Roles of aryl hydrocarbon receptor mRNA and interleukin-22 in type 2 diabetes mellitus

LIU Yanqiu; FAN Haidi; SUN Jian; XUE Lian; MENG Yue; LIN Ning

doi:10.7619/jcmp.20214604

Journal of Clinical Medicine in Practice > 2022 > 26(13): 30-34. > DOI: 10.7619/jcmp.20214604

LIU Yanqiu, FAN Haidi, SUN Jian, XUE Lian, MENG Yue, LIN Ning. Roles of aryl hydrocarbon receptor mRNA and interleukin-22 in type 2 diabetes mellitus[J]. Journal of Clinical Medicine in Practice, 2022, 26(13): 30-34. DOI: 10.7619/jcmp.20214604

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Roles of aryl hydrocarbon receptor mRNA and interleukin-22 in type 2 diabetes mellitus

1.
Department of Clinical Laboratory, Branch Hospital of Huai'an City First People's Hospitalin Jiangsu Province, Huai'an, Jiangsu, 223002
2.
Department of Clinical Laboratory, Huai'an First Hospital Affiliated to Nanjing Medical University, Huai'an, Jiangsu, 223300

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Received Date: November 22, 2021
Available Online: July 01, 2022

Graphical Abstract

Abstract

Abstract

Objective
To investigate the expression of aryl hydrocarbon receptor (AhR) mRNA in peripheral blood mononuclear cells and serum level of interleukin-22 (IL-22) in patients with type 2 diabetes mellitus (T2DM).
Methods
A total of 49 patients with pre-diabetes mellitus, 48 patients with T2DM and 50 healthy controls were respectively included in pre-diabetes group, T2DM group and control group. The expression of AhR mRNA in peripheral blood mononuclear cells was determined by reverse transcription polymerase chain reaction (RT-PCR); serum IL-22 level was detected by enzyme-linked immunosorbent assay (ELISA) in each group. Fasting blood glucose (FBG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), cholesterol (CHO), triglyceride (TG), fasting insulin (FINS), postprandial glucose (PPG), glycosylatedhemoglobin (HbA1c) and hypersensitive C-reactive protein (hs-CRP) were measured in each group.
Results
Levels of FBG, TG, PPG, HbA1c and hs-CRP in the pre-diabetes group and the T2DM group were significantly higher than those in the control group, and HDL-C in the T2DM group was significantly lower than that in the control group (P<0.01) levels of FBG, TG, PPG and HbA1c in the T2DM group were significantly higher than those in the pre-diabetes group, while the HDL-C level was significantly lower than that in the pre-diabetes group (P<0.05 or P<0.01). FINS and serum IL-22 in the pre-diabetes group were significantly higher than those in the control group (P<0.01); the FINS, serum IL-22 and relative expression of AhR mRNA in the T2DM group were significantly higher than those in the control group and the pre-diabetes group (P<0.01). Pearson correlation analysis showed that AhR mRNA and IL-22 had no correlations with age, CHO, TG, LDL-C and body mass index (P>0.05), but were positively correlated with FBG, FINS, PPG, HbA1c and hs-CRP (P<0.05 or P<0.01).
Conclusion
Detection of AhR mRNA and IL-22 level have certain clinical significance in evaluating the disease condition and prognosis of T2DM patients, which indicate AhR mRNA and IL-22 may become new targets for the treatment of diabetes.
- type 2 diabetes mellitus,
- aryl hydrocarbon receptor,
- interleukin-22,
- fasting blood glucose,
- fasting insulin,
- glycosylated hemoglobin

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References (24)

References

[1]	WATSON J D. Type 2 diabetes as a redox disease[J]. Lancet, 2014, 383(9919): 841-843. doi: 10.1016/S0140-6736(13)62365-X
[2]	GONG F C, WU J, ZHOU P, et al. Interleukin-22 might act as a double-edged sword in type 2 diabetes and coronary artery disease[J]. Mediat Inflamm, 2016, 2016: 8254797.
[3]	满琦, 张安, 张源, 等. 脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义[J]. 山东大学学报: 医学版, 2016, 54(10): 55-59, 70. doi: 10.6040/j.issn.1671-7554.0.2015.1101
[4]	蒋茂芹, 赖铭裕, 谢芳, 等. 胃癌组织中IL-22BP、IL-22、STAT3的表达变化及意义[J]. 山东医药, 2017, 57(25): 16-19. doi: 10.3969/j.issn.1002-266X.2017.25.005
[5]	KOBAYASHI T, OKADA M, ITO S, et al. Assessment of interleukin-6 receptor inhibition therapy on periodontal condition in patients with rheumatoid arthritis and chronic periodontitis[J]. J Periodontol, 2014, 85(1): 57-67. doi: 10.1902/jop.2013.120696
[6]	YAMAJI Y, KUBOTA T, SASAGURI K, et al. Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides[J]. Infect Immun, 1995, 63(9): 3576-3581. doi: 10.1128/iai.63.9.3576-3581.1995
[7]	SUN Y, SHU R, LI C L, et al. Gram-negative periodontal bacteria induce the activation of Toll-like receptors 2 and 4, and cytokine production in human periodontal ligament cells[J]. J Periodontol, 2010, 81(10): 1488-1496. doi: 10.1902/jop.2010.100004
[8]	ANDRUKHOV O, HONG J S A, ANDRUKHOVA O, et al. Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists[J]. Sci Rep, 2017, 7(1): 12856. doi: 10.1038/s41598-017-12480-7
[9]	JEON D I, PARK S R, AHN M Y, et al. NOD1 and NOD2 stimulation triggers innate immune responses of human periodontal ligament cells[J]. Int J Mol Med, 2012, 29(4): 699-703. doi: 10.3892/ijmm.2012.878
[10]	RYBA-STANISŁAWOWSKA M, SKRZYPKOWSKA M, MYSLIWIEC M, et al. Loss of the balance between CD4(+)Foxp3(+) regulatory T cells and CD4(+)IL17A(+) Th17 cells in patients with type 1 diabetes[J]. Hum Immunol, 2013, 74(6): 701-707. doi: 10.1016/j.humimm.2013.01.024
[11]	FABBRINI E, CELLA M, MCCARTNEY S A, et al. Association between specific adipose tissue CD4+ T-cell populations and insulin resistance in obese individuals[J]. Gastroenterology, 2013, 145(2): 366-374, e1-e3. doi: 10.1053/j.gastro.2013.04.010
[12]	XU X Y, ZHENG S, YANG F, et al. Increased Th22 cells are independently associated with Th17 cells in type 1 diabetes[J]. Endocrine, 2014, 46(1): 90-98. doi: 10.1007/s12020-013-0030-z
[13]	ZHAO R X, TANG D Q, YI S N, et al. Elevated peripheral frequencies of Th22 cells: a novel potent participant in obesity and type 2 diabetes[J]. PLoS One, 2014, 9(1): e85770. doi: 10.1371/journal.pone.0085770
[14]	RYBA-STANISŁAWOWSKA M, WERNER P, BRANDT A, et al. Th9 and Th22 immune response in young patients with type 1 diabetes[J]. Immunol Res, 2016, 64(3): 730-735. doi: 10.1007/s12026-015-8765-7
[15]	唐雪娇, 肖骅, 张磊, 等. 芳香烃受体在体外高糖环境诱导心肌肥大过程中的表达[J]. 中国病理生理杂志, 2016, 32(10): 1744-1749. doi: 10.3969/j.issn.1000-4718.2016.10.003
[16]	SCHIERING C, WINCENT E, METIDJI A, et al. Feedback control of AHR signalling regulates intestinal immunity[J]. Nature, 2017, 542(7640): 242-245. doi: 10.1038/nature21080
[17]	HAMMERSCHMIDT-KAMPER C, BILJES D, MERCHES K, et al. Indole-3-carbinol, a plant nutrient and AhR-Ligand precursor, supports oral tolerance against OVA and improves peanut allergy symptoms in mice[J]. PLoS One, 2017, 12(6): e0180321. doi: 10.1371/journal.pone.0180321
[18]	BAKER N A, SHOEMAKER R, ENGLISH V, et al. Effects of adipocyte aryl hydrocarbon receptor deficiency on PCB-induced disruption of glucose homeostasis in lean and obese mice[J]. Environ Health Perspect, 2015, 123(10): 944-950. doi: 10.1289/ehp.1408594
[19]	BILJES D, HAMMERSCHMIDT-KAMPER C, KADOW S, et al. Impaired glucose and lipid metabolism in ageing aryl hydrocarbon receptor deficient mice[J]. EXCLI J, 2015, 14: 1153-1163.
[20]	SHALABY K H, LYONS-COHEN M R, WHITEHEAD G S, et al. Pathogenic T H₁₇ inflammation is sustained in the lungs by conventional dendritic cells and Toll-like receptor 4 signaling[J]. J Allergy Clin Immunol, 2018, 142(4): 1229-1242, e6. doi: 10.1016/j.jaci.2017.10.023
[21]	AFFANDI A J, SILVA-CARDOSO S C, GARCIA S, et al. CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis[J]. Eur J Immunol, 2018, 48(3): 522-531. doi: 10.1002/eji.201747195
[22]	GUO D F, CHEN Y H, WANG S J, et al. Exosomes from heat-stressed tumour cells inhibit tumour growth by converting regulatory T cells to Th17 cells via IL-6[J]. Immunology, 2018, 154(1): 132-143. doi: 10.1111/imm.12874
[23]	徐侨, 刘正霞, 鲁翔. 白介素-22在动脉粥样硬化和2型糖尿病中的作用[J]. 心血管病学进展, 2019, 40(9): 1260-1263. https://www.cnki.com.cn/Article/CJFDTOTAL-XXGB201909020.htm
[24]	HERDER C, KANNENBERG J M, CARSTENSEN-KIRBERG M, et al. Serum levels of interleukin-22, cardiometabolic risk factors and incident type 2 diabetes: KORA F4/FF4 study[J]. Cardiovasc Diabetol, 2017, 16(1): 17. doi: 10.1186/s12933-017-0498-6

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