Study on total flavonoids from Selaginella uncinata in inhibiting malignant behavior of colorectal cancer cells through circular RNA circ_0006528 pathway

SHENG Lei; LIN Hui; QIU Ni; YU Chunhua

doi:10.7619/jcmp.20213680

Journal of Clinical Medicine in Practice > 2022 > 26(4): 106-113. > DOI: 10.7619/jcmp.20213680

SHENG Lei, LIN Hui, QIU Ni, YU Chunhua. Study on total flavonoids from Selaginella uncinata in inhibiting malignant behavior of colorectal cancer cells through circular RNA circ_0006528 pathway[J]. Journal of Clinical Medicine in Practice, 2022, 26(4): 106-113. DOI: 10.7619/jcmp.20213680

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Study on total flavonoids from Selaginella uncinata in inhibiting malignant behavior of colorectal cancer cells through circular RNA circ_0006528 pathway

Department of Pharmacy, Qingdao Central Hospital in Shandong Province, Qingdao, Shandong, 266042

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Received Date: September 12, 2021
Available Online: March 21, 2022
Published Date: February 27, 2022

Graphical Abstract

Abstract

Abstract

Objective To investigate the effect of total flavonoids from Selaginella uncinata on malignant biological behavior of colorectal cancer cells and its possible mechanism.
Methods Colorectal cancer cells Caco-2 were cultured in vitro. After Caco-2 cells were treated with different concentrations (10, 20 and 40 μg/mL) of total flavonoids from Selaginella uncinata for 24 hours, or Caco-2 cells were transfected with circ_0006528 small interfering RNA, or Caco-2 cells that transfected with circ_0006528 overexpression vector were treated with 40 μg/mL total flavonoids from Selaginella uncinata for 24 hours, methyl thiazolyl tetrazolium (MTT) assay, clone formation assay, scratch assay and flow cytometry were used to detect cell proliferation viability, the number of colony formation, migration and apoptosis, respectively. The expressions of Bax and Bcl-2 proteins were detected by western blot, and the expressions of circ_0006528 and miR-330-3p were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The regulatory relationship between circ_0006528 and miR-330-3p was verified by dual luciferase reporter gene assay.
Results After Caco-2 cells were intervened with total flavonoids from Selaginella uncinata, cell viability, the number of clone formation, migration distance and the expression of Bcl-2 protein as well as circ_0006528 in Caco-2 cells were significantly decreased, but apoptosis rate and the expression of Bax protein and miR-330-3p were significantly increased (P < 0.05), and they were dose-dependent. After circ_0006528 interference, Caco-2 cell activity, the number of clone formation, migration distance and Bcl-2 protein expression were significantly decreased, while apoptosis rate and Bax protein expression were significantly increased (P < 0.05). The circ_0006528 negatively regulated miR-330-3p by targeting. Overexpression of circ_0006528 reversed the effects of total flavonoids from Selaginella uncinata on proliferation, migration and apoptosis of Caco-2 cells and expression of miR-330-3p.
Conclusion The total flavonoids from Selaginella uncinata may inhibit the proliferation and migration of colorectal cancer cells and promote cells apoptosis by regulating the circ_0006528/miR-330-3p axis.
- total flavonoids from Selaginella uncinata,
- colorectal cancer,
- circRNA circ_0006528,
- cell proliferation,
- MTT assay,
- malignant biological behavior,
- Caco-2 cells

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References (25)

References

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Study on total flavonoids from Selaginella uncinata in inhibiting malignant behavior of colorectal cancer cells through circular RNA circ_0006528 pathway