Effect of silencing E26 transformation-specific sequence 4 on proliferation and migration of colon cancer cells by inhibiting nuclear factor-κB signaling pathway

XU Hexi; SONG Hongqi; LIU Dianwen; LIU Shiju; YANG Huiju

doi:10.7619/jcmp.20243267

Journal of Clinical Medicine in Practice > 2025 > 29(2): 38-41,47. > DOI: 10.7619/jcmp.20243267

XU Hexi, SONG Hongqi, LIU Dianwen, LIU Shiju, YANG Huiju. Effect of silencing E26 transformation-specific sequence 4 on proliferation and migration of colon cancer cells by inhibiting nuclear factor-κB signaling pathway[J]. Journal of Clinical Medicine in Practice, 2025, 29(2): 38-41,47. DOI: 10.7619/jcmp.20243267

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Effect of silencing E26 transformation-specific sequence 4 on proliferation and migration of colon cancer cells by inhibiting nuclear factor-κB signaling pathway

1.
Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, 450046
2.
Anorectal Department, the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, 450046

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Received Date: July 30, 2024
Revised Date: October 27, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the mechanism of E26 transformation-specific sequence 4 (ETV4) affecting the proliferation and migration of colon cancer cells through the nuclear factor-κB (NF-κB) signaling pathway.
Methods
The expression level of ETV4 in normal colon tissues and cancer tissues was analyzed by the user-friendly interactive cancer transcriptome data analysis resource (UALCAN) database. Reverse transcription quantitative polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression level of ETV4 in normal intestinal epithelial cells and colon cancer cell lines. After silencing ETV4 in SW480 cells, qRT-PCR and Western blot were performed to detect the expression of ETV4 to assess transfection efficiency; colony formation and Transwell assays were conducted to explore the effects of ETV4 silencing on the proliferation and migration of colon cancer cells; the Western blot was used to detect the effects of ETV4 silencing on the protein expression of protein 65 (p65) and phosphorylated protein 65 (p-p65) in the NF-κB pathway.
Results
The UALCAN database analysis revealed high expression of ETV4 in colon cancer tissues. The qRT-PCR and Western blot showed that ETV4 expression was significantly higher in the colon cancer cell lines SW480, Lovo, Caco-2, and SW620 than in normal intestinal epithelial cells HIEC-6, with the highest expression in SW480 cells (P < 0.001). Colony formation and Transwell assay results indicated that silencing ETV4 significantly inhibited the proliferation and migration of colon cancer SW480 cells (P < 0.001). Western blot results showed that silencing ETV4 significantly inhibited the expression of p-p65 protein in the cells (P < 0.001).
Conclusion
Silencing ETV4 may inhibit the activation of the NF-κB signaling pathway, thereby inhibiting the proliferation and migration of colon cancer cells.
- E26 transformation-specific sequence 4,
- nuclear factor-κB,
- colon cancer,
- cell proliferation,
- cell migration

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References

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Effect of silencing E26 transformation-specific sequence 4 on proliferation and migration of colon cancer cells by inhibiting nuclear factor-κB signaling pathway

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