FU Chenyang, ZHOU Ping, ZHANG Pin, HUI Li. Correlations of vaginal microecology with human papilloma virus and cervical lesion[J]. Journal of Clinical Medicine in Practice, 2023, 27(16): 37-42. DOI: 10.7619/jcmp.20221628
Citation: FU Chenyang, ZHOU Ping, ZHANG Pin, HUI Li. Correlations of vaginal microecology with human papilloma virus and cervical lesion[J]. Journal of Clinical Medicine in Practice, 2023, 27(16): 37-42. DOI: 10.7619/jcmp.20221628

Correlations of vaginal microecology with human papilloma virus and cervical lesion

More Information
  • Received Date: May 22, 2022
  • Revised Date: December 01, 2022
  • Available Online: September 05, 2023
  • Objective 

    To evaluate the associations of vaginal microecology with human papilloma virus (HPV) infection and cervical lesions.

    Methods 

    A total of 772 patients with abnormal preliminary screening results of thin layer liquid based cytology (TCT) were selected, were graded according to the degree of lesions, and the infection of high-risk human papillomavirus (HR-HPV) was analyzed. A total of 156 patients without intraepithelial pathological cells and malignant cells (NILM) and negative HPV typing were selected in the initial TCT screening during the same period. All patients were divided into TCT abnormal HPV-positive patients, TCT abnormal HPV-negative patients and TCT normal HPV-negative patients. All patients underwent vaginal microecological examination. Associations of vaginal microecology with HR-HPV and cervical lesion were evaluated.

    Results 

    Among 772 patients with abnormal TCT results, the HPV infection rate was 48.06%. Among them, multiple types of infection accounted for 22.37% of HPV infections. The higher infection rate of the subtypes were HPV52, HPV16 and HPV58. The HPV infection rate in the 772 patients with high-grade squamous intraepithelial lesions (HSIL) was higher than that in the patients with low-grade squamous intraepithelial lesions (LSIL), atypical squamous cells of undetermined significance (ASCUS) and atypical glandular epithelial cells (AGC), and the difference was statistically significant (P < 0.05). In the vaginal microecological study, the positive rates of sialic ananase, leukocyte esterase and bacterial vaginosis (BV) in TCT abnormal HPV-positive patients were compared with those in TCT abnormal HPV-negative patients, and the differences were statistically significant (P < 0.05). The level of hydrogen peroxide, sialic anhydrolase, leucocytoesterase, β-glucuronidase, coagulase positive rate, BV, aerobic vaginitis (AV), vaginal mixed infection, proportion of vaginal microecological disorders, cleanliness (Ⅲ to Ⅳ) and lactobacillus positive rate in HPV-positive patients with abnormal TCT were compared with those in normal HPV-negative patients, the difference was statistically significant (P < 0.05). Hydrogen peroxide, sialic anhydrase, leocytoesterase positive, BV, co-infection, vaginal dysbiosis, cleanliness (Ⅲ to Ⅳ) were risk factors for HR-HPV positive and/or cervical cytology abnormalities (OR>1, P < 0.05), and lactobacillus positive was a protective factor for HR-HPV positive and/or cervical cytological abnormalities (OR < 1, P < 0.05).

    Conclusion 

    Vaginal microecology is related to HR-HPV infection and cervical lesions, and the imbalance of vaginal microecology is more likely to lead to HR-HPV infection and promote the occurrence and development of cervical lesions.

  • [1]
    VRIEND H J, BOGAARDS J A, VAN BERGEN J E, et al. Incidence and persistence of carcinogenic genital human papillomavirus infections in young women with or without Chlamydia trachomatis co-infection[J]. Cancer Med, 2015, 4(10): 1589-1598. doi: 10.1002/cam4.496
    [2]
    YOUNG V B. The role of the microbiome in human health and disease: an introduction for clinicians[J]. BMJ, 2017, 356: j831.
    [3]
    MENDLING W. Vaginal microbiota[J]. Adv Exp Med Biol, 2016, 902: 83-93.
    [4]
    AUDIRAC-CHALIFOUR A, TORRES-POVEDA K, BAHENA-ROMÁN M, et al. Cervical microbiome and cytokine profile at various stages of cervical cancer: a pilot study[J]. PLoS One, 2016, 11(4): e0153274. doi: 10.1371/journal.pone.0153274
    [5]
    DEPUYDT C E, JONCKHEERE J, BERTH M, et al. Serial type-specific human papillomavirus (HPV) load measurement allows differentiation between regressing cervical lesions and serial virion productive transient infections[J]. Cancer Med, 2015, 4(8): 1294-1302. doi: 10.1002/cam4.473
    [6]
    HOPPE-SEYLER K, BOSSLER F, BRAUN J A, et al. The HPV E6/E7 oncogenes: key factors for viral carcinogenesis and therapeutic targets[J]. Trends Microbiol, 2018, 26(2): 158-168. doi: 10.1016/j.tim.2017.07.007
    [7]
    LI S Y, HONG X L, WEI Z T, et al. Ubiquitination of the HPV oncoprotein E6 is critical for E6/E6AP-mediated p53 degradation[J]. Front Microbiol, 2019, 10: 2483. doi: 10.3389/fmicb.2019.02483
    [8]
    LI Y W, LIU X L, HAN C X, et al. Prevalence and genotype distribution of high-risk human papillomavirus in 34 420 cases in Yangzhou city, Jiangsu Province, China[J]. J Med Virol, 2021, 93(8): 5095-5102. doi: 10.1002/jmv.27012
    [9]
    ZHU X Y, WANG Y X, LV Z, et al. Prevalence and genotype distribution of high-risk HPV infection among women in Beijing, China[J]. J Med Virol, 2021, 93(8): 5103-5109. doi: 10.1002/jmv.27013
    [10]
    范洁琳. HPV感染与宫颈病变关系及相关危险因素研究[D]. 长沙: 中南大学, 2014.
    [11]
    王秀芳, 赵辉. 人乳头瘤病毒感染和阴道微生态状况对宫颈鳞状上皮内病变的影响[J]. 中华医院感染学杂志, 2021, 31(2): 268-271. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY202102025.htm
    [12]
    LIANG Y J, CHEN M J, QIN L, et al. A meta-analysis of the relationship between vaginal microecology, human papillomavirus infection and cervical intraepithelial neoplasia[J]. Infect Agent Cancer, 2019, 14: 29. doi: 10.1186/s13027-019-0243-8
    [13]
    SNIJDERS P J, VAN DEN BRULE A J, MEIJER C J. The clinical relevance of human papillomavirus testing: relationship between analytical and clinical sensitivity[J]. J Pathol, 2003, 201(1): 1-6. doi: 10.1002/path.1433
    [14]
    黄斐, 赵文龙, 楼颖素, 等. 高危型人乳头瘤病毒感染与阴道微生态及宫颈局部细胞免疫的相关性[J]. 中国微生态学杂志, 2022, 34(4): 463-466. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGWS202204017.htm
    [15]
    MACKLAIM J M, FERNANDES A D, DI BELLA J M, et al. Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis[J]. Microbiome, 2013, 1(1): 12. doi: 10.1186/2049-2618-1-12
    [16]
    MITRA A, MACINTYRE D A, MARCHESI J R, et al. The vaginal microbiota, human papillomavirus infection and cervical intraepithelial neoplasia: what do we know and where are we going next[J]. Microbiome, 2016, 4(1): 58. doi: 10.1186/s40168-016-0203-0
    [17]
    陈荣, 陈花, 汤莉. 阴道分泌物功能酶检测联合常规镜检在阴道微生态改变中的应用分析[J]. 实用妇科内分泌电子杂志, 2020, 7(22): 130-130, 140. https://www.cnki.com.cn/Article/CJFDTOTAL-FKDZ202022090.htm
    [18]
    KOVACHEV S M. Cervical cancer and vaginal microbiota changes[J]. Arch Microbiol, 2020, 202(2): 323-327. doi: 10.1007/s00203-019-01747-4
    [19]
    DOLS J A, REID G, KORT R, et al. PCR-based identification of eight Lactobacillus species and 18 hr-HPV genotypes in fixed cervical samples of South African women at risk of HIV and BV[J]. Diagn Cytopathol, 2012, 40(6): 472-477. doi: 10.1002/dc.21786
    [20]
    O'HANLON D E, MOENCH T R, CONE R A. In vaginal fluid, bacteria associated with bacterial vaginosis can be suppressed with lactic acid but not hydrogen peroxide[J]. BMC Infect Dis, 2011, 11: 200. doi: 10.1186/1471-2334-11-200
    [21]
    HARWICH M D Jr, SERRANO M G, FETTWEIS J M, et al. Genomic sequence analysis and characterization of Sneathia amnii sp. nov[J]. BMC Genomics, 2012, 13(Suppl 8): S4.
    [22]
    NAWROT R, KAMIENIARZ K, MALINOWSKA M, et al. The prevalence of leptotrichia amnionii in cervical swabs of HPV positive and negative women[J]. Eur J Gynaecol Oncol, 2010, 31(4): 425-428.
    [23]
    张鹏玲. 宫颈上皮内瘤变和宫颈癌患者阴道微环境因子与阴道菌群的相关性研究[J]. 医学理论与实践, 2021, 34(20): 3617-3619. https://www.cnki.com.cn/Article/CJFDTOTAL-YXLL202120057.htm
    [24]
    CHEN Y L, QIU X D, WANG W J, et al. Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort[J]. BMC Infect Dis, 2020, 20(1): 629.
    [25]
    ŁANIEWSKI P, CUI H Y, ROE D J, et al. Features of the cervicovaginal microenvironment drive cancer biomarker signatures in patients across cervical carcinogenesis[J]. Sci Rep, 2019, 9(1): 7333.
  • Related Articles

    [1]HUA Zhenyue, YANG Qiuyue, MEI Xue, LUO Kunlun, LIU Wenfeng, TAO Weili, XU Tingting. Correlation between postoperative mindfulness level and family care in patients with liver cancer[J]. Journal of Clinical Medicine in Practice, 2021, 25(19): 115-118. DOI: 10.7619/jcmp.20212011
    [2]TANG Xuefeng, QIAN Yanjun, CHEN Min. Correlation between formation of carotid atheroscleroticplaque and Helicobacter pylori infection in people with health examination[J]. Journal of Clinical Medicine in Practice, 2021, 25(5): 33-36. DOI: 10.7619/jcmp.20200928
    [3]ZHOU Zhuolin, BI Chunchao, SHAO Juan, XU Zhiguo, LIU Jing. Analysis on correlations between blood lipid, blood glucose metabolic levels as well as blood pressure and diabetic retinopathy[J]. Journal of Clinical Medicine in Practice, 2020, 24(10): 49-52. DOI: 10.7619/jcmp.202010012
    [4]YU Xiuxia, CAO Xiaoming. Correlation between Helicobacter pylori infection and immune thrombocytopenia[J]. Journal of Clinical Medicine in Practice, 2020, 24(2): 108-110. DOI: 10.7619/jcmp.202002031
    [5]ZHENG Jianjun. Value on detection of high-risk human papilloma virus, squamous cell carcinoma antigen and squamous cell associated carcinoma antigen after radical resection of cervical cancer[J]. Journal of Clinical Medicine in Practice, 2019, 23(23): 58-60. DOI: 10.7619/jcmp.201923017
    [6]LIU Hongfu, ZHANG Lei, YANG Shuhua, ZHANG Xinhua, XIE Rongzhen, DENG Longying, DENG Wei. Correlation between HLA-G gene polymorphism and gastric cancer in Han nationality[J]. Journal of Clinical Medicine in Practice, 2017, (19): 70-72. DOI: 10.7619/jcmp.201719020
    [7]CAO Ruihong, JIANG Jianyu, LI Xianliang. Correlation between hypertension and serum lipoprotein (a) level[J]. Journal of Clinical Medicine in Practice, 2017, (17): 48-50. DOI: 10.7619/jcmp.201717014
    [8]XIAO Zhanghong, DING Lidong, CAO Xiaoping, LIU Cailing, WU Sisi, KE Kaifu. Correlation between carotid atherosclerotic plaque and microemboli in patients with acute cerebral infarction[J]. Journal of Clinical Medicine in Practice, 2016, (21): 14-16. DOI: 10.7619/jcmp.201621005
    [9]YANG Xiaoqin. Correlation between expressions of proliferating cell nuclear antigen, Ki-67 and HPV infection in cervical lesions[J]. Journal of Clinical Medicine in Practice, 2015, (17): 57-60. DOI: 10.7619/jcmp.201517018
    [10]LI Fei. Correlation between histidine triad, inhibitory factor in lung cancer 1 gene expressions and occurrences of cervical cancer and human papillomavirus infection[J]. Journal of Clinical Medicine in Practice, 2015, (7): 97-100. DOI: 10.7619/jcmp.201507028

Catalog

    Article views (177) PDF downloads (32) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return