Expression of programmed death receptor ligand 1 in B cells and B cell subsets of patients with type 1 diabetes mellitus

LI Sicheng; GUO Heming; HUANG Yun; LIU Cuiping; FANG Chen; HU Ji

doi:10.7619/jcmp.20231932

Journal of Clinical Medicine in Practice > 2023 > 27(20): 131-136, 141. > DOI: 10.7619/jcmp.20231932

LI Sicheng, GUO Heming, HUANG Yun, LIU Cuiping, FANG Chen, HU Ji. Expression of programmed death receptor ligand 1 in B cells and B cell subsets of patients with type 1 diabetes mellitus[J]. Journal of Clinical Medicine in Practice, 2023, 27(20): 131-136, 141. DOI: 10.7619/jcmp.20231932

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Expression of programmed death receptor ligand 1 in B cells and B cell subsets of patients with type 1 diabetes mellitus

1.
Department of Endocrinology, the Second Hospital Affiliated to Soochow University, Suzhou, Jiangsu, 215004
2.
Jiangsu Institute of Clinical Immunology of the First Hospital Affiliated to Soochow University, Jiangsu Key Laboratory of Clinical Immunology, Suzhou, Jiangsu, 215006

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Received Date: June 14, 2023
Revised Date: October 09, 2023
Available Online: November 05, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the expression of programmed death receptor ligand 1 (PD-L1) in circulating B cells of patients with type 1 diabetes mellitus (T1DM).
Methods
Peripheral blood samples were collected from healthy controls (n=25) and T1DM patients (n=25). The expression of PD-L1 on B cells and their surfaces was detected by flow cytometry.
Results
There was no significant difference in the frequency of B cell subsets between the T1DM patients and healthy controls (P>0.05). Compared with healthy controls, the expression of PD-L1 on CD19⁺ cells, CD19⁺CD27⁺ cells and CD19⁺CD27⁺ cells in the T1DM patients was significantly decreased (P < 0.05). There was no significant difference in the expression of PD-L1 in B10 cells, marginal zone B cells (MZB) and follicular B cells (FoB) between healthy controls and T1DM patients (P>0.05). The expression of PD-L1 on transitional 2-marginal zone precursor B cells (T2-MZP) cells in the T1DM patients was lower than that in the healthy controls (P < 0.05).
Conclusion
The PD-L1 may play a protective role in the pathogenesis of T1DM. B cells with high expression of PD-L1 may provide a new strategy for the treatment of patients with autoimmune diabetes.
- type 1 diabetes mellitus,
- programmed death receptor ligand 1,
- B cells,
- flow cytometry

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References (24)

References

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Expression of programmed death receptor ligand 1 in B cells and B cell subsets of patients with type 1 diabetes mellitus