YAN Keshi, WANG Weiwei, YU Xin, LU Dahao, DAI Chen, GAO Ju. Single versus multiple administrations of metformin in treatment of rats with ventilator-induced brain injury[J]. Journal of Clinical Medicine in Practice, 2020, 24(3): 33-35. DOI: 10.7619/jcmp.202003010
Citation: YAN Keshi, WANG Weiwei, YU Xin, LU Dahao, DAI Chen, GAO Ju. Single versus multiple administrations of metformin in treatment of rats with ventilator-induced brain injury[J]. Journal of Clinical Medicine in Practice, 2020, 24(3): 33-35. DOI: 10.7619/jcmp.202003010

Single versus multiple administrations of metformin in treatment of rats with ventilator-induced brain injury

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  • Received Date: November 17, 2019
  • Objective To investigate effect of single and multiple administrations of metformin on treatment of rats with ventilator-induced brain injury. Methods A total of 24 healthy adult male Wistar rats were randomly divided into 4 groups, including control group(group C, n=6), mechanical ventilation group(MV group, n=6), mechanical ventilation combined with single-does metformin group(MET group, n=6), and mechanical ventilation with multiple-does metformin group(M-MET group, n=6). Group C retained spontaneous breathing after endotracheal intubation without mechanical ventilation. MV group, MET group and M-MET group were performed mechanical ventilation. Metformin was fed 500 mg/kg in the MET group before the experiment and 500 mg/(kg·d)in the M-MET group for 4 weeks. At the end of mechanical ventilation, all rats were sacrificed for brain tissue to observe pathological changes under light microscope. Interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)of brain tissue homogenate were measured by Enzyme linked immunosorbent assay(ELISA)kits, superoxide dismutase(SOD)and malondialdehyde(MDA)were measured with kits. Results Compared with group C, MV group, MET group and M-MET group had severe pathological damage, the contents of IL-6, TNF-α and MDA significantly increased, while the level of SOD decreased significantly(P < 0.05). Compared with MV group, pathological damage of the MET and - M-MET groups were alleviated, the levels of IL-6, TNF-α and MDA significantly decreased, and the level of SOD significantly increased(P < 0.05). Compared with MET group, the levels of IL-6, TNF-α, MDA and SOD in M-MET group were significantly higher(P < 0.05). Conclusion Metformin can reduce the brain injury induced by mechanical ventilation in rats. The mechanism may be related to the inhibition of inflammatory pathway and oxidative stress, and the effect of single administration is better than that of multiple administration.
  • Quilez M E, Rodríguez-González R, Turon M, et al. Moderate peep after tracheal lipopolysaccharide instillation prevents inflammation and modifies the pattern of brain neuronal activation[J]. Shock, 2015, 44(6): 601-608.
    Quilez M E, Fuster G, Villar J, et al. Injurious mechanical ventilation affects neuronal activation in ventilated rats[J]. Crit care, 2011, 15(3): R124.
    Blanch L, Quintel M. Lung-brain cross talk in the critically ill[J]. Intensive Care Med, 2017, 43(4): 557-559.
    余鑫, 米智华, 高巨. 自噬在机械通气致大鼠脑组织神经元凋亡中的作用[J]. 实用临床医药杂志, 2019, 23(8): 12-15

    , 21.
    Anisimov V N, Berstein L M, Egormin P A, et al. Metformin slows down aging and extends life span of female SHR mice[J]. Cell Cycle, 2008, 7(17): 2769-2773.
    Knowler W C, Barrett-Connor E, Fowler S E, et al. Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin[J]. N Engl J Med, 2002, 346(6): 393-403.
    Ng T P, Feng L, Yap K B, et al. Long-term metformin usage and cognitive function among older adults with diabetes[J]. J Alzheimers Dis, 2014, 41(1): 61-68.
    Guo M, Mi J, Jiang Q M, et al. Metformin may produce antidepressant effects through improvement of cognitive function among depressed patients with diabetes mellitus[J]. Clin Exp Pharmacol Physiol, 2014, 41(9): 650-656.
    Mudgal J, Nampoothiri M, Basu Mallik S, et al. Possible involvement of metformin in downregulation of neuroinflammation and associated behavioural changes in mice[J]. Inflammopharmacology, 2019, 27(5): 941-948.
    Li W J, Chaudhari K, Shetty R, et al. Metformin alters locomotor and cognitive function and brain metabolism in normoglycemic mice[J]. Aging Dis, 2019, 10(5): 949-963.
    Dreyfuss D, Saumon G. Ventilator-induced lung injury: lessons from experimental studies[J]. Am J Respir Crit Care Med, 1998, 157(1): 294-323.
    Turon M, Fernández-Gonzalo S, de Haro C, et al. Mechanisms involved in brain dysfunction in mechanically ventilated critically ill patients: implications and therapeutics[J]. Ann Transl Med, 2018, 6(2): 30.
    Pelosi P, Rocco P R. The lung and the brain: a dangerous cross-talk[J]. Crit Care, 2011, 15(3): 168.
    Liu Y Q, Tang G H, Li Y N, et al. Metformin attenuates blood-brain barrier disruption in mice following middle cerebral artery occlusion[J]. J Neuroinflammation, 2014, 11: 177.
    Tang G M, Yang H Y, Chen J, et al. Metformin ameliorates Sepsis-induced brain injury by inhibiting apoptosis, oxidative stress and neuroinflammation via the PI3K/Akt signaling pathway[J]. Oncotarget, 2017, 8(58): 97977-97989.
    Ye J H, Zhu N, Sun R P, et al. Metformin inhibits chemokine expression through the AMPK/NF-κB signaling pathway[J]. J Interf Cytokine Res, 2018, 38(9): 363-369.
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