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XIONG Huazhao, YAN Mingquan, XIAO Weiming, ZHANG Siqin. Differential expression and core genes mining of mitophagy-related genes in stomach adenocarcinoma[J]. Journal of Clinical Medicine in Practice, 2023, 27(19): 1-6, 11. DOI: 10.7619/jcmp.20231748
Citation: XIONG Huazhao, YAN Mingquan, XIAO Weiming, ZHANG Siqin. Differential expression and core genes mining of mitophagy-related genes in stomach adenocarcinoma[J]. Journal of Clinical Medicine in Practice, 2023, 27(19): 1-6, 11. DOI: 10.7619/jcmp.20231748
shu

Differential expression and core genes mining of mitophagy-related genes in stomach adenocarcinoma

  • 1.

    Medical College of Yangzhou University, Yangzhou, Jiangsu, 225000

  • 2.

    Department of Gastroenterology, the Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, 225001

More Information
  • Received Date: May 29, 2023
  • Revised Date: July 20, 2023
  • Available Online: October 18, 2023
    Graphical Abstract
    • Abstract
  • Objective 

    To explore differential expression of mitophagy-related genes (MRGs) in stomach adenocarcinoma(STAD) and to investigate related core genes mining.

    Methods 

    Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases to obtain clinical sample information of patients with STAD. A total of 26 MRGs with statistical significance were obtained based on the Gene Set Enrichment Analysis (GSEA) website and GeneCards database. A prognostic model of MRGs in STAD was constructed based on Least Absolute Shrinkage and Selection Operator (LASSO) regression, and mitochondrial autophagy prognostic genes (MPRGs) were screened out. Through gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, key genes involved in biological processes and pathways were obtained. Protein-protein interaction(PPI) network was established by means of STRING database, and the Cytoscape software was utilized for visualization.

    Results 

    Fifteen MRGs were selected in the LASSO regression model: ATG12, CSNK2A2, CSNK2B, FUNDC1, MAP1LC3A, MAP1LC3B, PGAM5, PINK1, SQSTM1, TOMM20, TOMM22, TOMM40, TOMM5, UBA52 and UBC, which were risk factors for STAD. Among 15 MPRGs, UBC and UBA52 genes had more impact on progression and prognosis of STAD, and the expression of PGAM5 was significantly correlated with the occurrence of STAD. ATG12 gene had the highest functional similarity score with other genes. PPI network analysis showed that PINK1 protein had the most interactions with other proteins.

    Conclusion 

    A total of 15 MPRGs play important roles in the occurrence and development of STAD, and may be used as targets for STAD gene detection, treatment and independent prognostic tools for STAD.

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    • References (25)
  • [1]
    SHAFABAKHSH R, YOUSEFI B, ASEMI Z, et al. Chitosan: a compound for drug delivery system in gastric cancer-a review[J]. Carbohydr Polym, 2020, 242: 116403. doi: 10.1016/j.carbpol.2020.116403
    [2]
    钱慧琴. 基于网络药理学探讨肿节风治疗胃癌的作用机制[J]. 沈阳药科大学学报, 2022, 39(8): 979-987. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYD202208012.htm
    [3]
    AVENTAGGIATO M, VERNUCCI E, BARRECA F, et al. Sirtuins' control of autophagy and mitophagy in cancer[J]. Pharmacol Ther, 2021, 221: 107748. doi: 10.1016/j.pharmthera.2020.107748
    [4]
    李祎龙, 雷凯, 张小昭, 等. 1, 25-二羟维生素D3对人结肠癌细胞凋亡和自噬的影响及机制研究[J]. 实用临床医药杂志, 2021, 25(13): 5-9. doi: 10.7619/jcmp.20211388
    [5]
    FERRO F, SERVAIS S, BESSON P, et al. Autophagy and mitophagy in cancer metabolic remodelling[J]. Semin Cell Dev Biol, 2020, 98: 129-138. doi: 10.1016/j.semcdb.2019.05.029
    [6]
    张标. TP53突变在肝细胞癌中的意义[D]. 大连: 大连医科大学, 2022.
    [7]
    YUAN Q H, DENG D W, PAN C, et al. Integration of transcriptomics, proteomics, and metabolomics data to reveal HER2-associated metabolic heterogeneity in gastric cancer with response to immunotherapy and neoadjuvant chemotherapy[J]. Front Immunol, 2022, 13: 951137. doi: 10.3389/fimmu.2022.951137
    [8]
    GOLDMAN M J, CRAFT B, HASTIE M, et al. Visualizing and interpreting cancer genomics data via the Xena platform[J]. Nat Biotechnol, 2020, 38(6): 675-678. doi: 10.1038/s41587-020-0546-8
    [9]
    张良舜, 叶桦. 基于GEO胃癌芯片数据的生物信息学分析[J]. 现代实用医学, 2022, 34(11): 1409-1411, F0002. doi: 10.3969/j.issn.1671-0800.2022.11.004
    [10]
    唐焘, 武敏, 王君, 等. C型尼曼-匹克蛋白2在胃癌组织中的表达及其功能初步研究[J]. 第三军医大学学报, 2021, 43(21): 2381-2388. https://www.cnki.com.cn/Article/CJFDTOTAL-DSDX202121017.htm
    [11]
    蒿花, 田国祥, 耿辉, 等. 人类基因综合分析数据库GeneCards的应用介绍[J]. 中国循证心血管医学杂志, 2021, 13(8): 902-906. doi: 10.3969/j.issn.1674-4055.2021.08.03
    [12]
    葛继芸, 姚晨, 张菁, 等. CALB2介导的肿瘤微环境免疫细胞浸润在肝癌复发中的作用机制分析[J]. 海军军医大学学报, 2022, 43(7): 744-751. https://www.cnki.com.cn/Article/CJFDTOTAL-DEJD202207004.htm
    [13]
    桑珍珍, 杨栋梁, 饶欣, 等. 基于加权基因共表达网络分析探讨新型冠状病毒感染相关脓毒症潜在的关键基因[J]. 中国急救医学, 2023, 43(5): 376-382. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJJY202305007.htm
    [14]
    何融泉. 大数据先验模式构建膀胱癌非编码RNA全景图谱及SCARNA12的分子机制研究[D]. 南宁: 广西医科大学, 2019.
    [15]
    吴茜, 宋兴勃, 钟慧钰, 等. 胃癌关键基因和通路的生物信息学和功能分析[J]. 肿瘤预防与治疗, 2020, 33(2): 131-139. https://www.cnki.com.cn/Article/CJFDTOTAL-SCZF202002009.htm
    [16]
    姚志强. 基于生物信息学鉴定CTSV作为膀胱癌诊断和预后的标志物及预后nomogram的构建[D]. 兰州: 兰州大学, 2021.
    [17]
    罗倩, 詹雪冰, 董芳媛, 等. 基于数据库分析CDCA基因家族在胃癌中的表达及预后意义[J]. 包头医学院学报, 2021, 37(11): 45-50. https://www.cnki.com.cn/Article/CJFDTOTAL-BTYX202111011.htm
    [18]
    张颖, 邱汉波, 侯恩存, 等. 基于网络药理学-分子对接研究益气逐淤汤针对胃癌的作用机制[J]. 世界中医药, 2021, 16(23): 3484-3490. https://www.cnki.com.cn/Article/CJFDTOTAL-SJZA202123014.htm
    [19]
    NIE Y L, LIANG X J, LIU S H, et al. WASF3 knockdown sensitizes gastric cancer cells to oxaliplatin by inhibiting ATG12-mediated autophagy[J]. Am J Med Sci, 2020, 359(5): 287-295.
    [20]
    MENG L, HU P B, XU A M. PGAM5 promotes tumorigenesis of gastric cancer cells through PI3K/AKT pathway[J]. Pathol Res Pract, 2023, 244: 154405.
    [21]
    张浩, 张悦, 赵文武, 等. PINK1/Parkin介导的线粒体自噬及其在肝脏疾病发生发展中的作用机制[J]. 临床肝胆病杂志, 2020, 36(7): 1663-1665. https://www.cnki.com.cn/Article/CJFDTOTAL-LCGD202007057.htm
    [22]
    肖依依. 二甲双胍通过激活AMPKα诱导PINK1/Parkin介导的线粒体自噬降低胃癌细胞顺铂化疗敏感性的研究[D]. 南昌: 南昌大学, 2022.
    [23]
    SALAZAR C, RUIZ-HINCAPIE P, RUIZ L. The interplay among PINK1/PARKIN/dj-1 network during mitochondrial quality control in cancer biology: protein interaction analysis[J]. Cells, 2018, 7(10): 154.
    [24]
    MIYAZAKI N, SHIRATORI R, OSHIMA T, et al. PINK1-dependent and Parkin-independent mitophagy is involved in reprogramming of glycometabolism in pancreatic cancer cells[J]. Biochem Biophys Res Commun, 2022, 625: 167-173.
    [25]
    李娜, 王培红, 李俊杰, 等. MIF通过调节急性幽门螺杆菌感染介导的炎症反应、自噬和凋亡促进胃癌细胞增殖及细胞周期进展[J]. 中国免疫学杂志, 2023, 39(2): 336-342. https://www.cnki.com.cn/Article/CJFDTOTAL-ZMXZ202302019.htm
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