Research on dexamethasone binding region and related transcription factors of Nogo-A promoter

Objective To explore the dexamethasone binding region and related transcription factors of Nogo-A promoter. Methods Nogo-A promoters with different lengths were constructed and inserted into pGL3 basic vector, and then was transfected into SGC-7901 cells. The transfected cells were treated with different doses of dexamethasone. The dexamethasone binding region of Nogo-A promoter was explored by fluorescence analysis. Bioinformatics method was used to analyze the transcription factor binding sites of this sequence. Results The dexamethasone binding region of Nogo-A promoter was from -1 218 bp to -833 bp. There were 90 possible transcription factors in the target region. There was no CpG island in Nogo-A promoter region. Conclusion Dexamethasone may regulate Nogo-A by binding transcription factor c-Jun with -1 218 bp to -833 bp upstream of Nogo-A promoter.