| Citation: |
YAO Ping, CHEN Guangyu, MIAO Chunhua. Effect of Yiqi Huoxue Lishui Recipe on calcium transport and endoplasmic reticulum stress in cardiomyocytes f rats with heart failure[J]. Journal of Clinical Medicine in Practice, 2022, 26(17): 37-45. DOI: 10.7619/jcmp.20220560
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To investigate the effects of Yiqi Huoxue Lishui Recipe on calcium transport, endoplasmic reticulum stress apoptosis and the expressions of angiotensin Ⅱ (Ang Ⅱ) and transforming growth factor-β1 (TGF-β1) in cardiomyocytes of heart failure (HF) rats.
Ten healthy rats were set as Sham group, and another 45 successfully modeled rats were divided into HF group, low intervention group, medium intervention group, high intervention group and western medicine group, with 9 rats in each group. The low intervention group, middle intervention group and high intervention group were gavaged with 2.6, 5.2 and 10.4 g/(kg·d) of Yiqi Huoxue Lishui Recipe, the western medicine group was gavaged with 10 mg/(kg·d) trimetazidine, and the Sham and HF groups were gavaged with the same volume of 0.9% NaCl solution, respectively. HE staining and electron microscopy were used to observe the pathological morphology of myocardial tissue. Bradford dye binding method was used to analyze the calcium intake in the myocardial sarcoplasmic reticulum. The apoptosis of cardiomyocytes in each group was detected by Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL). Western blotting was used to detect the expressions of endoplasmic reticulum stress-related proteins[B cell lymphoma/leukemia-2 (Bcl-2), Bcl-2 associated X protein (Bax), caspase-3 protein (Caspase3)]and calcium transport-related proteins[calcium/calmodulin-dependent protein kinase Ⅱ (CAMK Ⅱ), ryanodine receptor (RyR2) and sarcoplasmic reticulum Ca2+ ATPase (SERCA2a); the positive expression rates of TGF-β1 and Ang Ⅱ in myocardial tissue were detected by immunohistochemistry.
The calcium uptake rates of rats in the Sham group, HF group, low intervention group, middle intervention group, high intervention group and western medicine group were (19.63±5.63) μmol/(min·mg), (10.25±4.11) μmol/(min·mg), (13.70±5.12) μmol/(min·mg), (14.08±4.80) μmol/(min·mg), (16.91±5.03) μmol/(min·mg), (16.65±5.10) μmol/(min·mg), respectively, there were differences among different groups (F=3.946, P=0.004). The apoptosis rates of cardiomyocytes in the Sham group, HF group, low intervention group, medium intervention group, high intervention group and western medicine group were (7.52±2.01)%, (30.63±6.58)%, (19.14±5.03)%, (18.41±5.21)%, (13.61±3.65)% and (13.17±4.11)%, respectively, there were differences among different groups (F=28.900, P < 0.001); compared with the Sham group, Bcl-2 and SERCA2a levels in the HF group in myocardial tissue of rats were decreased, and Bax, Caspase3, CaMK Ⅱ, RyR2, TGF-β1 and Ang Ⅱ expression were increased (P < 0.05). Compared with the HF group, expressions of Bcl-2 and SERCA2a were increased in the low, medium and high intervention groups and western medicine group, Bax, Caspase3, CaMK Ⅱ, RyR2, TGF-β1 and Ang Ⅱ were all decreased (P < 0.05). There were no significant differences in the low intervention group in above eight indicators compared with the medium intervention group (P>0.05). Compared with the medium intervention group, the high intervention group showed significant differences in above indexes (P < 0.05), but there was no significant differences compared with the western medicine group in above indexes (P>0.05).
Yiqi Huoxue Lishui Formula can improve calcium transport in heart failure rats, and reduce myocardial apoptosis by inhibiting endoplasmic reticulum stress in a concentration-dependent manner. The mechanism may be related to the inhibition of TGF-β1 and Ang Ⅱ expression.
| [1] |
MONZO L, GAUDIO C, CICOGNA F, et al. Impact of sacubitril/valsartan on implantable defibrillator eligibility in heart failure: a real-world experience[J]. Eur Rev Med Pharmacol Sci, 2021, 25(18): 5690-5700.
|
| [2] |
KONIARI I, ARTOPOULOU E, VELISSARIS D, et al. Biomarkers in the clinical management of patients with atrial fibrillation and heart failure[J]. J Geriatr Cardiol, 2021, 18(11): 908-951.
|
| [3] |
LEE Y, CHAKRABORTY S, MUTHUCHAMY M. Roles of sarcoplasmic reticulum Ca2+ ATPase pump in the impairments of lymphatic contractile activity in a metabolic syndrome rat model[J]. Sci Rep, 2020, 10(1): 12320. doi: 10.1038/s41598-020-69196-4
|
| [4] |
HUANG A N, LI H H, ZENG C, et al. Endogenous CCN5 participates in angiotensin Ⅱ/TGF-β1 networking of cardiac fibrosis in high angiotensin Ⅱ-induced hypertensive heart failure[J]. Front Pharmacol, 2020, 11: 1235. doi: 10.3389/fphar.2020.01235
|
| [5] |
贺小芳, 唐蓓蕾, 王显, 等. 益气活血利水方治疗冠心病心力衰竭气虚血瘀水饮证患者疗效分析[J]. 现代中医临床, 2020, 27(3): 9-12. doi: 10.3969/j.issn.2095-6606.2020.03.003
|
| [6] |
刘俊杰, 赵慧辉, 张建, 等. 基于"虚、毒、瘀"浅述芪参颗粒治疗慢性心力衰竭气虚血瘀证中医理论依据[J]. 世界中医药, 2019, 14(6): 1447-1449. doi: 10.3969/j.issn.1673-7202.2019.06.019
|
| [7] |
徐香玲, 杨继, 赵英强. 附子温阳功效在治疗心力衰竭病中的临床应用进展[J]. 现代中西医结合杂志, 2021, 30(31): 3520-3525. doi: 10.3969/j.issn.1008-8849.2021.31.022
|
| [8] |
LI A, STROIK D, SCHAAF T, et al. Biophysics of the SERCA2A/DWORF complex, implications for treatment of heart failure[J]. Biophys J, 2020, 118(3): 593a.
|
| [9] |
ONAL B, GRATZ D, HUND T J. Ca2+/calmodulin-dependent kinase Ⅱ-dependent regulation of atrial myocyte late Na+ current, Ca2+ cycling, and excitability: a mathematical modeling study[J]. Am J Physiol Heart Circ Physiol, 2017, 313(6): H1227-H1239. doi: 10.1152/ajpheart.00185.2017
|
| [10] |
王柳, 郑颖, 林德洪, 等. 美托洛尔片联合曲美他嗪片治疗老年冠心病心力衰竭患者的临床研究[J]. 中国临床药理学杂志, 2020, 36(15): 2175-2177. https://www.cnki.com.cn/Article/CJFDTOTAL-GLYZ202015004.htm
|
| [11] |
彭朗, 鲁晓斌, 梅应兵, 等. 益气活血利水方联合螺内酯治疗老年射血分数保留心力衰竭的效果[J]. 中国医药导报, 2020, 17(17): 57-61. https://www.cnki.com.cn/Article/CJFDTOTAL-YYCY202017015.htm
|
| [12] |
于春泉, 王怡, 李芳, 等. 益气活血方对慢性心力衰竭大鼠心肌NCX与SERCA2a mRNA及蛋白表达水平的影响[J]. 中草药, 2011, 42(3): 555-558. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201103036.htm
|
| [13] |
吴佳妮. 基于网络药理学技术探讨益气活血方治疗心肌缺血的分子机制研究[D]. 北京: 北京中医药大学, 2016.
|
| [14] |
XU L Y, GAO X, YANG P, et al. EHMT2 inhibitor BIX-01294 induces endoplasmic Reticulum stress mediated apoptosis and autophagy in diffuse large B-cell lymphoma cells[J]. J Cancer, 2021, 12(4): 1011-1022. doi: 10.7150/jca.48310
|
| [15] |
CHIKKA M R, MCCABE D D, TYRA H M, et al. C/EBP homologous protein (CHOP) contributes to suppression of metabolic genes during endoplasmic Reticulum stress in the liver[J]. J Biol Chem, 2013, 288(6): 4405-4415. doi: 10.1074/jbc.M112.432344
|
| [16] |
刘璐, 李思耐, 高群, 等. 党参对压力负荷致心衰小鼠电生理重构的影响[J]. 中西医结合心脑血管病杂志, 2018, 16(3): 287-290. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYY201803009.htm
|
| [17] |
张颖, 肖骏, 徐艺, 等. 羟基红花黄色素A对缺氧/复氧心肌细胞增殖与凋亡的影响[J]. 中国临床药理学杂志, 2020, 36(24): 3995-3998. https://www.cnki.com.cn/Article/CJFDTOTAL-GLYZ202024008.htm
|
| [18] |
徐京育, 李宗瑛, 叶婷, 等. 强心胶囊对心力衰竭模型大鼠AngⅡAT1R的影响[J]. 中国急救医学, 2020, 40(4): 348-351. doi: 10.3969/j.issn.1002-1949.2020.04.017
|
| [19] |
SUI X Z, WEI H C, WANG D C. Novel mechanism of cardiac protection by valsartan: synergetic roles of TGF-β1 and HIF-1α in Ang Ⅱ-mediated fibrosis after myocardial infarction[J]. J Cell Mol Med, 2015, 19(8): 1773-1782. doi: 10.1111/jcmm.12551
|
| [20] |
狄宁宁, 崔振川, 孙云静, 等. 丹参酮ⅡA磺酸钠联合卡维地洛对心力衰竭患者心功能及内分泌激素水平的影响[J]. 中国药业, 2021, 30(5): 51-54. doi: 10.3969/j.issn.1006-4931.2021.05.014
|
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