GUI Ping, ZENG Xiancong. Epithelial expression of stromal interaction molecule 1/calcium release-activated calcium channel protein 1 in asthmatic airway hyperresponsiveness and its role[J]. Journal of Clinical Medicine in Practice, 2023, 27(1): 53-59. DOI: 10.7619/jcmp.20221504
Citation: GUI Ping, ZENG Xiancong. Epithelial expression of stromal interaction molecule 1/calcium release-activated calcium channel protein 1 in asthmatic airway hyperresponsiveness and its role[J]. Journal of Clinical Medicine in Practice, 2023, 27(1): 53-59. DOI: 10.7619/jcmp.20221504

Epithelial expression of stromal interaction molecule 1/calcium release-activated calcium channel protein 1 in asthmatic airway hyperresponsiveness and its role

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  • Received Date: May 10, 2022
  • Available Online: February 01, 2023
  • Objective 

    To investigate the epithelial expression of stromal interaction molecule 1 (STIM1)/calcium release-activated calcium channel protein 1(Orail)in airway hyperresponsiveness (AHR) and its role.

    Methods 

    The mice were divided into control group, ovalbumin (OVA) group and OVA+sh-STIM1 group, with 12 mice in each group. Except for the control group, the other groups established OVA-induced acute asthma mice models. The OVA+sh-STIM1 group was given sh-STIM1 intranasally for intervention. The expression of STIM1 in lung tissue was checked by immunofluorescence staining. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of inflammatory factors in mouse bronchoalveolar lavage fluid (BALF). The effects of STIM1 knockdown on contractility and Ca2+ influx of airway smooth muscle (ASM) cells in human airway smooth muscle cells (HASMC) were analyzed.

    Results 

    STIM1 was mainly expressed in bronchial epithelium, and intranasal administration of sh-STIM1 could relieve AHR and inflammation in mice. In vitro, STIM1 knockdown inhibited the contraction of HASMC cells by blocking Oria1 mediated calcium influx ex vivo.

    Conclusion 

    STIM1 in epithelial cells regulates airway smooth muscle contraction and AHR, and its mechanism of action is related to the activation of Oria1-mediated calcium influx.

  • [1]
    DRISCOLL A J, ARSHAD S H, BONT L, et al. Does respiratory syncytial virus lower respiratory illness in early life cause recurrent wheeze of early childhood and asthma Critical review of the evidence and guidance for future studies from a World Health Organization-sponsored meeting[J]. Vaccine, 2020, 38(11): 2435-2448. doi: 10.1016/j.vaccine.2020.01.020
    [2]
    HUANG W C, LIU C Y, SHEN S C, et al. Protective effects of licochalcone A improve airway hyper-responsiveness and oxidative stress in a mouse model of asthma[J]. Cells, 2019, 8(6): 617. doi: 10.3390/cells8060617
    [3]
    KOGAN V, MILLSTEIN J, LONDON S J, et al. Genetic-epigenetic interactions in asthma revealed by a genome-wide gene-centric search[J]. Hum Hered, 2018, 83(3): 130-152. doi: 10.1159/000489765
    [4]
    JHA A, CHUNG W Y, VACHEL L, et al. Anoctamin 8 tethers endoplasmic Reticulum and plasma membrane for assembly of Ca2+ signaling complexes at the ER/PM compartment[J]. EMBO J, 2019, 38(12): e101452.
    [5]
    SUNDARAM A, CHEN C, ISIK REED N, et al. Dual antagonists of α5β1/αvβ1 integrin for airway hyperresponsiveness[J]. Bioorg Med Chem Lett, 2020, 30(22): 127578. doi: 10.1016/j.bmcl.2020.127578
    [6]
    ZHOU Y B, GU P, LI J, et al. Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation[J]. Oncol Rep, 2017, 38(5): 2629-2636. doi: 10.3892/or.2017.5961
    [7]
    刘芳. 西替利嗪滴剂与布地奈德吸入剂联用对哮喘急性发作患儿肺功能指标的影响[J]. 实用临床医药杂志, 2020, 24(3): 87-89, 93. doi: 10.7619/jcmp.202003025
    [8]
    DU X Y, ZHI J, YANG D, et al. Research progress in the mechanism of calcium ion on contraction and relaxation of airway smooth muscle cells[J]. J Recept Signal Transduct Res, 2021, 41(2): 117-122. doi: 10.1080/10799893.2020.1806315
    [9]
    MICHELUCCI A, GARCÍA-CASTAÑEDA M, BONCOMPAGNI S, et al. Role of STIM1/ORAI1-mediated store-operated Ca2+ entry in skeletal muscle physiology and disease[J]. Cell Calcium, 2018, 76: 101-115. doi: 10.1016/j.ceca.2018.10.004
    [10]
    CONTE E, IMBRICI P, MANTUANO P, et al. Alteration of STIM1/Orai1-mediated SOCE in skeletal muscle: impact in genetic muscle diseases and beyond[J]. Cells, 2021, 10(10): 2722. doi: 10.3390/cells10102722
    [11]
    ZHANG X Y, ZHAO Z X, MA L J, et al. The effects of transient receptor potential channel (TRPC) on airway smooth muscle cell isolated from asthma model mice[J]. J Cell Biochem, 2018, 119(7): 6033-6044. doi: 10.1002/jcb.26801
    [12]
    MARONE G, GRANATA F, PUCINO V, et al. The intriguing role of interleukin 13 in the pathophysiology of asthma[J]. Front Pharmacol, 2019, 10: 1387. doi: 10.3389/fphar.2019.01387
    [13]
    PARULEKAR A D, KAO C C, DIAMANT Z, et al. Targeting the interleukin-4 and interleukin-13 pathways in severe asthma: current knowledge and future needs[J]. Curr Opin Pulm Med, 2018, 24(1): 50-55. doi: 10.1097/MCP.0000000000000436
    [14]
    HUANG Z Q, LIU J, ONG H H, et al. Interleukin-13 alters tight junction proteins expression thereby compromising barrier function and dampens rhinovirus induced immune responses in nasal epithelium[J]. Front Cell Dev Biol, 2020, 8: 572749. doi: 10.3389/fcell.2020.572749
    [15]
    FAN H T, HUANG H J, HU L, et al. The activation of STIM1 mediates S-phase arrest and cell death in paraquat induced acute lung intoxication[J]. Toxicol Lett, 2018, 292: 123-135. doi: 10.1016/j.toxlet.2018.04.030
    [16]
    MALI V, HADDOX S, BELMADANI S, et al. Essential role for smooth muscle cell stromal interaction molecule-1 in myocardial infarction[J]. J Hypertens, 2018, 36(2): 377-386. doi: 10.1097/HJH.0000000000001518
    [17]
    JANULAITYTE I, JANUSKEVICIUS A, KALINAUSKAITE-ZUKAUSKE V, et al. Asthmatic eosinophils promote contractility and migration of airway smooth muscle cells and pulmonary fibroblasts in vitro[J]. Cells, 2021, 10(6): 1389. doi: 10.3390/cells10061389
    [18]
    WANG Y X, WANG L, ZHENG Y M. Canonical transient potential receptor-3 channels in normal and diseased airway smooth muscle cells[J]. Adv Exp Med Biol, 2020, 1131: 471-487.

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