Effects of human adaptor protein LNK on proliferation and invasion ability of non-small cell lung cancer cells

Objective To investigate the effect of human adaptor protein LNK (hLNK) on the occurrence and development of non-small cell lung cancer (NSCLC).

Methods Human NSCLC cell line H1299-hLNK with stable overexpression of hLNK and control cell line H1299-pCDH were constructed. Western blot was used to verify the expression level of hLNK in the above stable cell lines; the effect of hLNK overexpression on the proliferation of H1299 cells was analyzed by plate cloning; the effect of hLNK overexpression on the invasion ability of H1299 cells was analyzed by scratch assay; the typical marker proteins of epithelial interstitial transition (EMT) in cells were analyzed.

Results Human NSCLC cell line H1299-hLNK overexpressed with hLNK was successfully constructed. Compared with control cells H1299-pCDH, hLNK overexpression significantly inhibited the proliferation and migration of H1299 cells, and the EMT level of H1299 cells was significantly inhibited (P < 0.05).

Conclusion The hLNK can inhibit the proliferation and migration of cancer cells mainly by down-regulating the EMT level of NSCLC cell H1299.