GU Ting, LIU Xiaoqing, CAO Feng, ZHANG Fengbo. Expression level of miRNA-21 in peripheral blood of children with allergic rhinitis and its clinical significance[J]. Journal of Clinical Medicine in Practice, 2021, 25(16): 13-17, 21. DOI: 10.7619/jcmp.20212536
Citation: GU Ting, LIU Xiaoqing, CAO Feng, ZHANG Fengbo. Expression level of miRNA-21 in peripheral blood of children with allergic rhinitis and its clinical significance[J]. Journal of Clinical Medicine in Practice, 2021, 25(16): 13-17, 21. DOI: 10.7619/jcmp.20212536

Expression level of miRNA-21 in peripheral blood of children with allergic rhinitis and its clinical significance

More Information
  • Received Date: June 17, 2021
  • Available Online: September 12, 2021
  • Published Date: August 27, 2021
  •   Objective   To investigate expression level of serum miRNA-21 in children with allergic rhinitis(AR).
      Methods   A total of 108 AR children and 45 healthy children were respectively included in AR group and control group. All children with AR were treated with Fluticasone Propionate Nasal Spray combined with Montelukast Sodium Chewable Tablets for 4 weeks. The levels of miRNA-21, eosinophil (EOS) count, eosinophil cationic protein (ECP) and total IgE (TIgE) levels were compared between two groups before and after treatment. The correlations between miRNA-21, EOS count, ECP, TIgE and Visual Analogue Scale (VAS) scores before and after treatment in children with AR were analyzed by Pearson correlation analysis. The clinical efficacy of miRNA-21, EOS count, ECP and TIgE in evaluating the response condition after treatment in children with AR was explored by Receiver Operating Characteristic (ROC) curve.
      Results   A total of 84 children (77.78%) with ARacquired significant efficiency after 4 weeks of treatment. The levels of miRNA-21, ECP, TIgE and EOS count in the AR group were significantly higher than after treatment and control group, and the level of miRNA-21 after treatment in the AR group was significantly higher than those of the control group (P < 0.05). The VAS scores of AR children decreased significantly after treatment (P < 0.05). Before treatment, miRNA-21, EOS count and ECP in the children of the AR group showed significantly positive correlations with VAS scores (r=0.568, 0.433, 0.352). However, there were no correlations between miRNA-21, EOS count, ECP and VAS scores in the AR group after treatment. The Area Under the Curves (AUC) of miRNA-21, ECP, EOS counts, TIgE were 0.913, 0.859, 0.694 and 0.688, respectively. The AUC of miRNA-21 was significantly higher than ECP, EOS counts and TIgE, the AUC of ECP was significantly higher than EOS counts and TIgE, the differences were significant(P < 0.05). However, the comparison of AUCs of EOS counts and TIgE showed no significant difference (P>0.05). The AUC of miRNA-21 combined with ECP in evaluating the significant efficiency after 4 weeks of treatment in children with AR was 0.984 (95%CI, 0.863 to 0.995, P < 0.05), and the specificity and sensitivity was 90.52% and 94.75%, respectively.
      Conclusion   The expression of miRNA-21 in children with AR is significantly higher, which can reflect the severity of the disease. Combined detection of miRNA-21 and ECP or miRNA-21 alone both can predict the clinical efficacy of children with AR.
  • [1]
    HINDS D, AGGARWAL B, DU X, et al. Asia Pacific survey of physicians on asthma and allergic rhinitis (ASPAIR): data from China[J]. Chin Med J: Engl, 2019, 132(11): 1264-1271. doi: 10.1097/CM9.0000000000000229
    [2]
    TABATABAIAN F, CASALE T B. Allergic rhinitis management: what′s next[J]. Expert Rev Clin Immunol, 2018, 14(3): 191-196. doi: 10.1080/1744666X.2018.1438270
    [3]
    ZHANG J, WEI B, HU H, et al. The association between differentially expressed micro RNAs in breast cancer cell lines and the micro RNA-205 gene polymorphism in breast cancer tissue[J]. Oncol Lett, 2018, 15(2): 2139-2146. http://smartsearch.nstl.gov.cn/paper_detail.html?id=bede5479f662c4afbd33b3ae51802b93
    [4]
    冯娟, 张秀利, 阳玉萍, 等. 变应性鼻炎患者鼻黏膜微小RNA的表达谱及其意义[J]. 新疆医科大学学报, 2019, 42(10): 1284-1289. doi: 10.3969/j.issn.1009-5551.2019.10.009
    [5]
    LEE H Y, LEE H Y, CHOI J Y, et al. Inhibition of MicroRNA-21 by an antagomir ameliorates allergic inflammation in a mouse model of asthma[J]. Exp Lung Res, 2017, 43 (3): 109-119. doi: 10.1080/01902148.2017.1304465
    [6]
    侯红枝, 辛学东. 变应性鼻炎儿童心理行为学调查及影响因素研究[J]. 河北医药, 2018, 40(19): 3010-3012. doi: 10.3969/j.issn.1002-7386.2018.19.034
    [7]
    黄雨, 张睿欣, 刘运可, 等. 学龄儿童变应性鼻炎和非变应性鼻炎3年后回访的临床特点及发生哮喘情况对比[J]. 实用医学杂志, 2018, 34(9): 1491-1494. doi: 10.3969/j.issn.1006-5725.2018.09.021
    [8]
    中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组、小儿学组, 中华儿科杂志编辑委员会. 儿童变应性鼻炎诊断和治疗指南(2010年, 重庆)[J]. 中华耳鼻咽喉头颈外科杂志, 2011, 46(1): 7-8. https://cdmd.cnki.com.cn/Article/CDMD-10611-1014044856.htm
    [9]
    中华耳鼻咽喉头颈外科杂志编辑委员会, 中华医学会耳鼻咽喉科分会. 变应性鼻炎的诊治原则和推荐方案(2004年, 兰州)[J]. 中国社区医师, 2005, 21(16): 17-18. https://cdmd.cnki.com.cn/Article/CDMD-10730-1017715094.htm
    [10]
    BOUSQUET P J, COMBESCURE C, NEUKIRCH F, et al. Visual analog scales can assess the severity of rhinitis graded according to ARIA guidelines[J]. Allergy, 2007, 62(4): 367-372. doi: 10.1111/j.1398-9995.2006.01276.x
    [11]
    MENG Y, WANG C, ZHANG L. Advances and novel developments in allergic rhinitis[J]. Allergy, 2020, 75(12): 3069-3076. doi: 10.1111/all.14586
    [12]
    JAGGI V, DALAL A, RAMESH B R, et al. Coexistence of allergic rhinitis and asthma in Indian patients: The CARAS survey[J]. Lung India, 2019, 36(5): 411-416. doi: 10.4103/lungindia.lungindia_491_18
    [13]
    LU Z, CHEN L, XU S, et al. Allergic disorders and risk of depression: a systematic review and meta-analysis of 51 large-scale studies[J]. Ann Allergy Asthma Immunol, 2018, 120(3): 310-317. doi: 10.1016/j.anai.2017.12.011
    [14]
    YU S Q, ZHANG R X, LIU G J, et al. Microarray analysis of differentially expressed microRNAs in allergic rhinitis[J]. Am J Rhinol Allergy, 2011, 25(6): e242-e246. doi: 10.2500/ajra.2011.25.3682
    [15]
    艾红辉, 钟伟, 龙平, 等. miR-155在变应性鼻炎患者血清中的高表达[J]. 实用临床医学, 2016, 17(11): 64-65, 68, 108. https://www.cnki.com.cn/Article/CJFDTOTAL-LCSY201611026.htm
    [16]
    罗燕云, 朱殊, 阳光, 等. MiR-135a治疗小鼠变应性鼻炎的研究[J]. 华南国防医学杂志, 2015, 29(7): 489-492, 511. https://www.cnki.com.cn/Article/CJFDTOTAL-HNGY201507002.htm
    [17]
    ELBEHIDY R M, YOUSSEF D M, EL-SHAL A S, et al. MicroRNA-21 as a novel biomarker in diagnosis and response to therapy in asthmatic children[J]. Mol Immunol, 2016, 71: 107-114. doi: 10.1016/j.molimm.2015.12.015
    [18]
    LU T X, MUNITZ A, ROTHENBERG M E. MicroRNA-21 is up-regulated in allergic airway inflammation and regulates IL-12p35 expression[J]. J Immunol, 2009, 182(8): 4994-5002. doi: 10.4049/jimmunol.0803560
    [19]
    HESSAM S, SAND M, SKRYGAN M, et al. Expression of miRNA-155, miRNA-223, miRNA-31, miRNA-21, miRNA-125b, and miRNA-146a in the Inflammatory Pathway of Hidradenitis Suppurativa[J]. Inflammation, 2017, 40(2): 464-472. doi: 10.1007/s10753-016-0492-2
    [20]
    刘怡君, 曾邦兵, 曾浪. 氯雷他定联合孟鲁司特钠对变应性鼻炎患者血清ICAM-1 ECP EOS CSF的影响研究[J]. 河北医学, 2019, 25(2): 280-284. doi: 10.3969/j.issn.1006-6233.2019.02.028
    [21]
    RONDÓN C, EGUÍLUZ-GRACIA I, SHAMJI M H, et al. IgE test in secretions of patients with respiratory allergy[J]. Curr Allergy Asthma Rep, 2018, 18(12): 67. doi: 10.1007/s11882-018-0821-7
    [22]
    LU T X, LIM E J, ITSKOVICH S, et al. Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth[J]. PLoS One, 2013, 8(3): e59397. doi: 10.1371/journal.pone.0059397
    [23]
    朱振潮, 邱前辉, 陈卓, 等. 变应性鼻炎常规免疫治疗与冲击免疫治疗的临床疗效与依从性分析[J]. 临床耳鼻咽喉头颈外科杂志, 2018, 32(2): 81-86. https://www.cnki.com.cn/Article/CJFDTOTAL-LCEH201802001.htm
  • Cited by

    Periodical cited type(3)

    1. 裴双兴,叶盛开,高新,李雪. 2型糖尿病患者Hcy、Met、CysC水平与室间隔肥厚的相关性. 热带医学杂志. 2024(06): 788-792 .
    2. 赵娟,彭海林,韩俊岭,刘海飞,曹赟,邹春波. 糖尿病肾脏疾病患者血清β-羟基丁酸浓度与左心室肥厚的关系. 临床肾脏病杂志. 2023(01): 24-30 .
    3. 赵海霞. 血清Cys C浓度与2型糖尿病合并左心室肥厚患者心脏结构异常的关系. 医学理论与实践. 2023(09): 1554-1556 .

    Other cited types(3)

Catalog

    Article views (274) PDF downloads (15) Cited by(6)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return