Role and mechanism of developmental endothelial locus-1 in collagen-induced arthritis

WEI Yu; XU Xuefeng; TANG Xiaojun; LIANG Jun; FENG Xuebing; ZHAO Cheng

doi:10.7619/jcmp.20220543

Journal of Clinical Medicine in Practice > 2022 > 26(17): 53-56. > DOI: 10.7619/jcmp.20220543

WEI Yu, XU Xuefeng, TANG Xiaojun, LIANG Jun, FENG Xuebing, ZHAO Cheng. Role and mechanism of developmental endothelial locus-1 in collagen-induced arthritis[J]. Journal of Clinical Medicine in Practice, 2022, 26(17): 53-56. DOI: 10.7619/jcmp.20220543

Citation:

PDF (1521 KB)

Role and mechanism of developmental endothelial locus-1 in collagen-induced arthritis

Department of Rheumatology and Immunology, Drum Tower Hospital Affiliated to Medical College of Nanjing University, Nanjing, Jiangsu, 210008

More Information

Received Date: February 21, 2022
Available Online: September 20, 2022

Graphical Abstract

Abstract

Abstract

Objective
To investigate the role and mechanism of developmental endothelial locus-1 (Del-1) in collagen-induced arthritis (CIA).
Methods
CIA mouse model was constructed, and the mice were divided into control group, CIA group, and Del-1 group (Del-1 for intervention of CIA). In the Del-1 group, each mouse was injected with Del-1 for 2 μg each time through caudal vein for 2 times in total. Arthritis index (AI) was used to assess the progression of arthritis; hematoxylin-eosin (HE) staining was used to observe the arthritic lesions in mice; enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of serum interleukin-6 (IL-6) and matrix metalloproteinases-9 (MMP-9) in mice.
Results
On the 34th, 36th, 38th and 40th days after the primary immunization, the AI scores in the Del-1 group were (3.80±1.17), (3.80±1.17), (4.20±1.17) and (4.20±1.17) respectively, which were significantly lower than (6.80±1.94), (8.80±3.19), (9.60±3.61) and (9.40±3.61) in the CIA group (P < 0.05). On the 42nd day after the primary immunization, when compared to the CIA group, the HE staining showed that the local inflammatory infiltration and synovial hyperplasia of the joints in the Del-1 group were obviously alleviated, and the cartilage destruction was obviously reduced. On the 42nd day after the initial immunization, ELISA result showed that the expression levels of serum IL-6 and MMP-9 in the CIA group were significantly higher than that in the normal control group (P < 0.05), while those in the Del-1 group were significantly lower than the CIA group (P < 0.05).
Conclusion
Del-1 can improve the joint symptoms of CIA mouse model, reduce inflammation and bone destruction, inhibit the expression of key cytokines such as IL-6 and MMP-9, and these actions may play certain roles in the treatment of RA.
- developmental endothelial locus-1,
- collagen-induced arthritis,
- rheumatoid arthritis,
- interleukin-6,
- matrix metalloproteinase

FullText(HTML)

References (15)

References

[1]	JIANG Q, WANG X, HUANG E Y, et al. Inflammasome and its therapeutic targeting in rheumatoid arthritis[J]. Front Immunol, 2022, 12: 816839. doi: 10.3389/fimmu.2021.816839
[2]	HUANG J, FU X K, CHEN X X, et al. Promising therapeutic targets for treatment of rheumatoid arthritis[J]. Front Immunol, 2021, 12: 686155. doi: 10.3389/fimmu.2021.686155
[3]	SHIN J, MAEKAWA T, ABE T, et al. DEL-1 restrains osteoclastogenesis and inhibits inflammatory bone loss in nonhuman Primates[J]. Sci Transl Med, 2015, 7(307): 307ra155. https://www.uab.edu/medicine/rheumatology/images/JJ_-_Shin_et_al_2015.pdf
[4]	LIU C, ZHANG H Y, TANG X J, et al. Mesenchymal stem cells promote the osteogenesis in collagen-induced arthritic mice through the inhibition of TNF- Α[J]. Stem Cells Int, 2018, 2018: 4069032. https://www.hindawi.com/journals/sci/2018/4069032/
[5]	赵成, 张璐, 王红, 等. 脐带间充质干细胞移植治疗大鼠胶原诱导关节炎模型的疗效观察[J]. 实用临床医药杂志, 2015, 19(9): 1-4, 8. doi: 10.7619/jcmp.201509001
[6]	HAJISHENGALLIS G, CHAVAKIS T. DEL-1: a potential therapeutic target in inflammatory and autoimmune disease[J]. Expert Rev Clin Immunol, 2021, 17(6): 549-552. doi: 10.1080/1744666X.2021.1915771
[7]	LI M, ZHONG D, LI G Z. Regulatory role of local tissue signal Del-1 in cancer and inflammation: a review[J]. Cell Mol Biol Lett, 2021, 26(1): 31. doi: 10.1186/s11658-021-00274-9
[8]	HAJISHENGALLIS G, CHAVAKIS T. DEL-1-regulated immune plasticity and inflammatory disorders[J]. Trends Mol Med, 2019, 25(5): 444-459. doi: 10.1016/j.molmed.2019.02.010
[9]	NARAZAKI M, TANAKA T, KISHIMOTO T. The role and therapeutic targeting of IL-6 in rheumatoid arthritis[J]. Expert Rev Clin Immunol, 2017, 13(6): 535-551. doi: 10.1080/1744666X.2017.1295850
[10]	NOACK M, MIOSSEC P. Selected cytokine pathways in rheumatoid arthritis[J]. Semin Immunopathol, 2017, 39(4): 365-383. doi: 10.1007/s00281-017-0619-z
[11]	HASHIZUME M, MIHARA M. The roles of interleukin-6 in the pathogenesis of rheumatoid arthritis[J]. Arthritis, 2011, 2011: 765624. https://www.hindawi.com/journals/arthritis/2011/765624/
[12]	FUJIMOTO M, SERADA S, MIHARA M, et al. Interleukin-6 blockade suppresses autoimmune arthritis in mice by the inhibition of inflammatory Th17 responses[J]. Arthritis Rheum, 2008, 58(12): 3710-3719. doi: 10.1002/art.24126
[13]	PANDOLFI F, FRANZA L, CARUSI V, et al. Interleukin-6 in rheumatoid arthritis[J]. Int J Mol Sci, 2020, 21(15): 5238. doi: 10.3390/ijms21155238
[14]	GRILLET B, YU K R, UGARTE-BERZAL E, et al. Proteoform analysis of matrix metalloproteinase-9/gelatinase B and discovery of its citrullination in rheumatoid arthritis synovial fluids[J]. Front Immunol, 2021, 12: 763832. doi: 10.3389/fimmu.2021.763832
[15]	KRABBEN A, HUIZINGA T W J, MIL A H. Biomarkers for radiographic progression in rheumatoid arthritis[J]. Curr Pharm Des, 2015, 21(2): 147-169.

[1]	LIU Bin, ZHANG Min, LI Xiangang, YANG Zhimin, ZHOU Fang, HUANG Chenbin, CHEN Yangbao, YANG Demei. Correlation between single nucleotide polymorphism rs1333049 and susceptibility to coronary heart disease of Yi ethnic people in frontier plateau area[J]. Journal of Clinical Medicine in Practice, 2022, 26(17): 24-28, 36. DOI: 10.7619/jcmp.20220678
[2]	ZHANG Xinyun, WANG Zehua, YAO Siyu. Associations of miR-146a polymorphism with diabetic peripheral neuropathy and clinical effect[J]. Journal of Clinical Medicine in Practice, 2022, 26(1): 71-75, 94. DOI: 10.7619/jcmp.20213887
[3]	MAO Weidong, ZHOU Zhihui, ZHANG Yanmei. Correlation between single nucleotide polymorphisms of TLR7 gene and susceptibility to esophageal cancer[J]. Journal of Clinical Medicine in Practice, 2017, (5): 102-105. DOI: 10.7619/jcmp.201705028
[4]	YUN Zhelin, WANG Dongmei, QU Yanjie. Relationship between bronchial asthma and glucocorticoid receptor gene bcl-I single nucleotide polymorphism[J]. Journal of Clinical Medicine in Practice, 2016, (11): 21-24. DOI: 10.7619/jcmp.201611007
[5]	QU Yanli, YU Hong, ZHAO Yuxia, SUN Deyu, CHEN Guangjun, LIU Dan. Role of IL-1 gene single nucleotide polymorphisms in the occurrence and development of nasopharyngeal carcinoma[J]. Journal of Clinical Medicine in Practice, 2016, (5): 92-95. DOI: 10.7619/jcmp.201605028
[6]	PEI Jianzhong, ZHANG Hui, WANG Mingjie, ZHANG Zhengyu, LIU Ruiping, ZHOU Xindie. Correlation between Caspase-7 (rs7907519, rs3127075) and IL1A (rs1800587) polymorphisms and risks of rheumatoid arthritis[J]. Journal of Clinical Medicine in Practice, 2015, (19): 1-4,12. DOI: 10.7619/jcmp.201519001
[7]	CAO Quanfu, YE Jin, FU Chengwei, FENG Qingxing, ZHANG Jun, ZHANG Keqin. Analysis in correlation between polymorphism of IL-23R gene rs10889677 and susceptibility of bladder cancer in Chongqing area[J]. Journal of Clinical Medicine in Practice, 2015, (9): 73-75,83. DOI: 10.7619/jcmp.201509021
[8]	QU Tao, ZHANG Honggang, CUI Chengxu, YANG Lin. Correlation between CYP2A6 polymorphisms and clinical outcome of S-1-based chemotherapy in patients with advanced cancer: A meta-analysis[J]. Journal of Clinical Medicine in Practice, 2015, (3): 15-19. DOI: 10.7619/jcmp.201503004
[9]	QIU Gang, ZHU Chang, LIU Xiaochun. Correlation between single nucleotide polymorphism and susceptibility of gastric cancer[J]. Journal of Clinical Medicine in Practice, 2014, (19): 59-61,65. DOI: 10.7619/jcmp.201419018
[10]	LIU Xin-zi, QIAN Xiao-ping, LIU Bao-rui, HU Wen-jing, WANG Li-feng, WEI Jia, YU Li-Xia. Single nucleotide polymorphisms in XRCCl,XPD and platinum prognosis in non-small-cell lung cancer patients[J]. Journal of Clinical Medicine in Practice, 2008, (9): 7-11,18. DOI: 10.3969/j.issn.1672-2353.2008.09.002

Cited By

Cited by

Periodical cited type(2)

1.	王依玟，史亚军，孟倩，白敏，崔娜，文爱东，王婧雯，丁一. 基于数据挖掘研究中医药治疗缺血性脑血管疾病的用药规律. 空军军医大学学报. 2024(09): 1042-1048+1054 .
2.	徐文立，段小花，陈普，杨丽萍. 针药结合治疗缺血性脑卒中的研究进展. 云南中医中药杂志. 2023(10): 63-69 .

Other cited types(1)

Get Citation

PDF

XML

Article views (201) PDF downloads (14) Cited by(3)

Turn off MathJax

Article Contents

Abstract

References

Role and mechanism of developmental endothelial locus-1 in collagen-induced arthritis