Correlation between resistin gene polymorphism and colorectal cancer susceptibility

Objective To investigate the relationship between resistin gene polymorphism and colorectal cancer susceptibility.

Methods A total of 56 patients with colorectal cancer were selected as study subjects (case group). Fifty-two patients with colorectal adenoma were selected as control group. During the same period, 60 healthy subjects were selected as healthy group. The serum genomic DNA of the three groups was extracted, rs1862513 fragment of resistin gene was amplified by polymerase chain reaction(PCR) and was sequenced, and its gene polymorphism was explored; the levels of resistin, adiponectin and leptin were detected by enzyme-linked immunosorbent assay (ELISA); the genotype distribution of rs1862513 was compared among the three groups; Logistic regression analysis was used to analyze the factors affecting the occurrence of colorectal adenocarcinoma.

Results There were three genotypes of resistin gene at rs1862513 locus: GG, GC and CC genotypes. In the healthy group, GG genotype was the most, CC genotype was the least; in the control group, GC genotype was the most, CC genotype was the least; in the case group, CC genotype was the most, GG genotype was the least. The each genotype in case group, control group and healthy group were in accordance with Hardy-Weinberg equilibrium law (P>0.05). The level of resistin in the case group was significantly lower than that in the healthy group and the control group, and the level of adiponectin and leptin was significantly higher than those in the healthy group and the control group (P < 0.05); the level of resistin in the control group was significantly lower than that in the healthy group, and the levels of adiponectin and leptin were significantly higher than those in the healthy group (P < 0.05). The level of serum resistin of CC type colorectal cancer patients was significantly lower than that of GG type and GC type, and the levels of adiponectin and leptin were significantly higher than that of GG type and GC type (P < 0.05); the level of serum resistin of GC type colorectal cancer patients was significantly lower than that of GG type, and the levels of adiponectin and leptin were significantly higher than that of GG type (P < 0.05). The CC genotype was an independent risk factor for colorectal cancer (P < 0.05).

Conclusion Resistin CC genotype is associated with colorectal cancer susceptibility, and may provide potential genetic markers for diagnosis and treatment of colorectal cancer patients.