Serum and placental levels of asymmetric dimethylarginine, Annexin V and Survivin in preeclamptic patients and their significance
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摘要:目的 探讨不对称二甲基精氨酸(ADMA)、膜联蛋白Ⅴ(AnnexinⅤ)、生存蛋白(Survivin)在子痫前期患者血清及胎盘组织中的水平及意义。方法 选取产科住院的子痫前期患者60例为子痫前期组，另外选取同期产科住院的健康产妇60例为健康产妇组。分析ADMA、AnnexinⅤ、Survivin在子痫前期患者血清及胎盘组织中的表达水平及相关性。结果 子痫前期组产妇血清及胎盘组织中的ADMA表达水平高于健康产妇组, AnnexinⅤ、Survivin表达水平低于健康产妇组，差异有统计学意义(P < 0.05)。早发型子痫前期患者血清及胎盘组织ADMA表达水平高于晚发型子痫前期患者, AnnexinⅤ、Survivin表达水平低于晚发型子痫前期患者，差异有统计学意义(P < 0.05)。轻度子痫前期患者血清及胎盘组织ADMA表达水平低于重度子痫前期患者, AnnexinⅤ、Survivin表达水平高于重度子痫前期患者，差异有统计学意义(P < 0.05)。ADMA与AnnexinⅤ、Survivin呈负相关(r=-0.481、-0.683, P=0.001、0.001), AnnexinⅤ与Survivin呈正相关(r=0.495, P=0.001)。结论 ADMA、AnnexinⅤ、Survivin在子痫前期患者血清及胎盘组织中呈特异性表达，且与病情轻重关系密切，为临床子痫前期诊治提供了新的理论参考。Abstract:Objective To explore levels and significance of asymmetric dimethylarginine (ADMA), Annexin V, and Survivin in the serum and placental tissues of preeclamptic patients.Methods A total of 60 hospitalized preeclampsia patients in department of obstetrics were selected as preeclampsia group, and another 60 healthy pregnant women in department of obstetrics during the same period were selected as healthy pregnant group. The expression levels of ADMA, Annexin V and Survivin in serum and placenta samples from preeclamptic patients were analyzed, and their correlations were analyzed.Results The ADMA expression levels in the maternal serum and placental tissues in the preeclampsia group were higher than those in healthy pregnant women, and the expression levels of Annexin V and Survivin were lower than those in healthy pregnant group (P < 0.05). ADMA expression levels in serum and placental tissues of early-onset preeclampsia patients were higher than those of late-onset preeclampsia patients, and Annexin V and Survivin expression levels were lower than those of late-onset preeclampsia patients (P < 0.05). ADMA expression levels in serum and placental tissues of mild preeclampsia patients were lower than those of severe preeclampsia patients, and Annexin V and Survivin expression levels were higher than those of severe preeclampsia patients (P < 0.05). There were negative correlations between ADMA and Annexin V (r=-0.481, P=0.001), between ADMA and Survivin (r=-0.683, P=0.001). ADMA and Survivin was positively correlated (r=0.495, P=0.001).Conclusion ADMA, Annexin V and Survivin are specifically expressed in the serum and placental tissue of patients with preeclampsia, and they have close relationships with the severity of the disease, which provide a new theoretical reference for the diagnosis and treatment of preeclampsia in clinical practice.
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Keywords:
- asymmetric dimethylarginine /
- Annexin V /
- Survivin /
- preeclampsia /
- placenta
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图 1 ADMA、AnnexinⅤ、Survivin之间相关性分析
A: ADMA与AnnexinⅤ的相关性; B: ADMA与Survivin的相关性; C: AnnexinⅤ与Survivin的相关性。
表 1 2组产妇血清及胎盘组织中的ADMA、AnnexinⅤ、Survivin表达水平比较(x±s)
组别 n ADMA/(μmol/L) AnnexinⅤ/(ng/mL) Survivin/(pg/mL) 血清 胎盘组织 血清 胎盘组织 血清 胎盘组织 健康产妇组 60 1.28±0.19 1.86±0.18 1.36±0.21 1.42±0.15 352.96±35.23 32.16±2.52 子痫前期组 60 1.89±0.21* 2.26±0.20* 0.65±0.11* 0.76±0.08* 205.17±23.66* 3.98±0.65* ADMA: 不对称二甲基精氨酸; AnnexinⅤ: 膜联蛋白V; Survivin: 生存蛋白。与健康产妇组比较, *P < 0.05。 
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表 2 早发型与晚发型子痫前期患者血清及胎盘组织ADMA、AnnexinⅤ、Survivin表达水平比较(x±s)
子痫类型 n ADMA/(μmol/L) AnnexinⅤ/(ng/mL) Survivin/(pg/mL) 血清 胎盘组织 血清 胎盘组织 血清 胎盘组织 早发型 24 2.02±0.14* 2.48±0.17* 0.50±0.11* 0.61±0.07* 128.53±22.65* 1.31±0.03* 晚发型 36 1.76±0.13 2.12±0.11 0.79±0.12 0.92±0.09 282.36±42.17 5.37±0.42 ADMA: 不对称二甲基精氨酸; AnnexinⅤ: 膜联蛋白V; Survivin: 生存蛋白。与晚发型子痫患者比较, *P < 0.05。
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表 3 轻度与重度子痫前期患者血清及胎盘组织ADMA、AnnexinⅤ、Survivin表达水平比较(x±s)
子痫类型 n ADMA/(μmol/L) AnnexinⅤ/(ng/mL) Survivin/(pg/mL) 血清 胎盘组织 血清 胎盘组织 血清 胎盘组织 轻度 34 1.55±0.12* 2.05±0.19* 0.85±0.13* 1.08±0.16* 280.45±39.87* 5.48±0.51* 重度 26 2.10±0.14 2.51±0.20 0.47±0.13 0.59±0.07 132.14±21.98 1.29±0.02 ADMA: 不对称二甲基精氨酸; AnnexinⅤ: 膜联蛋白V; Survivin: 生存蛋白。与重度子痫患者比较, *P < 0.05。
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