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外周血辅助性T细胞17与调节性T细胞的平衡与白癜风临床特征的相关性

Relationship between clinical features of vitiligo and balance of T helper cell 17 and regulatory T cell in peripheral blood

    摘要
  • 摘要:
      目的   分析白癜风患者外周血辅助性T细胞17(Th17)与调节性T细胞(Treg)的平衡与临床特征的关系。
      方法  选取白癜风患者109例，其中进展期患者56例，稳定期患者53例，另选取同期本院健康体检者59例为对照组。采用流式细胞术检测3组受试者外周血Th17、Treg细胞数量; 采用酶联免疫吸附法(ELISA)检测3组受试者白介素-17(IL-17)、叉头盒蛋白P3(Foxp3)水平; 采用Pearson法分析白癜风患者Th17、Treg细胞及IL-17、Foxp3的关系。
      结果  对照组、稳定期组、进展期组Th17细胞含量、Th17/Treg、IL-17水平逐渐升高, Treg细胞含量、Foxp3水平逐渐降低，差异有统计学意义(P < 0.05)。随着白癜风皮损面积的增加, Th17细胞含量、Th17/Treg、IL-17水平逐渐升高, Treg细胞含量、Foxp3水平逐渐降低，差异有统计学意义(P < 0.05)。白癜风患者Th17细胞与Treg细胞、IL-17与Foxp3均呈显著负相关(r=-0.449、-0.497, P < 0.05)。
      结论  Th17/Treg细胞平衡可能与白癜风的发生及临床特征有关。

     

    Abstract:
      Objective  To analyze the correlation between progression of disease and balance of T helper cell 17 (Th17) and regulatory T cell (Treg) in peripheral blood of patients with vitiligo.
      Methods  A total of 109 patients with vitiligo were selected, including 56 patients in progressive stage and 53 patients in stable stage. At the same time, 59 healthy people with physical examinations were selected as control group. The number of Th17 and Treg cells in peripheral blood of the three groups was detected by flow cytometry. The levels of interleukin-17 (IL-17) and forkhead-box-protein P3 (Foxp3) were detected by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the relationships between Th17, Treg cells and IL-17, Foxp3 in patients with vitiligo.
      Results   With the progress of the disease, the Th17 cell content, Th17/Treg and IL-17 levels in the control group, stable group and advanced group increased gradually, while the Treg cell content and Foxp3 level decreased gradually (P < 0.05). With the increase of vitiligo lesion area, Th17 cell content, Th17/Treg and IL-17 levels gradually increased, while Treg cell content and Foxp3 level gradually decreased (P < 0.05). There were significant negative correlations between Th17 cells and Treg cells, IL-17 and Foxp3 in vitiligo patients (r=-0.449, -0.497, P < 0.05).
      Conclusion   Conclusion Th17/Treg cell balance may be related to the occurrence and clinical features of vitiligo.

     

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