Effect of essential fatty acids by MCP-1/TGF-β<sub>1</sub>/COL-Ⅰ pathways for human kidney 2 cells with sugar damage

JIANG Mingxia; WANG Yu; LYU Guilan; ZHOU Yinan; DONG Xingyuan; XU Qi; ZHENG Jinfeng

doi:10.7619/jcmp.20220397

Journal of Clinical Medicine in Practice > 2022 > 26(13): 1-6, 40. > DOI: 10.7619/jcmp.20220397

JIANG Mingxia, WANG Yu, LYU Guilan, ZHOU Yinan, DONG Xingyuan, XU Qi, ZHENG Jinfeng. Effect of essential fatty acids by MCP-1/TGF-β₁/COL-Ⅰ pathways for human kidney 2 cells with sugar damage[J]. Journal of Clinical Medicine in Practice, 2022, 26(13): 1-6, 40. DOI: 10.7619/jcmp.20220397

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Effect of essential fatty acids by MCP-1/TGF-β₁/COL-Ⅰ pathways for human kidney 2 cells with sugar damage

1.
Department of Nutrition, General Hospital of East Theatre Command of Chinese People′s Liberation Army, Nanjing, Jiangsu, 210002
2.
National Clinical Research Center for Kidney Disease, General Hospital of East Theatre Command of Chinese People′s Liberation Army, Nanjing, Jiangsu, 210002

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Received Date: February 09, 2022
Available Online: July 01, 2022

Graphical Abstract

Abstract

Abstract

Objective
To study the effect and mechanism of monocyte chemotactic protein-1 (MCP-1)/transforming growth factor beta1(TGF-β₁)/collagen-Ⅰ (COL-Ⅰ) pathways for human kidney 2 (HK-2) cells induced by high glucose.
Methods
Methyl Thiazolyl Tetrazolium (MTT) method was used to establish HK-2 cell model damaged by sugar. The protein expression levels of MCP-1, TGF-β₁ and COL-Ⅰ in supernatant of HK-2 cells were determined by enzyme-linked immunosorbent assay (ELISA), and the expressions of MCP-1 , TGF-β₁ and COL-ⅠmRNA in HK-2 cells were determined by reverse transcription-polymerase chain reaction (RT-PCR) after treatment with different concentrations of alpha linolenic acid (ALA)/linoleic acid (LA).
Results
After 48 h intervention in HK-2 cells induced by glucose were treated with 50 μmol/L ALA and LA and 50 μmol/L mixture of ALA/LA with the ratio of 1 to 4, the expression levels of MCP-1mRNA and MCP-1 protein were significantly decreased (P < 0.05). After HK-2 cells induced by glucose and treated with 100 μmol/L ALA and LA for 48 h, TGF-β₁mRNA and TGF-β₁ protein expression were significantly decreased(P < 0.05). After HK-2 cells induced by glucose and treated with 100 μmol/L ALA, 50 μmol/L LA and 50 μmol/L mixture of ALA/LA with the ratio of 1 ∶ 4 for 48 h, the expression of COL-Ⅰ mRNA and COL-Ⅰ protein were significantly decreased (P < 0.05).
Conclusion
HK-2 cells are damaged by high glucose, manifesting as increased expression of cytokines MCP-1 and TGF-β₁, and increased expression of fibrosis product COL-Ⅰ. ALA/LA can down-regulate the expression of MCP-1 and TGF-β₁, reduce the level of COL-Ⅰ, and protect HK-2 cells damaged by glucose.

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References (15)

References

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Effect of essential fatty acids by MCP-1/TGF-β1/COL-Ⅰ pathways for human kidney 2 cells with sugar damage

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Effect of essential fatty acids by MCP-1/TGF-β₁/COL-Ⅰ pathways for human kidney 2 cells with sugar damage