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WU Shuangshi, LI Hongda, ZHAO Jiwei, SUN Hao, HU Le, WANG Yongxiang. Research on dexamethasone binding region and related transcription factors of Nogo-A promoter[J]. Journal of Clinical Medicine in Practice, 2020, 24(20): 1-5,14. DOI: 10.7619/jcmp.202020001
Citation: WU Shuangshi, LI Hongda, ZHAO Jiwei, SUN Hao, HU Le, WANG Yongxiang. Research on dexamethasone binding region and related transcription factors of Nogo-A promoter[J]. Journal of Clinical Medicine in Practice, 2020, 24(20): 1-5,14. DOI: 10.7619/jcmp.202020001
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Research on dexamethasone binding region and related transcription factors of Nogo-A promoter

  • Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, 225000

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  • Received Date: August 27, 2020
  • Available Online: December 21, 2020
  • Published Date: November 12, 2020
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    • Abstract
  • Objective To explore the dexamethasone binding region and related transcription factors of Nogo-A promoter. Methods Nogo-A promoters with different lengths were constructed and inserted into pGL3 basic vector, and then was transfected into SGC-7901 cells. The transfected cells were treated with different doses of dexamethasone. The dexamethasone binding region of Nogo-A promoter was explored by fluorescence analysis. Bioinformatics method was used to analyze the transcription factor binding sites of this sequence. Results The dexamethasone binding region of Nogo-A promoter was from -1 218 bp to -833 bp. There were 90 possible transcription factors in the target region. There was no CpG island in Nogo-A promoter region. Conclusion Dexamethasone may regulate Nogo-A by binding transcription factor c-Jun with -1 218 bp to -833 bp upstream of Nogo-A promoter.
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