Relationships of BTB/POZ domain-containing protein 7 and matrix metalloproteinase-9 expression with autoantibodies in labial gland tissues of patients with primary Sjögren's syndrome

WANG Fei; SHI Meihong

doi:10.7619/jcmp.20220899

Journal of Clinical Medicine in Practice > 2022 > 26(21): 98-102. > DOI: 10.7619/jcmp.20220899

WANG Fei, SHI Meihong. Relationships of BTB/POZ domain-containing protein 7 and matrix metalloproteinase-9 expression with autoantibodies in labial gland tissues of patients with primary Sjögren's syndrome[J]. Journal of Clinical Medicine in Practice, 2022, 26(21): 98-102. DOI: 10.7619/jcmp.20220899

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Relationships of BTB/POZ domain-containing protein 7 and matrix metalloproteinase-9 expression with autoantibodies in labial gland tissues of patients with primary Sjögren's syndrome

WANG Fei,
SHI Meihong^,

Department of Laboratory, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, 225100

More Information

Received Date: March 21, 2022
Available Online: November 17, 2022

Graphical Abstract

Abstract

Abstract

Objective
To investigate the relationships of BTB/POZ domain-containing protein 7 (BTBD7) and matrix metalloproteinase-9 (MMP-9) expression with autoantibodies in labial gland tissues of patients with primary SjÖgren′s syndrome (pSS).
Methods
From September 2016 to October 2020, the labial gland tissues of 46 patients with pSS were collected as pSS group, and the labial gland tissues of 46 patients with xerostomia in the same period were collected as control group. The mRNA and protein expressions of BTBD7 and MMP-9 in labial gland tissues were detected; the relationships of the serum autoantibodies [anti-SjÖgren′s syndrome antigen A (SSA) antibody, anti-SjÖgren′s syndrome antigen B (SSB) antibody, and antinuclear antibody (ANA)] with BTBD7 and MMP-9 were analyzed in patients with pSS; the correlation between protein expression level of BTBD7 and protein expression level of MMP-9 was analyzed.
Results
Compared with the control group, the mRNA and protein expression levels of BTBD7 and MMP-9 in the labial gland tissues of patients in the pSS group were significantly higher (P < 0.05). In the detection results of anti-SSA antibody, anti-SSB antibody and ANA antibody in the patients with pSS, the mRNA and protein expression levels of BTBD7 and MMP-9 in the labial gland tissues of positive patients were significantly higher than those of negative patients (P < 0.05). The correlation analysis showed that there was a significant positive correlation between protein expression level of BTBD7 and protein expression level of MMP-9 in the labial gland tissues of patients with pSS (r=0.493, P < 0.05).
Conclusion
BTBD7 and MMP-9 are highly expressed in the labial gland tissues of patients with pSS, and are closely related to the expressions of autoantibodies such as anti-SSA antibody, anti-SSB antibody and ANA.
- primary SjÖgren's syndrome,
- labial gland tissues,
- BTB/POZ domain-containing protein 7,
- matrix metalloproteinase-9,
- autoantibodies

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[1]	高雅静, 王慧, 王永福. B细胞在原发性干燥综合征发病机制及治疗中的研究进展[J]. 实用临床医药杂志, 2021, 25(14): 108-112. doi: 10.7619/jcmp.20211343
[2]	赵小莹, 白宁, 陈群, 等. 干燥综合征患者血清抗α-胞衬蛋白、抗SSA/SSB抗体、IFN-γ及TNF-α与唇腺病理损害相关性研究[J]. 陕西医学杂志, 2020, 49(2): 241-246. doi: 10.3969/j.issn.1000-7377.2020.02.031
[3]	罗静, 陈嘉琪, 黄子玮, 等. 原发性干燥综合征继发血细胞减少和间质性肺病相关因素分析[J]. 中华风湿病学杂志, 2021, 25(6): 393-397. doi: 10.3760/cma.j.cn141217-20201225-00495
[4]	陈伟钱, 戴小娜, 余叶, 等. 原发性干燥综合征合并自身免疫性肝病的临床特点及预后分析[J]. 北京大学学报: 医学版, 2020, 52(5): 886-891. https://www.cnki.com.cn/Article/CJFDTOTAL-BYDB202005016.htm
[5]	CHEN B, LIU C, BAI G H, et al. BTBD7 accelerates the epithelial-mesenchymal transition, proliferation and invasion of prostate cancer cells[J]. J BUON, 2021, 26(5): 2111-2116.
[6]	王梦洁, 徐子琦, 刘英. 原发性干燥综合征的中西医治疗新进展[J]. 现代中西医结合杂志, 2021, 30(4): 443-448. doi: 10.3969/j.issn.1008-8849.2021.04.023
[7]	RIANI M, MULLER C, BOUR C, et al. Blister fluid induces MMP-9-associated M2-type macrophages in bullous pemphigoid[J]. Front Immunol, 2019, 10: 1858. doi: 10.3389/fimmu.2019.01858
[8]	MAO Y M, WANG S, ZHAO C N, et al. Circulating matrix metalloproteinase-9 levels in patients with systemic lupus erythematosus: a meta-analysis[J]. Curr Pharm Des, 2018, 24(16): 1780-1787. doi: 10.2174/1381612824666180607123431
[9]	GARRETO L, CHARNEAU S, MANDACARU S C, et al. Mapping salivary proteases in sjögren's syndrome patients reveals overexpression of dipeptidyl peptidase-4/CD26[J]. Front Immunol, 2021, 12: 686480. doi: 10.3389/fimmu.2021.686480
[10]	SHIBOSKI C H, SHIBOSKI S C, SEROR R, et al. 2016 American college of rheumatology/European league against rheumatism classification criteria for primary sjögren's syndrome: a consensus and data-driven methodology involving three international patient cohorts[J]. Arthritis Rheumatol, 2017, 69(1): 35-45. doi: 10.1002/art.39859
[11]	林进, 陈伟钱. 原发性干燥综合征的诊断[J]. 内科理论与实践, 2016, 11(6): 352-355. https://www.cnki.com.cn/Article/CJFDTOTAL-NKLL201606006.htm
[12]	高波, 周磊, 高泉. 伴有血小板减少的原发性干燥综合征患者的临床特点分析[J]. 实用临床医药杂志, 2016, 20(3): 176-177. doi: 10.7619/jcmp.201603066
[13]	曹京旌, 宁晓然, 闫永龙. 干燥综合征患者血液系统受损与自身抗体相关性研究[J]. 河北医药, 2018, 40(6): 882-885, 889. https://www.cnki.com.cn/Article/CJFDTOTAL-HBYZ201806019.htm
[14]	张博纶, 高明利. 高明利益气养阴通络法治疗类风湿关节炎继发干燥综合征经验[J]. 辽宁中医杂志, 2020, 47(12): 51-54. https://www.cnki.com.cn/Article/CJFDTOTAL-LNZY202012014.htm
[15]	侯佳奇, 吴香香, 潘云翠, 等. IFN-γ, caspase-3, LC3-Ⅱ在原发性干燥综合征唇腺中的表达及其相关性[J]. 临床与病理杂志, 2019, 39(4): 769-775. https://www.cnki.com.cn/Article/CJFDTOTAL-WYSB201904013.htm
[16]	张硕, 高尔涵, 达林泰, 等. BST-2在pSS患者唇腺、外周血淋巴细胞中的表达及增殖作用[J]. 国际检验医学杂志, 2020, 41(5): 544-547. doi: 10.3969/j.issn.1673-4130.2020.05.008
[17]	张科, 何雪春, 陈超, 等. 原发性干燥综合征相关间质性肺疾病的发生率及危险因素分析[J]. 实用临床医药杂志, 2021, 25(18): 10-14. doi: 10.7619/jcmp.20212574
[18]	CHEN J, LAI Y H, OOI S, et al. BTB domain-containing 7 predicts low recurrence and suppresses tumor progression by deactivating Notch1 signaling in breast cancer[J]. Breast Cancer Res Treat, 2020, 184(2): 287-300.
[19]	杜望磊, 杨西超, 谢荣华, 等. 原发性干燥综合征患者唇腺组织BAFF、MMP-9和E-cad表达水平及其临床意义[J]. 解放军医药杂志, 2017, 29(3): 73-75, 79. https://www.cnki.com.cn/Article/CJFDTOTAL-HBGF201703020.htm
[20]	赫志强, 王雷, 杨锦建. 长链非编码RNA氧化应激反应丝氨酸丰富1反义RNA 1对肾癌细胞增殖、迁移及侵袭的影响[J]. 中华实验外科杂志, 2021, 38(3): 488-492. https://www.cnki.com.cn/Article/CJFDTOTAL-XZYX202110002.htm
[21]	AOTA K, ONO S, YAMANOI T, et al. MMP-9 inhibition suppresses interferon-γ-induced CXCL10 production in human salivary gland ductal cells[J]. Inflammation, 2019, 42(6): 2148-2158.
[22]	SHAH N R, NOLL B D, PADILLA R J, et al. Expression of ETS1 and LEF1 in salivary glands of Sjögren syndrome patients[J]. Oral Dis, 2019, 25(1): 164-173.

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Relationships of BTB/POZ domain-containing protein 7 and matrix metalloproteinase-9 expression with autoantibodies in labial gland tissues of patients with primary Sjögren's syndrome

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