Effect of lycopene on oxidized low-density lipoprotein induced apoptosis of cerebral vascular endothelial cells by regulating the RAS homologous gene family member A/Rho-related kinase signaling pathway

ZHANG Junchen; XU Wuhua; ZHONG Li

doi:10.7619/jcmp.20232790

Journal of Clinical Medicine in Practice > 2023 > 27(22): 44-49. > DOI: 10.7619/jcmp.20232790

ZHANG Junchen, XU Wuhua, ZHONG Li. Effect of lycopene on oxidized low-density lipoprotein induced apoptosis of cerebral vascular endothelial cells by regulating the RAS homologous gene family member A/Rho-related kinase signaling pathway[J]. Journal of Clinical Medicine in Practice, 2023, 27(22): 44-49. DOI: 10.7619/jcmp.20232790

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Effect of lycopene on oxidized low-density lipoprotein induced apoptosis of cerebral vascular endothelial cells by regulating the RAS homologous gene family member A/Rho-related kinase signaling pathway

1.
Department of Neurology, Zhongjiang County People's Hospital in Sichuan Province, Deyang, Sichuan, 618100
2.
Department of Rehabilitation Medicine (the Second Division of Department of Neurology), Institute of Clinical Pathological Nutrition, Guangzhou Red Cross Hospital Affiliated to Jinan University, Guangzhou, Guangdong, 510220
3.
Yibin City Second People's Hospital of Sichuan Province, Yibin, Sichuan, 644002

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Received Date: September 03, 2023
Revised Date: October 15, 2023
Available Online: December 05, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of lycopene (LYC) on the oxidized low-density lipoprotein (ox-LDL) induced apoptosis of cerebral vascular endothelial cells (EC) by regulating the RAS homologous gene family member A (RhoA)/Rho-related kinase (ROCK) signaling pathway.
Methods
Human brain microvascular endothelial cells (HBMECs) without any treatments were designed as NC group; the HBMECs treated with ox-LDL were divided into ox-LDL group, LYC group (HBMECs treated with 0.5 μmol/L LYC), lysophosphatidic acid (LPA) group (HBMECs treated with 5 μmol/L RhoA/ROCK signaling pathway activator LPA), and LYC+LPA group (HBMECs treated with 0.5 μmol/L LYC and 5 μmol/L LPA); after 24 hours of treatment, they were used for subsequent experiments. Enzyme-linked immunosorbent assay (ELISA) was used to detect the cytokine levels of HBMECs; CCK-8 method was used to detect the proliferation of HBMECs; flow cytometry was used to detect the apoptosis rate of HBMECs; the Western blot was used to detect the expression levels of platelet endothelial cell adhesion molecule-1 (CD31), smooth muscle (SM) 22α, BCL-2 associated X protein (Bax), B-cell lymphoma/leukemia 2 protein (Bcl-2), cleaved-Caspase-3, and RhoA/ROCK pathway related proteins.
Results
Compared with the NC group, the levels of monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6), vascular endothelial cell adhesion molecule-1 (VCAM-1), apoptosis rate of HBMECs, the Bax, cleaved-Caspase-3, SM22α, RhoA and ROCK1 proteins in the ox-LDL group increased significantly, while the OD value and the levels of Bcl-2 and CD31 proteins decreased significantly (P < 0.05); compared with the ox-LDL group, the levels of MCP-1, IL-6, VCAM-1, apoptosis rate of HBMECs, the Bax, cleaved-Caspase-3, SM22α, RhoA, and ROCK1 proteins in the LYC group decreased significantly, while the OD value and the levels of Bcl-2 and CD31 proteins increased significantly (P < 0.05), but the trends in the LPA group were opposite; LPA weakened the improvement effect of LYC on apoptosis of HBMECs induced by ox-LDL.
Conclusion
LYC may reduce apoptosis of HBMECs by inhibiting the RhoA/ROCK signaling pathway.

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References (29)

References

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Effect of lycopene on oxidized low-density lipoprotein induced apoptosis of cerebral vascular endothelial cells by regulating the RAS homologous gene family member A/Rho-related kinase signaling pathway