Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma

HAO Runmei; LIU Jing; LIU Jie; ZHANG Ting

doi:10.7619/jcmp.20223262

Journal of Clinical Medicine in Practice > 2023 > 27(5): 72-75, 81. > DOI: 10.7619/jcmp.20223262

HAO Runmei, LIU Jing, LIU Jie, ZHANG Ting. Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 72-75, 81. DOI: 10.7619/jcmp.20223262

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Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma

Department of Otolaryngology, Head and Neck Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061

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Received Date: November 01, 2022
Revised Date: December 06, 2022
Available Online: April 06, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore the expression and diagnostic value of long non-coding RNA (LncRNA) X-inactive specific transcript (XIST) and microRNA-146-5p (miR-146-5p) in thyroid cancer tissue.
Methods
A total of 92 patients with thyroid cancer were selected as research objects. All patients underwent surgical resection, and thyroid cancer tissues and adjacent normal tissues were collected during the operation. The relationships between the levels of LncRNA XIST, miR-146-5p and the clinical pathological characteristics of patients were analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of LncRNA XIST and miR-146-5p alone and their combination in thyroid cancer.
Results
The expression level of LncRNA XIST in the thyroid cancer tissue was (0.65±0.17), which was significantly lower than (1.02±0.18) in the adjacent tissues, and the expression level of miR-146-5p was (2.56±0.87), which was significantly higher than (1.07±0.25) in the adjacent tissues (P < 0.05). The expression levels of LncRNA XIST in thyroid cancer patients with clinical stage Ⅲ to Ⅳ and cervical lymph node metastasis were significantly lower than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis, and the expression level of miR-146-5p was significantly higher than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis (P < 0.05). ROC curve showed that the area under curve (AUC) of LncRNA XIST combined with miR-146-5p in was significantly higher than that of LncRNA XIST and miR-146-5p alone (P < 0.05).
Conclusion
The expression of LncRNA XIST is down-regulated and miR-146-5p is up-regulated in thyroid cancer. Both of them are related to clinical stage and cervical lymph node metastasis, and have certain diagnostic value for thyroid cancer.
- thyroid cancer,
- long non-coding RNA,
- X-inactive specific transcript,
- microRNA-146-5p

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References (15)

References

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Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma