Analysis of the chemical constituents in fermentation broth of <i>Myceliophthora lutea</i> ML-1 derived from <i>Concha ostreae</i> based on liquid chromatography tandem mass spectrometry molecular networking

CHEN Chunying; TAO Chao; DING Muzi; WANG Fengwu; ZHU Xiaoli; SHEN Li

doi:10.7619/jcmp.20231458

Journal of Clinical Medicine in Practice > 2023 > 27(20): 105-112, 117. > DOI: 10.7619/jcmp.20231458

CHEN Chunying, TAO Chao, DING Muzi, WANG Fengwu, ZHU Xiaoli, SHEN Li. Analysis of the chemical constituents in fermentation broth of Myceliophthora lutea ML-1 derived from Concha ostreae based on liquid chromatography tandem mass spectrometry molecular networking[J]. Journal of Clinical Medicine in Practice, 2023, 27(20): 105-112, 117. DOI: 10.7619/jcmp.20231458

Citation:

PDF (3423 KB)

Analysis of the chemical constituents in fermentation broth of Myceliophthora lutea ML-1 derived from Concha ostreae based on liquid chromatography tandem mass spectrometry molecular networking

CHEN Chunying^{1, 2},
TAO Chao¹,
DING Muzi¹,
WANG Fengwu³,
ZHU Xiaoli¹,
SHEN Li^{1, 2, ,}

1.
College of Medicine of Yangzhou University, Yangzhou, Jiangsu, 225009
2.
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu, 225009
3.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shangdong, 266109

More Information

Received Date: May 05, 2023
Revised Date: July 06, 2023
Available Online: November 05, 2023

Graphical Abstract

Abstract

Abstract

Objective
To analyze the chemical constituents in the fermentation broth of the symbiotic fungus Myceliophthora lutea (M.lutea) ML-1 derived from Concha ostreae through liquid chromatography tandem mass spectrometry (LC-MS/MS)-based molecular networking so as to discover the secondary metabolite profile of this strain.
Methods
M.lutea ML-1 was cultured in fungal medium number 1. The LC-MS/MS raw data was converted to. mzXML file by MSconvert software, and then uploaded to Global Natural Products Social Molecular Networking (GNPS) platform to establish the classical molecular networking (CMN) for identification of the chemical constituents and prediction of structure. Moreover, LC-MS/MS raw data were pre-processed by MZmine 2 software, and also uploaded to GNPS to create feature-based molecular networking (FBMN). Finally, CMN and FBMN were imported into Cytoscape software for data visualization.
Results
According to CMN, a total of 49 compounds were identified from the fermentation broth of M.lutea ML-1, including 18 fatty acids, 8 anthraquinones, 12 cyclic dipeptides and 11 other compounds, in which there might be two new anthraquinone compounds. The relative quantitative analysis of chemical composition of fermentation broth based on FBMN had identified 3 compounds.
Conclusion
The fermentation broth of M.lutea ML-1 cultured in fungal medium number 1 is rich in chemical constituents, which contains many highly potential active compounds. The results provide ideas for guided isolation of the chemical constituents and optimization of fermentation conditions for the strain in the future.

FullText(HTML)

References (20)

References

[1]	ANCHEEVA E, DALETOS G, PROKSCH P. Bioactive secondary metabolites from endophytic fungi[J]. Curr Med Chem, 2020, 27(11): 1836-1854. doi: 10.2174/0929867326666190916144709
[2]	CHEN J N, XU L, ZHOU Y R, et al. Natural products from actinomycetes associated with marine organisms[J]. Mar Drugs, 2021, 19(11): 629. doi: 10.3390/md19110629
[3]	CHEN S H, CAI R L, LIU Z M, et al. Secondary metabolites from mangrove-associated fungi: source, chemistry and bioactivities[J]. Nat Prod Rep, 2022, 39(3): 560-595. doi: 10.1039/D1NP00041A
[4]	SABDONO A, RADJASA O. Microbial symbionts in marine sponges: marine natural product factory[J]. J Coastal Dev, 2008, 11(2): 57-61. http://www.xueshufan.com/publication/1550609841
[5]	MARIN-FELIX Y, STCHIGEL A M, MILLER A N, et al. A re-evaluation of the genus Myceliophthora (Sordariales, Ascomycota): its segregation into four Genera and description of Corynascus fumimontanus sp. nov[J]. Mycologia, 2015, 107(3): 619-632. doi: 10.3852/14-228
[6]	DE OLIVEIRA SIMÕES L C, DA SILVA R R, DE OLIVEIRA NASCIMENTO C E, et al. Purification and physicochemical characterization of a novel thermostable xylanase secreted by the fungus Myceliophthora heterothallica F. 2.1.4[J]. Appl Biochem Biotechnol, 2019, 188(4): 991-1008. doi: 10.1007/s12010-019-02973-8
[7]	YANG Y J, LIU Y, LIU D D, et al. Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi[J]. Appl Microbiol Biotechnol, 2022, 106(2): 713-727. doi: 10.1007/s00253-021-11733-w
[8]	LI N, LIU Y, LIU D F, et al. MtTRC-1, a novel transcription factor, regulates cellulase production via directly modulating the genes expression of the Mthac-1 and Mtcbh-1 in Myceliophthora thermophila[J]. Appl Environ Microbiol, 2022, 88(19): e0126322. doi: 10.1128/aem.01263-22
[9]	董玉洁, 朴美子, 王懋存, 等. 长岛海洋生物内生真菌的分离及其抗老年痴呆活性筛选[J]. 中国食品学报, 2012, 12(4): 43-47. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGSP201204015.htm
[10]	陶超. 牡蛎共生真菌Myceliophthora sp. ML-1发酵液的化学成分研究[D]. 扬州: 扬州大学, 2022.
[11]	NOTHIAS L F, PETRAS D, SCHMID R, et al. Feature-based molecular networking in the GNPS analysis environment[J]. Nat Methods, 2020, 17(9): 905-908. doi: 10.1038/s41592-020-0933-6
[12]	马小翔, 刘亚月, 聂影影, 等. 基于质谱的分子网络分析化学调控对土曲霉C23-3次生代谢产物及生物活性的影响[J]. 生物技术通报, 2021, 37(8): 95-110. https://www.cnki.com.cn/Article/CJFDTOTAL-SWJT202108010.htm
[13]	廖勤俭, 安明哲, 李杨华, 等. 酒糟黄水中二肽和环二肽的研究[J]. 酿酒科技, 2018(10): 17-23. https://www.cnki.com.cn/Article/CJFDTOTAL-NJKJ201810001.htm
[14]	邓霞玲, 练顺才, 谢正敏, 等. 白酒中环二肽成分的分析研究[J]. 酿酒科技, 2017(9): 27-32. https://www.cnki.com.cn/Article/CJFDTOTAL-NJKJ201709003.htm
[15]	STARK T, HOFMANN T. Structures, sensory activity, and dose/response functions of 2, 5-diketopiperazines in roasted cocoa nibs (Theobroma cacao)[J]. J Agric Food Chem, 2005, 53(18): 7222-7231. doi: 10.1021/jf051313m
[16]	ZHANG Y, WU W P, HU D M, et al. A new thermophilic species of Myceliophthora from China[J]. Mycol Prog, 2014, 13(1): 165-170. doi: 10.1007/s11557-013-0904-8
[17]	GAO Y L, ZHANG M L, WANG X, et al. Isolation and characterization of a new cytotoxic polyketide-amino acid hybrid from Thermothelomyces thermophilus ATCC 42464[J]. Nat Prod Res, 2021, 35(11): 1792-1798. doi: 10.1080/14786419.2019.1641810
[18]	NOZAWA O, OKAZAKI T, SAKAI N, et al. A novel bioactive delta lactone FD-211. Taxonomy, isolation and characterization[J]. J Antibiot, 1995, 48(2): 113-118. doi: 10.7164/antibiotics.48.113
[19]	NOZAWA O, OKAZAKI T, MORIMOTO S, et al. Waol B, a new trihydrofuran derivative with cytocidal activity, isolated from Myceliophthora lutea[J]. J Antibiot, 2000, 53(11): 1296-1300.
[20]	SMETANINA O F, YURCHENKO A N, KALINOVSKII A I, et al. Biologically active metabolites from the marine isolate of the fungus Myceliophthora lutea[J]. Chem Nat Compd, 2011, 47(3): 385-390.

Cited By

Get Citation

PDF

XML

Article views (165) PDF downloads (24)

Turn off MathJax

Article Contents

Abstract

References

Analysis of the chemical constituents in fermentation broth of Myceliophthora lutea ML-1 derived from Concha ostreae based on liquid chromatography tandem mass spectrometry molecular networking

Abstract

References

Catalog

Export File

Citation

Format

Content