Role of basic leucine zipper and W2 domains 2 on the biological behaviors of endometrial cancer cells and its mechanism

TANG Bin; CAI Wen; XU Shanshan; JIANG Zhenghui; WEI Yalan; JIANG Feiyun

doi:10.7619/jcmp.20231643

Journal of Clinical Medicine in Practice > 2023 > 27(18): 18-25. > DOI: 10.7619/jcmp.20231643

TANG Bin, CAI Wen, XU Shanshan, JIANG Zhenghui, WEI Yalan, JIANG Feiyun. Role of basic leucine zipper and W2 domains 2 on the biological behaviors of endometrial cancer cells and its mechanism[J]. Journal of Clinical Medicine in Practice, 2023, 27(18): 18-25. DOI: 10.7619/jcmp.20231643

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Role of basic leucine zipper and W2 domains 2 on the biological behaviors of endometrial cancer cells and its mechanism

Department of Gynecology, East China Normal University Affiliated Wuhu Hospital, the Second People's Hospital of Wuhu City in Anhui Province, Wuhu, Anhui, 241000

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Received Date: May 22, 2023
Revised Date: June 25, 2023
Available Online: October 08, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the regulatory effects of basic leucine zipper and W2 domains 2 (BZW2) on proliferation, invasion, migration and cell cycle of endometrial cancer cells and its potential action mechanism.
Methods
Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expression of BZW2 in human endometrial stromal cell line ESC and human endometrial cancer cells (Ishikawa, KLE, RL95-2, HEC-1-A). BZW2 interference plasmid was transfected into HEC-1-A cells and treated with Wnt/β-catenin signaling pathway agonist SKL2001. CCK-8, clone formation assay, scratch assay and Transwell assay were used to detect cell activity, colony formation, migration and invasion ability, respectively; the cell cycle was detected by flow cytometry; the concentration of BZW2 in Wnt signaling pathway was analyzed by GSEA; the levels of proliferation, metastasis, cycle and Wnt/β-catenin pathway-related proteins were analyzed by Western blot.
Results
The expression of BZW2 was up-regulated in endometrial cancer cells, and interference with BZW2 inhibited the proliferation, migration and invasion of endometrial cancer cells, and induced G₀/G₁ cell arrest (P < 0.001). BZW2 knockdown inactivated the Wnt/β-catenin pathway (P < 0.001), and SKL2001 partially reversed the regulatory effects of BZW2 loss on the proliferation, migration, invasion and cell cycle of HEP-1-A cells (P < 0.001).
Conclusion
Down-regulation of BZW2 can inhibit the proliferation, invasion and migration of endometrial cancer cells, and promote G₀/G₁ cell arrest, which may be related to the inactivation of Wnt/β-catenin signaling pathway. BZW2 may be a new target for the treatment of endometrial cancer.
- endometrial cancer,
- basic leucine zipper and W2 domains 2,
- cell cycle,
- migration,
- invasion,
- Wnt/β-catenin signaling pathway

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References (18)

References

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Role of basic leucine zipper and W2 domains 2 on the biological behaviors of endometrial cancer cells and its mechanism