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胃癌组织中调宁蛋白1和肿瘤泛素异肽酶1的表达与临床病理特征的关系

Relationships of expressions of Calponin 1 and tumor ubiquitin-specific protease 1 with clinical pathological characteristics in gastric cancer tissues

    摘要
  • 摘要: 目的 探讨胃癌组织中调宁蛋白1(Calponin 1)、肿瘤泛素异肽酶1(OTUB1)表达与临床病理特征的关系及预后价值。方法 选取98例胃癌患者为研究对象。采用qPCR检测胃癌组织中Calponin 1 mRNA、OTUB1 mRNA的表达; 采用免疫组织化学方法检测Calponin 1、OTUB1的蛋白表达; 绘制Kaplan-Meier曲线比较不同Calponin 1、OTUB1表达的胃癌患者的生存差异; 采用Cox回归模型筛选胃癌预后的影响因素。结果 与癌旁组织比较,胃癌组织中Calponin 1 mRNA、OTUB1 mRNA表达量较高，差异有统计学意义(P<0.05)。胃癌组织中Calponin 1、OTUB1蛋白阳性表达率为63.27%、65.31%, 高于癌旁组织的6.12%、8.16%, 差异有统计学意义(P<0.001)。TNM分期Ⅲ期、有淋巴结转移的胃癌组织中Calponin 1、OTUB1蛋白表达水平升高，差异有统计学意义(P<0.05)。Calponin 1阳性组3年总生存率为45.16%(28/62), 低于阴性组的83.33%(30/36), 差异有统计学意义(Log-rank χ2=12.990, P<0.001); OTUB1阳性组3年总生存率为45.31%(29/64), 低于阴性组的85.29%(29/34), 差异有统计学意义(Log-rank χ2=14.880, P<0.001)。多因素Cox回归结果显示, Calponin 1阳性、OTUB1阳性、TNM分期Ⅲ期、有淋巴结转移是胃癌患者死亡的危险因素(P<0.001)。结论 胃癌组织中Calponin 1、OTUB1表达上调,与TNM分期、淋巴结转移有关。

     

    Abstract: Objective To investigate the relationships of the expression of Calponin 1 and tumor ubiquitin-specific protease 1 (OTUB1) with clinicopathological features in gastric cancer tissues as well as their prognostic values. Methods A total of 98 patients with gastric cancer were enrolled in this study. The qPCR was used to detect the expression levels of Calponin 1 mRNA and OTUB1 mRNA in gastric cancer tissues; the immunohistochemical staining was performed to detect the protein expression levels of Calponin 1 and OTUB1; the Kaplan-Meier curve was plotted to compare the survival differences among gastric cancer patients with different expression levels of Calponin 1 and OTUB1; the Cox regression model was used to screen the influencing factors of prognosis in gastric cancer. Results Compared with adjacent tissues, the expression levels of Calponin 1 mRNA and OTUB1 mRNA in gastric cancer tissues were significantly higher (P<0.05). The positive expression rates of Calponin 1 and OTUB1 protein in gastric cancer tissues were 63.27% and 65.31% respectively, which were significantly higher than 6.12% and 8.16% in adjacent tissues (P<0.001). The expression levels of Calponin 1 and OTUB1 proteins were increased significantly in gastric cancer tissues of cases with stage Ⅲ of TNM staging and lymph node metastasis (P<0.05). The 3-year overall survival rate was 45.16% (28/62) in the Calponin 1-positive group, which was significantly lower than 83.33% (30/36) in the Calponin 1-negative group (Log-rank χ2=12.990, P<0.001). The 3-year overall survival rate was 45.31% (29/64) in the OTUB1-positive group, which was significantly lower than 85.29% (29/34) in the OTUB1-negative group (Log-rank χ2=14.880, P<0.001). The results of multivariate Cox regression showed that Calponin 1 positivity, OTUB1 positivity, stage Ⅲ of TNM staging, and lymph node metastasis were risk factors for death in gastric cancer patients (P<0.001). Conclusion The expressions of Calponin 1 and OTUB1 are upregulated in gastric cancer tissues and are related to TNM staging and lymph node metastasis.

     

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