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WANG Di, XU Nan, GUO Jiayan, SONG Yue, TANG Mingrui. Berberine inhibits proliferation and promotes apoptosis of melanoma B16 cells by up-regulating programmed cell death 4[J]. Journal of Clinical Medicine in Practice, 2020, 24(11): 82-86. DOI: 10.7619/jcmp.202011023
Citation: WANG Di, XU Nan, GUO Jiayan, SONG Yue, TANG Mingrui. Berberine inhibits proliferation and promotes apoptosis of melanoma B16 cells by up-regulating programmed cell death 4[J]. Journal of Clinical Medicine in Practice, 2020, 24(11): 82-86. DOI: 10.7619/jcmp.202011023
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Berberine inhibits proliferation and promotes apoptosis of melanoma B16 cells by up-regulating programmed cell death 4

  • Department of Plastic Surgery, The First Hospital Affiliated to China Medical University, Shenyang, Liaoning, 110001

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  • Received Date: March 12, 2020
  • Available Online: August 27, 2020
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    • Abstract
  • Objective To explore the effect of berberine on the proliferation and apoptosis of melanoma B16 cells. Methods The CCK-8 method was used to detect cell proliferation viability. Cell cycle was detected by flow cytometry. The programmed cell death 4(PDCD4)gene was knocked down by liposome transfection of siRNA. Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)was used to identify knockout condition. Cell apoptosis was detected by flow cytometry. Expressions of PDCD4, proliferation-associated and apoptosis-associated proteins were detected by Western-blotting. Results Berberine was able to inhibit the proliferation viability of melanoma B16 cells and arrest cell cycle at G0/G1 phase.PDCD4 was the key gene for berberine to inhibit proliferation and promote apoptosis of melanoma B16 cells. Berberine treatment was able to increase the expression levels of PDCD4, Cleaved Caspase-3 and Bcl-2 associated protein X(Bax)as well as decrease expression levels of Ki-67 and Bcl-2. The effect of berberine on Ki-67, Bcl-2, Bax and Cleaved Caspase-3 of melanoma B16 cells was weakened by knocking out PDCD4. Conclusion Berberine can inhibit proliferation and promote apoptosis of melanoma B16 cells by up-regulating PDCD4.
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