HAO Runmei, LIU Jing, LIU Jie, ZHANG Ting. Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 72-75, 81. DOI: 10.7619/jcmp.20223262
Citation: HAO Runmei, LIU Jing, LIU Jie, ZHANG Ting. Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 72-75, 81. DOI: 10.7619/jcmp.20223262

Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma

More Information
  • Received Date: November 01, 2022
  • Revised Date: December 06, 2022
  • Available Online: April 06, 2023
  • Objective 

    To explore the expression and diagnostic value of long non-coding RNA (LncRNA) X-inactive specific transcript (XIST) and microRNA-146-5p (miR-146-5p) in thyroid cancer tissue.

    Methods 

    A total of 92 patients with thyroid cancer were selected as research objects. All patients underwent surgical resection, and thyroid cancer tissues and adjacent normal tissues were collected during the operation. The relationships between the levels of LncRNA XIST, miR-146-5p and the clinical pathological characteristics of patients were analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of LncRNA XIST and miR-146-5p alone and their combination in thyroid cancer.

    Results 

    The expression level of LncRNA XIST in the thyroid cancer tissue was (0.65±0.17), which was significantly lower than (1.02±0.18) in the adjacent tissues, and the expression level of miR-146-5p was (2.56±0.87), which was significantly higher than (1.07±0.25) in the adjacent tissues (P < 0.05). The expression levels of LncRNA XIST in thyroid cancer patients with clinical stage Ⅲ to Ⅳ and cervical lymph node metastasis were significantly lower than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis, and the expression level of miR-146-5p was significantly higher than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis (P < 0.05). ROC curve showed that the area under curve (AUC) of LncRNA XIST combined with miR-146-5p in was significantly higher than that of LncRNA XIST and miR-146-5p alone (P < 0.05).

    Conclusion 

    The expression of LncRNA XIST is down-regulated and miR-146-5p is up-regulated in thyroid cancer. Both of them are related to clinical stage and cervical lymph node metastasis, and have certain diagnostic value for thyroid cancer.

  • [1]
    杨元芳, 赵焕, 郝莉. 分化型甲状腺癌患者术后复发转移的临床指标及相关影响因素分析[J]. 实用癌症杂志, 2022, 37(10): 1619-1621, 1631. https://www.cnki.com.cn/Article/CJFDTOTAL-SYAZ202210013.htm
    [2]
    JAVED Z, AHMED SHAH F, RAJABI S, et al. LncRNAs as potential therapeutic targets in thyroid cancer[J]. Asian Pac J Cancer Prev, 2020, 21(2): 281-287. doi: 10.31557/APJCP.2020.21.2.281
    [3]
    许俊, 罗云春, 杨振华. 长链非编码RNA X染色体失活特异转录物调控微小RNA-335对胃癌的增殖和迁移的影响[J]. 中华实验外科杂志, 2021, 38(3): 413-416. https://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZGYL201404001230.htm
    [4]
    DU Y L, LIANG Y, CAO Y, et al. LncRNA XIST promotes migration and invasion of papillary thyroid cancer cell by modulating miR-101-3p/CLDN1 axis[J]. Biochem Genet, 2021, 59(2): 437-452. doi: 10.1007/s10528-020-09985-8
    [5]
    SUN X H, CUI S C, FU X F, et al. MicroRNA-146-5p promotes proliferation, migration and invasion in lung cancer cells by targeting claudin-12[J]. Cancer Biomark, 2019, 25(1): 89-99. doi: 10.3233/CBM-182374
    [6]
    林岩松, 李娇. 2015年美国甲状腺学会《成人甲状腺结节与分化型甲状腺癌诊治指南》解读: 分化型甲状腺癌131I治疗新进展[J]. 中国癌症杂志, 2016, 26(1): 1-12. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGAZ201601002.htm
    [7]
    殷德涛, 唐艺峰. 甲状腺滤泡癌的诊断和治疗热点[J]. 西安交通大学学报: 医学版, 2019, 40(3): 339-342, 351. https://www.cnki.com.cn/Article/CJFDTOTAL-XAYX201903001.htm
    [8]
    YAO F Z, HE R, JIANG Y C, et al. LncRNA XIST promotes migration of Wilms' tumor cells through modulation of microRNA-193a-5p[J]. Eur Rev Med Pharmacol Sci, 2020, 24(19): 9889-9898.
    [9]
    LIU J L, YAO L, ZHANG M Y, et al. Downregulation of LncRNA-XIST inhibited development of non-small cell lung cancer by activating miR-335/SOD2/ROS signal pathway mediated pyroptotic cell death[J]. Aging (Albany NY), 2019, 11(18): 7830-7846.
    [10]
    陈超, 张伟丽, 冯长松. lncRNA PCAT19靶向miR-143-3p通过信号通路PI3K/Akt对甲状腺癌细胞增殖和凋亡的影响及机制[J]. 中国老年学杂志, 2020, 40(8): 1712-1717. https://www.cnki.com.cn/Article/CJFDTOTAL-ZLXZ202008048.htm
    [11]
    方燕, 郑旭旭, 李丽燕. MiR-146b调控甲状腺乳头状癌细胞生物学行为的机制研究[J]. 中华内分泌外科杂志, 2022, 16(1): 58-63.
    [12]
    刘培发, 侯文宇, 菅雁兵, 等. 甲状腺癌组织中微小RNA-495-3p、Holliday交叉识别蛋白表达水平及临床意义[J]. 实用临床医药杂志, 2022, 26(2): 46-51. doi: 10.7619/jcmp.20213538
    [13]
    SUN W B, MA M N, YU H M, et al. Inhibition of lncRNA X inactivate-specific transcript ameliorates inflammatory pain by suppressing satellite glial cell activation and inflammation by acting as a sponge of miR-146a to inhibit Nav 1. 7[J]. J Cell Biochem, 2018, 119(12): 9888-9898. doi: 10.1002/jcb.27310
    [14]
    游进, 张佳, 徐飞, 等. 下调长链非编码RNAHOXA-AS3靶向促进微小RNA-29b对结肠癌细胞LOVO凋亡和奥沙利铂敏感性的影响[J]. 实用临床医药杂志, 2022, 26(2): 69-75. doi: 10.7619/jcmp.20213051
    [15]
    WU F, LI F, LIN X, et al. Exosomes increased angiogenesis in papillary thyroid cancer microenvironment[J]. Endocr Relat Cancer, 2019, 26(5): 525-538.
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