Methylation status of Septin 9 in peripheral blood and its relationship with colorectal cancer

LI Tuojian; ZHANG Chao; CHEN Zongtao; LI Xinliang

doi:10.7619/jcmp.20222618

Journal of Clinical Medicine in Practice > 2022 > 26(23): 5-8, 13. > DOI: 10.7619/jcmp.20222618

LI Tuojian, ZHANG Chao, CHEN Zongtao, LI Xinliang. Methylation status of Septin 9 in peripheral blood and its relationship with colorectal cancer[J]. Journal of Clinical Medicine in Practice, 2022, 26(23): 5-8, 13. DOI: 10.7619/jcmp.20222618

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Methylation status of Septin 9 in peripheral blood and its relationship with colorectal cancer

1.
Health Management Center, the First Affiliated Hospital of Army Medical University of the Chinese People's Liberation Army, Chongqing, 400038
2.
Department of Oncology, Shenzhen Hospital of Peking University, Shenzhen, Guangdong, 518036

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Received Date: August 22, 2022
Available Online: December 01, 2022

Graphical Abstract

Abstract

Abstract

Objective
To detect the methylation status of Septin 9 in the peripheral blood of patients with colorectal cancer (CRC) and analyze the relationship between the methylation of Septin 9 and CRC.
Methods
A total of 120 patients with CRC were selected as research objects, and the relationship between the methylation status of Septin 9 in the peripheral blood and the medical materials was analyzed; the serum exosomes isolated from patients with CRC and healthy people were used to treat the CRC cells, and the proliferation and apoptosis of CRC cells were detected.
Results
A total of 1 586 people with health examinations were screened for Septin 9, and 56 of whom were positive for methylation of Septin 9, with a positive rate of 3.5%. Among patients with Septin 9 positive, there were 20 cases (35.7%) with abnormal gastrointestinal status (gastrointestinal inflammation or polyps), while only 5.4% (83/1 530) of patients with Septin 9 negative had abnormal gastrointestinal status, which indicated that the methylation status of Septin 9 in the peripheral blood was closely related to the gastrointestinal status. Among 120 CRC patients, the positive rate of methylation of Septin 9 was 78.4% (73/93); in the comparison of different differentiation degrees, the positive rates of Septin 9 methylation in patients with highly differentiated, moderately differentiated and poorly differentiated CRC were 52.6% (10/19), 79.7% (59/74) and 88.9% (24/27) respectively, and the positive rate of Septin 9 methylation in peripheral blood of patients with poorly differentiated CRC was significantly higher than that of patients with highly differentiated CRC (P=0.006). The sensitivity and specificity of Septin 9 methylation in the peripheral blood were 72.3% and 92.5% respectively. Western blot results showed that the expression of Septin 9 in the exosomes of CRC patients was significantly lower than that in the normal people, while the methylation level of Septin 9 in the exosomes was significantly higher than that in the healthy people (P=0.009, 0.003). The results of this study showed that serum exosomes of CRC patients could significantly promote cell proliferation and inhibit cell apoptosis (P < 0.05 or P < 0.01).
Conclusion
Septin 9 can be used as a marker for early screening of CRC, and the mechanism of Septin 9 may be the proliferation and apoptosis resistance of CRC induced by serum exosomes in patients with CRC.
- colorectal cancer,
- Septin 9,
- exosomes,
- carcinoembryonic antigen,
- carbohydrate antigen 199,
- diagnosis

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References (16)

References

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Methylation status of Septin 9 in peripheral blood and its relationship with colorectal cancer