Polyketide compounds in the liquid culture of endophyte <i>Alternaria</i> sp.QDFB-2 from <i>Ficus carica</i> L.

SUN Li; WANG Shasha; CHEN Chunying; ZHAO Xue; WANG Fengwu; SHEN Li

doi:10.7619/jcmp.20231295

Journal of Clinical Medicine in Practice > 2023 > 27(15): 93-97, 103. > DOI: 10.7619/jcmp.20231295

SUN Li, WANG Shasha, CHEN Chunying, ZHAO Xue, WANG Fengwu, SHEN Li. Polyketide compounds in the liquid culture of endophyte Alternaria sp.QDFB-2 from Ficus carica L.[J]. Journal of Clinical Medicine in Practice, 2023, 27(15): 93-97, 103. DOI: 10.7619/jcmp.20231295

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Polyketide compounds in the liquid culture of endophyte Alternaria sp.QDFB-2 from Ficus carica L.

1.
Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001
2.
Jiangsu Key Laboratoryof Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of SenileDiseases, Yangzhou, Jiangsu, 225001
3.
College of Food Science and Engineering of Qingdao Agricultural University, Qingdao, Shandong, 266109

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Received Date: April 22, 2023
Revised Date: May 30, 2023
Available Online: September 03, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the polyketide compounds in the liquid culture of endophyte Alternaria sp.QDFB-2 from Ficus carica L. and evaluate their in vitro cytotoxicity.
Methods
The chemical constituents in the fermentation broth of Alternaria sp.QDFB-2 were fractionated and purified through multiple chromatographic methods such as silica gel column, Sephadex LH-20 gel column, ODS reserved phase columnand high performance liquid chromatography. Then, structures of obtained compounds were determined by modern spectroscopy methods including nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HR-MS) and comparison with literature. Finally, the cytotoxicity of these compounds was evaluated by Methyl Thiazolyl Tetrazolium (MTT) method.
Results
Seven polyketide compounds were obtained from the crude extract of liquid culturefrom Alternaria sp.QDFB-2 and identified as 7-hydroxy-2, 5-dimethyl-4H-1-benzopyran-4-one (1), 1-deoxyrubralactone (2), alternariol-9-O-monomethyl ether (3), alternariol (4), altenuisol (5), stemphyperylenol (6) and altertoxin Ⅰ (7), respectively. The in vitro cytotoxicity assay showed that compound 4 had significant inhibition activity against human cervical carcinoma cell line HeLa and human hepatocellular carcinoma cell line SMMC-7721, with half-maximal inhibitory concentration (IC₅₀) values of 13.43 and 50.47 μg/mL, respectively.
Conclusion
Alternaria sp.QDFB-2 could produce abundant polyketide compounds, which is worthy of further research in developing drugs from microorganism.
- Ficus carica L.,
- endophyte,
- Alternaria sp.,
- secondary metabolite,
- polyketide compound,
- cytotoxicity

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References

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Polyketide compounds in the liquid culture of endophyte Alternaria sp.QDFB-2 from Ficus carica L.

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