Relationships of expression of high mobility group box 1 protein and toll-like receptor 4 in patients with clinical characteristics of refractory epilepsy and their predictive value

WANG Yu; YANG Jixue; GUO Dianqu; LIU Wenbin

doi:10.7619/jcmp.20241965

Journal of Clinical Medicine in Practice > 2024 > 28(19): 68-72, 83. > DOI: 10.7619/jcmp.20241965

WANG Yu, YANG Jixue, GUO Dianqu, LIU Wenbin. Relationships of expression of high mobility group box 1 protein and toll-like receptor 4 in patients with clinical characteristics of refractory epilepsy and their predictive value[J]. Journal of Clinical Medicine in Practice, 2024, 28(19): 68-72, 83. DOI: 10.7619/jcmp.20241965

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Relationships of expression of high mobility group box 1 protein and toll-like receptor 4 in patients with clinical characteristics of refractory epilepsy and their predictive value

Department of Neurosurgery, the First People's Hospital of Zhengzhou of Henan Province, Zhengzhou, Henan, 450000

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Received Date: May 12, 2024
Revised Date: July 16, 2024

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Abstract

Abstract

Objective
To investigate the correlations of the expression of high mobility group box 1 protein (HMGB1) and toll-like receptor 4 (TLR4) with the clinical characteristics of patients with refractory epilepsy, as well as their predictive value.
Methods
A retrospective analysis was conducted on the clinical data of 84 patients with refractory epilepsy, and they were included in observation group. During the same period, 35 patients with intracranial hypertension undergoing craniectomy for decompression were selected as control group. Immunohistochemistry was used to detect the expressions of HMGB1 and TLR4, while Nissl staining was applied to observe tissue morphology. The relevant experimental results were analyzed simultaneously.
Results
The expression levels of HMGB1 and TLR4 in the observation group were significantly higher than those in the control group (P < 0.05). The optical density values of HMGB1 and TLR4 protein bands as well as internal reference β-actin bands in the observation group were significantly higher than those of the control group (P < 0.05). The intensity of TLR4 expression was correlated with seizure frequency, seizure duration, disease duration, and seizure type, while the intensity of HMGB1 expression was correlated with seizure frequency, seizure duration, and disease duration. In the lesion tissues, the expression of TLR4 was positively correlated with the expression of HMGB1, suggesting a synergistic effect between them. Receiver Operating Characteristic (ROC) curve analysis revealed that the areas under the curve (AUCs) for TLR4 and HMGB1 proteins in predicting refractory epilepsy were 0.888 and 0.923, respectively.
Conclusion
HMGB1 and TLR4 are highly expressed in the lesions of patients with refractory epilepsy, and their expression intensities are positively correlated. They can predict the occurrence and development of the disease to a certain extent, providing new biomarkers for the diagnosis and treatment of refractory epilepsy.
- toll-like receptor 4,
- high mobility group box 1 protein,
- prediction,
- refractory epilepsy,
- correlation,
- retrospective analysis

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References (15)

References

[1]	FEJA M, MELLER S, DEKING L S, et al. OV329, a novel highly potent γ-aminobutyric acid aminotransferase inactivator, induces pronounced anticonvulsant effects in the pentylenetetrazole seizure threshold test and in amygdala-kindled rats[J]. Epilepsia, 2021, 62(12): 3091-3104. doi: 10.1111/epi.17090
[2]	李承俊, 王丰, 姚培森, 等. 局灶性皮质发育不良相关"难定位" 难治性癫痫的手术疗效及影响因素分析[J]. 中华神经医学杂志, 2021, 20(8): 793-798. doi: 10.3760/cma.j.cn115354-20201210-00959
[3]	姚娟, 钱忆家, 翁柠, 等. 平肝止痫复方联合卡马西平干预HMGB1/TLR4信号炎症通路的相关作用研究[J]. 辽宁中医杂志, 2022, 49(2): 191-198, 后插8.
[4]	陈小妮, 谭会会, 殷艳玲, 等. 原发性癫痫患者血清BAFF、HMGB1、TLR4的表达水平及其临床意义[J]. 海南医学, 2023, 34(4): 550-553.
[5]	中国医师协会神经内科医师分会癫痫疾病专业委员会. 中国基因性全面性癫痫临床诊治实践指南[J]. 中华神经医学杂志, 2020, 19(10): 973-976. doi: 10.3760/cma.j.cn115354-20200529-00424
[6]	WATSON G D R, AFRA P, BARTOLINI L, et al. A journey into the unknown: an ethnographic examination of drug-resistant epilepsy treatment and management in the United States[J]. Epilepsy Behav, 2021, 124: 108319. doi: 10.1016/j.yebeh.2021.108319
[7]	MCALLISTER-WILLIAMS R H, BULMER S, NEWTON K, et al. Assessment for vagus nerve stimulation in patients with difficult-to-treat depression: a model from the Newcastle Regional Affective Disorders Service (RADS)[J]. J Affect Disord, 2021, 280(Pt A): 315-318.
[8]	季涛云, 王若凡, 刘庆祝, 等. 局灶起源的药物难治性癫痫性痉挛的手术预后及相关预后因素分析[J]. 中华实用儿科临床杂志, 2021, 36(17): 1333-1337. doi: 10.3760/cma.j.cn101070-20200505-00769
[9]	李冬梅, 陈巧彬, 陈琅, 等. FPS-ZM1和mNGF对癫痫幼鼠脑内HMGB1和TRX表达的影响[J]. 福建医科大学学报, 2021, 55(6): 513-516. doi: 10.3969/j.issn.1672-4194.2021.06.010
[10]	黄亮, 张思磊, 朱作磊, 等. 微小RNA-542-3p和Toll样受体4在癫痫患者血浆中的表达及临床意义[J]. 脑与神经疾病杂志, 2022, 30(4): 241-245.
[11]	李东秀, 潘彩芬. 血清高迁移率族蛋白B1水平与小儿热性惊厥临床特征及转为癫痫的相关性[J]. 临床和实验医学杂志, 2021, 20(9): 927-931. doi: 10.3969/j.issn.1671-4695.2021.09.009
[12]	屈会霞, 袁小锋, 屈昕. 长链非编码RNA核富含丰富的转录本1对癫痫模型海马神经元凋亡的影响和作用机制[J]. 中国儿童保健杂志, 2021, 29(11): 1193-1197, 1212. doi: 10.11852/zgetbjzz2021-0402
[13]	黄凌锋, 廉荣镇, 肖能, 等. 癫痫患儿血清α-突触核蛋白和热休克蛋白70的表达及临床意义[J]. 国际检验医学杂志, 2021, 42(13): 1545-1548, 1553. doi: 10.3969/j.issn.1673-4130.2021.13.003
[14]	李倩, 顾晶晶. 成年难治性癫痫患者行激光间质热疗术后疗效观察与护理[J]. 护士进修杂志, 2022, 37(11): 1043-1045.
[15]	金超, 董琰, 张双, 等. 药物联合方案治疗小儿癫痫临床疗效及其对高迁移率族蛋白-1、Toll样受体4水平影响[J]. 临床军医杂志, 2022, 50(6): 636-638, 641.

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Relationships of expression of high mobility group box 1 protein and toll-like receptor 4 in patients with clinical characteristics of refractory epilepsy and their predictive value