeal squamous cell carcinoma.

  Methods  One Hep-2 cell line of laryngeal squamous cell carcinoma was cultured in vitro, and cells in the logarithmic phase were collected and randomly divided into control group(without any treatment), interference group (transfected by RNA interference Livin), cisplatin group (treated with 6 μmol/L of cisplatin), and combined group (transfected with RNA interference Livin and 6 μmol/L of cisplatin), with 6 replicate holes for each group. After 48 hours of treatment, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to detect the inhibition rate of cell proliferation. Flow cytometry double staining was used to detect the apoptosis rate and cell cycle distribution. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of Bcl-2 mRNA and Bax mRNA.

  Results  The cell proliferation inhibition rate and apoptosis rate were higher in the combined group than those in the interference group and the cisplatin group (P < 0.05). The percentage of cells in G₀/G₁ phase was higher, and the percentages of cells in S phase and G₂/M phase were lower in the combined group than those in the interference group and the cisplatin group (P < 0.05). The Bcl-2 mRNA level was lower and the Bax mRNA level was higher in the combined group than those in the interference group and the cisplatin group (P < 0.05).

  Conclusion  The interference targeting Livin combined with cisplatin for Hep-2 cells in laryngeal squamous cell carcinoma can inhibit cell proliferation and promote cell apoptosis. The mechanism may be related to the regulation of apoptosis-related genes and the alteration of cell division cycle.