Influencing factors of false negative of T-cell spot test for tuberculosis infection

LIN Chujuan; XU Xiaopan; GUO Lan

doi:10.7619/jcmp.20221314

Journal of Clinical Medicine in Practice > 2022 > 26(19): 96-99, 104. > DOI: 10.7619/jcmp.20221314

LIN Chujuan, XU Xiaopan, GUO Lan. Influencing factors of false negative of T-cell spot test for tuberculosis infection[J]. Journal of Clinical Medicine in Practice, 2022, 26(19): 96-99, 104. DOI: 10.7619/jcmp.20221314

Citation:

PDF (661 KB)

Influencing factors of false negative of T-cell spot test for tuberculosis infection

1.
School of Public Health of Sun Yat-sen University, Guangzhou, Guangdong, 510000
2.
Department of Clinical Laboratory, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, 519000

More Information

Received Date: April 21, 2022
Available Online: October 23, 2022

Graphical Abstract

Abstract

Abstract

Objective
To investigate the influencing factors of false negative of T-cell spot test for tuberculosis infection (T-SPOT.TB).
Methods
A total of 473 patients diagnosed with active pulmonary tuberculosis (APTB) in the Fifth Affiliated Hospital Affiliated to Sun Yat-sen University from October 2019 to April 2021 were selected, and divided into negative group (78 cases) and positive group (395 cases) according to T-SPOT.TB results. The demographic characteristics, clinical characteristics and laboratory results of the two groups were compared, and the influencing factors of false negative T-SPOT.TB were screened; the multivariate Logistic regression analysis was used to screen the independent influencing factors of false negative T-SPOT.TB.
Results
The results of T-SPOT.TB were not affected by gender, negative smear, initial treatment or retreatment, imaging findings with or without cavities, diabetes mellitus and tuberculous pleurisy. Older age, increased urea, decreased total protein and albumin were the influencing factors of false negative T-SPOT.TB. The false negative rates of T-SPOT.TB in APTB patients ≤25 years old, 26 to 45 years old, 46 to 65 years old and >65 years old were 5.88%, 11.51%, 19.19% and 26.60%, respectively, showing an increasing trend (P < 0.001). Spearman rank correlation analysis showed that the definition values of each age group were negatively correlated with T-SPOT.TB results (r=-0.183, P < 0.001). Multivariate Logistic regression analysis showed that age >65 years was an independent influencing factor for false negative T-SPOT.TB. Compared with age ≤25 years old, the OR value of false negative T-SPOT.TB.in age>65 years old was 3.896 (95%CI, 1.228~12.361), P=0.021.
Conclusion
Age>65 years of APTB patients is an independent risk factor for false negative T-SPOT.TB.
- active pulmonary tuberculosis,
- T-cell spot test for tuberculosis infection,
- false negative,
- age

FullText(HTML)

References (20)

References

[1]	FUKUNAGA R, GLAZIOU P, HARRIS J B, et al. Epidemiology of tuberculosis and progress toward meeting global targets-worldwide, 2019[J]. MMWR Morb Mortal Wkly Rep, 2021, 70(12): 427-430. doi: 10.15585/mmwr.mm7012a4
[2]	中华人民共和国国家卫生和计划生育委员会. 肺结核诊断标准(WS 288—2017)[J]. 新发传染病电子杂志, 2018, 3(1): 59-61. https://www.cnki.com.cn/Article/CJFDTOTAL-ZFLZ202009005.htm
[3]	UDA S, ONO S, YUBA T, et al. Clinical features in active tuberculosis patients with effects on T-SPOT. TB assay results[J]. Infect Dis (Lond), 2020, 52(1): 58-60 doi: 10.1080/23744235.2019.1663363
[4]	ZHU M, ZHU Z J, YANG J Z, et al. Performance evaluation of IGRA-ELISA and T-SPOT. TB for diagnosing tuberculosis infection[J]. Clin Lab, 2019, 65(8): 7.
[5]	宋洋, 翁绳凤, 郭威, 等. 结核感染T细胞斑点试验的临床应用价值探讨[J]. 医学动物防制, 2020, 36(6): 607-610. https://www.cnki.com.cn/Article/CJFDTOTAL-YXDZ202006029.htm
[6]	夏青, 厉虹淼, 侯婧. 结核感染T细胞斑点试验对老年肺结核的诊断价值[J]. 实用临床医药杂志, 2021, 25(16): 18-21. doi: 10.7619/jcmp.20212162
[7]	许敏殷, 刘银, 刘丽蓉, 等. γ干扰素释放试验联合IL-6检测对活动性肺结核的诊断价值[J]. 中国现代医学杂志, 2020, 30(10): 18-21. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXDY202010005.htm
[8]	宋瑞红, 沈魏, 鲁立文. 结核感染T细胞斑点试验在肺结核和肺外结核诊断中的价值[J]. 中国医药导报, 2019, 16(1): 157-161. https://www.cnki.com.cn/Article/CJFDTOTAL-YYCY201901040.htm
[9]	SHANGGUAN Y W, FANG H, WANG S T, et al. Risk factors for negative T-SPOT. TB assay results in patients with confirmed active tuberculosis: a retrospective study[J]. J Infect Dev Ctries, 2020, 14(11): 1288-1295.
[10]	KANG W L, WU M Y, YANG K Y, et al. Factors associated with negative T-SPOT. TB results among smear-negative tuberculosis patients in China[J]. Sci Rep, 2018, 8(1): 4236.
[11]	夏青, 桂淑玉, 韩莙. 结核感染T细胞斑点试验在结核病诊断中的价值及影响因素[J]. 实用医学杂志, 2018, 34(20): 3469-3473. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYZ201820035.htm
[12]	卢青纯, 唐小燕, 熊恬园, 等. T-SPOT. TB试验在活动性结核诊断中的价值及其影响因素分析[J]. 中国循证医学杂志, 2014, 14(5): 522-526. https://www.cnki.com.cn/Article/CJFDTOTAL-ZZXZ201405006.htm
[13]	杨牧青. 肺结核合并糖尿病的临床分析及T-SPOT. TB对肺结核合并糖尿病患者的诊断价值[D]. 郑州: 郑州大学, 2018.
[14]	梁丽丽, 吕娅敏, 马芸, 等. 结核感染T细胞斑点试验假阴性影响因素回归分析[J]. 医学研究杂志, 2019, 48(1): 42-46. https://www.cnki.com.cn/Article/CJFDTOTAL-YXYZ201901014.htm
[15]	张群. T-SPOT. TB对结核病诊断敏感性及其影响因素的探讨[D]. 济南: 山东大学, 2016.
[16]	LEE Y M, PARK K H, KIM S M, et al. Risk factors for false-negative results of T-SPOT. TB and tuberculin skin test in extrapulmonary tuberculosis[J]. Infection, 2013, 41(6): 1089-1095.
[17]	陶学芳, 王华钧, 王建华, 等. 活动性肺结核患者外周血T-SPOT. TB测定与细菌学及影像学的关系[J]. 浙江医学, 2013, 35(23): 2057-2060, 2064. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJYE201323008.htm
[18]	罗卫红, 曹刚, 田斌. 年龄因素对肺结核患者T-SPOT. TB试验灵敏度的影响[J]. 国际检验医学杂志, 2017, 38(18): 2638-2639. https://www.cnki.com.cn/Article/CJFDTOTAL-GWSQ201718054.htm
[19]	陈卉, 孙彦波, 沈鑫, 等. 老年人群肺结核主动发现及患者特征分析[J]. 中国防痨杂志, 2021, 43(6): 550-556. https://www.cnki.com.cn/Article/CJFDTOTAL-ZFLZ202106007.htm
[20]	王黎霞, 成诗明, 陈明亭, 等. 2010年全国第五次结核病流行病学抽样调查报告[J]. 中国防痨杂志, 2012, 34(8): 485-508. https://www.cnki.com.cn/Article/CJFDTOTAL-ZFLZ201208002.htm

[1]	FANG Zhiqin, LIANG Huiqing, ZHANG Pengxiang, ZHAO Kun, MA Yiping, WANG Yaling, LI Fangjiang, LI Jun, LI Pingping. Effect of nicorandil combined with rosuvastatin calcium in treatment of patients with slow coronary flow[J]. Journal of Clinical Medicine in Practice, 2024, 28(15): 70-75. DOI: 10.7619/jcmp.20241838
[2]	SONG Jin. Clinical effect of butylphthalide injection combined with rosuvastatin calcium tablets in the treatment of acute progressive cerebral infarction[J]. Journal of Clinical Medicine in Practice, 2019, 23(17): 45-47, 51. DOI: 10.7619/jcmp.201917013
[3]	YE Lei, WEN Li. Effect of rosuvastatin on vascular endothelial function, cardiac function and hemodynamics in patients with coronary heart disease[J]. Journal of Clinical Medicine in Practice, 2018, (1): 18-20. DOI: 10.7619/jcmp.201801006
[4]	XIAO Ru'nan. Efficacy of nursing intervention in the treatment of amlodipine and atorvastatin calcium tablet for patients with hypertension combined with coronary heart disease[J]. Journal of Clinical Medicine in Practice, 2017, (4): 1-3,7. DOI: 10.7619/jcmp.201704001
[5]	JU Yanling, ZANG Xuelian, ZHAO Xu. Effect of amlodipine atorvastatin calcium tablet on IMT, PV, TNF, CRP, and blood lipid level of coronary heart disease and high blood pressure patients complicated with carotid atherosclerosis[J]. Journal of Clinical Medicine in Practice, 2017, (1): 14-18. DOI: 10.7619/jcmp.201701004
[6]	LIU Juan, ZHANG Bo, ZHANG Xiaoli, TANG Lingzhi. Influence of amlodipine combined with rosuvastatin calcium on CRP and D-dimer in elderly patients with acute cerebral infarction complicated with hypertension[J]. Journal of Clinical Medicine in Practice, 2016, (23): 9-12. DOI: 10.7619/jcmp.201623003
[7]	LIANG Tao, JIANG Junsong. Effect comparison between rosuvastatin and atorvastatin on the myocardial damage and levels of inflammatory factors in patients with unstable angina pectoris in perioperative period of percutaneous coronary intervention[J]. Journal of Clinical Medicine in Practice, 2016, (3): 18-20,24. DOI: 10.7619/jcmp.201603006
[8]	TANG Bo. Clinical effect of rosuvastatin versus atorvastatin in treatment of coronary heart disease[J]. Journal of Clinical Medicine in Practice, 2015, (Z1): 168-169.
[9]	WANG Xin, ZHAO Donghui. Influence of different doses of rosuvastatin on serum hs-CRP and PAPP-A level after stents implantation in patients with coronary heart disease[J]. Journal of Clinical Medicine in Practice, 2014, (19): 1-3. DOI: 10.7619/jcmp.201419001
[10]	XU Weidong, MIN Zhaohan. Effects of rosuvastatin on the levels of blood glucose, blood lipid and hypersensitive C-reactive protein in patients with hypertension[J]. Journal of Clinical Medicine in Practice, 2013, (7): 27-28,32. DOI: 10.7619/jcmp.201307008

Cited By

Cited by

Periodical cited type(4)

1.	胡婷婷，曹成瑛，赵文兴，王生元，何莹. 基于冠状动脉CT血管造影的血流储备分数对高原高海拔环境下冠心病的诊断价值. 现代生物医学进展. 2024(11): 2173-2176+2191 .
2.	徐磊，尹浩，黄飞，赵渊，和旭，何永福. 分析亚高原地区人群发生冠状动脉性心脏病的影响因素. 大医生. 2024(16): 99-102 .
3.	赵志伟，邢志政，孙晨格，崔梁瑜，任聪，李玉坤，罗玮伯，吕诚，韩学杰，刘大胜. 高原地区冠心病的中医辨证与证素分布规律研究. 中医药导报. 2024(10): 96-102 .
4.	刘斌，张敏，李显刚，林仁华，杨洁，张亮，何跃，王显昆，潘娅楠. 边疆少数民族彝族人群rs1333049单核苷酸多态性与冠心病的联系. 检验医学与临床. 2023(02): 160-165 .

Other cited types(0)

Get Citation

PDF

XML

Article views (193) PDF downloads (12) Cited by(4)

Turn off MathJax

Article Contents

Abstract

References

Influencing factors of false negative of T-cell spot test for tuberculosis infection

Abstract

References

Related Articles

Cited by

Periodical cited type(4)

Other cited types(0)

Catalog

Influencing factors of false negative of T-cell spot test for tuberculosis infection

Abstract

References

Related Articles

Cited by

Periodical cited type(4)

Other cited types(0)

Catalog

Export File

Citation

Format

Content