Citation: | WANG Lin, LIU Bing, LIU Guohua, YANG Hanping. Correlation between resistin gene polymorphism and colorectal cancer susceptibility[J]. Journal of Clinical Medicine in Practice, 2023, 27(2): 50-54. DOI: 10.7619/jcmp.20222617 |
To investigate the relationship between resistin gene polymorphism and colorectal cancer susceptibility.
A total of 56 patients with colorectal cancer were selected as study subjects (case group). Fifty-two patients with colorectal adenoma were selected as control group. During the same period, 60 healthy subjects were selected as healthy group. The serum genomic DNA of the three groups was extracted, rs1862513 fragment of resistin gene was amplified by polymerase chain reaction(PCR) and was sequenced, and its gene polymorphism was explored; the levels of resistin, adiponectin and leptin were detected by enzyme-linked immunosorbent assay (ELISA); the genotype distribution of rs1862513 was compared among the three groups; Logistic regression analysis was used to analyze the factors affecting the occurrence of colorectal adenocarcinoma.
There were three genotypes of resistin gene at rs1862513 locus: GG, GC and CC genotypes. In the healthy group, GG genotype was the most, CC genotype was the least; in the control group, GC genotype was the most, CC genotype was the least; in the case group, CC genotype was the most, GG genotype was the least. The each genotype in case group, control group and healthy group were in accordance with Hardy-Weinberg equilibrium law (P>0.05). The level of resistin in the case group was significantly lower than that in the healthy group and the control group, and the level of adiponectin and leptin was significantly higher than those in the healthy group and the control group (P < 0.05); the level of resistin in the control group was significantly lower than that in the healthy group, and the levels of adiponectin and leptin were significantly higher than those in the healthy group (P < 0.05). The level of serum resistin of CC type colorectal cancer patients was significantly lower than that of GG type and GC type, and the levels of adiponectin and leptin were significantly higher than that of GG type and GC type (P < 0.05); the level of serum resistin of GC type colorectal cancer patients was significantly lower than that of GG type, and the levels of adiponectin and leptin were significantly higher than that of GG type (P < 0.05). The CC genotype was an independent risk factor for colorectal cancer (P < 0.05).
Resistin CC genotype is associated with colorectal cancer susceptibility, and may provide potential genetic markers for diagnosis and treatment of colorectal cancer patients.
[1] |
MAHMOOD S, ENGLISH D R, MACINNIS R J, et al. Domain-specific physical activity and the risk of colorectal cancer: results from the Melbourne Collaborative Cohort Study[J]. BMC Cancer, 2018, 18(1): 1063. doi: 10.1186/s12885-018-4961-x
|
[2] |
王征新. 腹腔镜结直肠癌根治术治疗结直肠癌降低术中出血量和术后并发症发生率的效果研究[J]. 中国药物与临床, 2019, 19(8): 1313-1315. https://www.cnki.com.cn/Article/CJFDTOTAL-YWLC201908046.htm
|
[3] |
JOSHI A, MADDIPATI S, CHATTERJEE A, et al. Gingival crevicular fluid resistin levels in chronic periodontitis with type 2 diabetes before and after non-surgical periodontal therapy: a clinico-biochemical study[J]. Indian J Dent Res, 2019, 30(1): 47-51.
|
[4] |
LI H M, ZHANG T P, LI X M, et al. Association of single nucleotide polymorphisms in resistin gene with rheumatoid arthritis in a Chinese population[J]. J Clin Lab Anal, 2018, 32(9): e22595.
|
[5] |
FARAHANI H, MAHMOUDI T, ASADI A, et al. Insulin resistance and colorectal cancer risk: the role of elevated plasma resistin levels[J]. J Gastrointest Cancer, 2020, 51(2): 478-483. doi: 10.1007/s12029-019-00260-7
|
[6] |
顾晋, 汪建平, 孙燕, 等. 中国结直肠癌诊疗规范(2017年版)[J]. 中华临床医师杂志: 电子版, 2018, 12(1): 3-23. doi: 10.3877/cma.j.issn.1674-0785.2018.01.003
|
[7] |
胡燕菲, 詹利, 郑立东. 静脉全身麻醉分别联合硬膜外麻醉与超声引导双侧腹横肌平面对腹腔镜结直肠癌手术患者的影响[J]. 实用临床医药杂志, 2022, 26(6): 100-104. doi: 10.7619/jcmp.20214140
|
[8] |
LIU L B, LIU T, XIN F Z. Correlations of ICAM-1 gene polymorphisms with susceptibility and multidrug resistance in colorectal cancer in a Chinese population[J]. Medicine, 2017, 96(33): e7481. doi: 10.1097/MD.0000000000007481
|
[9] |
屠海明. 结肠癌相关因素和DNA甲基转移酶1基因多态性与结肠癌易感性的相关性分析[J]. 医学临床研究, 2018, 35(9): 1727-1730. doi: 10.3969/j.issn.1671-7171.2018.09.020
|
[10] |
王娜, 董燕, 滑芳, 等. 新生儿血清中抵抗素与产妇脂联素、瘦素及糖代谢及妊娠糖尿病的关系[J]. 中国卫生检验杂志, 2018, 28(2): 184-185, 196. https://www.cnki.com.cn/Article/CJFDTOTAL-ZWJZ201802019.htm
|
[11] |
徐滨华, 李征寒, 王立平, 等. 不同脂肪分层对肥胖2型糖尿病抵抗素、内脂素水平影响[J]. 中国卫生标准管理, 2018, 9(11): 26-28. https://www.cnki.com.cn/Article/CJFDTOTAL-WSBZ201811013.htm
|
[12] |
DE LUIS D A, IZAOLA O, PRIMO D, et al. Effect of the rs1862513 variant of resistin gene on insulin resistance and resistin levels after two hypocaloric diets with different fat distribution in subjects with obesity[J]. Eur Rev Med Pharmacol Sci, 2018, 22(12): 3865-3872.
|
[13] |
SHEHATA W A, MARAEE A, WAHAB T A A, et al. Serum resistin levels and resistin gene polymorphism in patients with acne vulgaris: does it correlate with disease severity[J]. Int J Dermatol, 2021, 60(10): 1270-1277. doi: 10.1111/ijd.15727
|
[14] |
HASHEMI M, BAHARI G, TABASI F, et al. Association between rs1862513 and rs3745367 genetic polymorphisms of resistin and risk of cancer: a meta-analysis[J]. Asian Pac J Cancer Prev, 2018, 19(10): 2709-2716.
|
[15] |
罗俊, 徐芬, 詹媛丽, 等. Resistin基因多态性对早产儿坏死性小肠结肠炎易感性的影响[J]. 中国妇幼卫生杂志, 2019, 10(5): 25-29, 40. https://www.cnki.com.cn/Article/CJFDTOTAL-YZWS201905004.htm
|
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