Effects of calycosin on proliferation and apoptosis of HeLa cells of cervical cancer

HUANG Lili; ZHANG Zhiqin; HUANG Qi; SU Ruilian; HUANG Xiumin

doi:10.7619/jcmp.20232234

Journal of Clinical Medicine in Practice > 2023 > 27(18): 5-10. > DOI: 10.7619/jcmp.20232234

HUANG Lili, ZHANG Zhiqin, HUANG Qi, SU Ruilian, HUANG Xiumin. Effects of calycosin on proliferation and apoptosis of HeLa cells of cervical cancer[J]. Journal of Clinical Medicine in Practice, 2023, 27(18): 5-10. DOI: 10.7619/jcmp.20232234

Citation:

PDF (2292 KB)

Effects of calycosin on proliferation and apoptosis of HeLa cells of cervical cancer

1.
Department of Gynecology and Obstetrics, ZhongShan Hospital Affiliated to Xiamen University, Xiamen, Fujian, 361004
2.
School of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian, 350000

More Information

Received Date: July 13, 2023
Revised Date: September 05, 2023
Available Online: October 08, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effect of calycosin on proliferation and apoptosis of HeLa cells of cervical cancer and its possible mechanism.
Methods
Human cervical cancer HeLa cells were cultured in vitro and divided into control group (without treatment with calycosin), calycosin-low group (25 μmol/L), calycosin-medium group (50 μmol/L) and calycosin-high group (100 μmol/L); the cells were cultured with 100 μmol/L of calycosin for 24 h, and were divided into microRNA (miR)-NC group, miR-4766-5p group, calycosin+anti-miR-NC group, and calycosin+anti-miR-4766-5p group, respectively. Cell proliferation, clonal formation and apoptosis were detected by CCK-8 assay, plate cloning assay and flow cytometry; real-time quantitative fluorescent polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression levels of miR-4766-5p and apoptosis-related proteins, respectively.
Results
Compared with the control group, cell proliferation was significantly inhibited, apoptosis-protein content and miR-4766-5p expression were significantly increased in a dose-dependent manner in the calycosin-low group, calycosin-medium group and calycosin-high group (P < 0.05). The expression level of miR-4766-5p in cervical cancer tissues was significantly decreased compared with paracancer tissues (P < 0.05). Compared with the miR-NC group, the cell proliferation capacity of the miR-4766-5p group was significantly decreased, and the apoptotic protein content was significantly increased (P < 0.05). The cell proliferation inhibition rate, apoptosis rate and cleaved-caspase3 and cleaved-caspase9 protein levels in the calycosin+anti-miR-4766-5p group were significantly lower than those in the calycosin+anti-miR-NC group, the increase of cell clonogenesis ability was significantly higher than that of the calycosin+anti-miR-NC group (P < 0.05).
Conclusion
Calycosin can inhibit the proliferation of cervical cancer cells and induce cell apoptosis by up-regulating the expression of miR-4766-5p.
- cervical cancer,
- calycosin,
- microRNA-4766-5p,
- cell proliferation,
- apoptosis

FullText(HTML)

References (22)

References

[1]	LIU S Y, YAN H, LIU Y, et al. Zhidai Decoction inhibits cervical cancer through regulation of vaginal microbiota[J]. Evid Based Complement Alternat Med, 2020, 2020: 8940582.
[2]	LIU Y M, GAO S J, ZHU J, et al. Dihydroartemisinin induces apoptosis and inhibits proliferation, migration, and invasion in epithelial ovarian cancer via inhibition of the hedgehog signaling pathway[J]. Cancer Med, 2018, 7(11): 5704-5715. doi: 10.1002/cam4.1827
[3]	LIU L P, SUN L, ZHENG J, et al. Berberine modulates Keratin 17 to inhibit cervical cancer cell viability and metastasis[J]. J Recept Signal Transduct Res, 2021, 41(6): 521-531. doi: 10.1080/10799893.2020.1830110
[4]	XIA C L, HE Z H, CAI Y T. Quantitative proteomics analysis of differentially expressed proteins induced by astragaloside Ⅳ in cervical cancer cell invasion[J]. Cell Mol Biol Lett, 2020, 25: 25. doi: 10.1186/s11658-020-00218-9
[5]	辛敏, 陈健, 郭艳红. 毛蕊异黄酮对膀胱癌细胞凋亡和PI3K/Akt信号通路的影响[J]. 广东医学, 2015, 36(5): 690-693. doi: 10.13820/j.cnki.gdyx.20150313.014
[6]	ZHAN W, LIAO X, CHEN Z S, et al. LINC00858 promotes colorectal cancer by sponging miR-4766-5p to regulate PAK2[J]. Cell Biol Toxicol, 2020, 36(4): 333-347. doi: 10.1007/s10565-019-09506-3
[7]	WEI Y N, WANG Y N, ZANG A M, et al. miR-4766-5p inhibits the development and progression of gastric cancer by targeting NKAP[J]. Onco Targets Ther, 2019, 12: 8525-8536. doi: 10.2147/OTT.S220234
[8]	ZHOU P L, WU Z Y, ZHANG W G, et al. Circular RNA hsa_circ_0000277 sequesters miR-4766-5p to upregulate LAMA1 and promote esophageal carcinoma progression[J]. Cell Death Dis, 2021, 12(7): 676. doi: 10.1038/s41419-021-03911-5
[9]	曾文郸, 苗慧, 任倩瑶. 毛蕊异黄酮对人乳腺癌细胞增殖及凋亡的作用及机制研究[J]. 四川生理科学杂志, 2019, 41(2): 95-98. https://www.cnki.com.cn/Article/CJFDTOTAL-SCSZ201902001.htm
[10]	ZHANG H Y, XIE B B, ZHANG Z, et al. Tetrandrine suppresses cervical cancer growth by inducing apoptosis in vitro and in vivo[J]. Drug Des Devel Ther, 2019, 13: 119-127.
[11]	SUN X D, XU Q Q, ZENG L, et al. Resveratrol suppresses the growth and metastatic potential of cervical cancer by inhibiting STAT3^Tyr705 phosphorylation[J]. Cancer Med, 2020, 9(22): 8685-8700. doi: 10.1002/cam4.3510
[12]	LIU S, WANG H Y, MU J, et al. MiRNA-211 triggers an autophagy-dependent apoptosis in cervical cancer cells: regulation of Bcl-2[J]. Naunyn Schmiedebergs Arch Pharmacol, 2020, 393(3): 359-370. doi: 10.1007/s00210-019-01720-4
[13]	REN Y F, DONG J, HE P Y, et al. miR-587 promotes cervical cancer by repressing interferon regulatory factor 6[J]. J Gene Med, 2020, 22(11): e3257. doi: 10.1002/jgm.3257
[14]	刘彬, 刘慰华, 张竞之, 等. 毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响[J]. 贵阳医学院学报, 2014, 39(2): 162-166. https://www.cnki.com.cn/Article/CJFDTOTAL-GYYB201402006.htm
[15]	周立霞, 关洪全, 马贤德, 等. 毛蕊异黄酮对肺腺癌细胞增殖和迁移功能的影响[J]. 辽宁中医杂志, 2019, 46(7): 1474-1477, 1567. https://www.cnki.com.cn/Article/CJFDTOTAL-LNZY201907044.htm
[16]	TIAN J, WANG Y, ZHANG X, et al. Calycosin inhibits the in vitro and in vivo growth of breast cancer cells through WDR7-7-GPR30 Signaling[J]. J Exp Clin Cancer Res, 2017, 36(1): 153. doi: 10.1186/s13046-017-0625-y
[17]	ZHANG Z X, LIN M, WANG J L, et al. Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1[J]. Aging, 2021, 13(12): 16009-16023.
[18]	ZHANG Y, ZHANG J Q, ZHANG T, et al. Calycosin induces gastric cancer cell apoptosis via the ROS-mediated MAPK/STAT3/NF-κB pathway[J]. Onco Targets Ther, 2021, 14: 2505-2517.
[19]	TWIDDY D, CAIN K. Caspase-9 cleavage, do You need it[J]. Biochem J, 2007, 405(1): e1-e2.
[20]	ZHANG J, YANG S P, WANG K N, et al. Crocin induces autophagic cell death and inhibits cell invasion of cervical cancer SiHa cells through activation of PI3K/AKT[J]. Ann Transl Med, 2020, 8(18): 1180.
[21]	PENG L P, CHEN Z Z, WANG G C, et al. Long noncoding RNA LBX2-AS1-modulated miR-4766-5p regulates gastric cancer development through targeting CXCL5[J]. Cancer Cell Int, 2020, 20: 497.
[22]	CHEN H, WU C, LUO L, et al. circ_0000467 promotes the proliferation, metastasis, and angiogenesis in colorectal cancer cells through regulating KLF12 expression by sponging miR-4766-5p[J]. Open Med, 2021, 16(1): 1415-1427.

[1]	YANG Junxia. Effect of rehabilitation nursing intervention on the improvement of quality of life in patients with cerebral infarction[J]. Journal of Clinical Medicine in Practice, 2019, 23(12): 96-98, 102. DOI: 10.7619/jcmp.201912028
[2]	JIANG Rongfei, XU Ling, LI Xiaohua. Influence of pulmonary rehabilitation training combined with seretide and tiotropium bromide on pulmonary function, exercise endurance and quality of life in patients with moderate and severe COPD[J]. Journal of Clinical Medicine in Practice, 2017, (9): 28-30,34. DOI: 10.7619/jcmp.201709007
[3]	CHENG Hong, TANG Yong, HU Bin. Effect of high quality nursing on quality of life in elderly patients with chronic bronchitis[J]. Journal of Clinical Medicine in Practice, 2016, (8): 37-39. DOI: 10.7619/jcmp.201608012
[4]	Influence of counterpart nursing on the pain and life quality of patients with gout[J]. Journal of Clinical Medicine in Practice, 2016, (6): 35-38. DOI: 10.7619/jcmp.201606012
[5]	FAN Wenxia, XIAO Qiong, ZHOU Ke. Clinical observation on the effect of nursing intervention on psychologyand quality of life in patients with hemodialysis[J]. Journal of Clinical Medicine in Practice, 2015, (14): 36-38. DOI: 10.7619/jcmp.201514012
[6]	YANG Xiaoping, CHEN Qinjie, LI Lina, CHEN Qing. Quality of life in infertile couples and its influencing factors[J]. Journal of Clinical Medicine in Practice, 2015, (10): 71-74. DOI: 10.7619/jcmp.201510022
[7]	XIANG Mei, LIU Aihua. The impact of high quality nursing on adverse emotion and quality of life in patients with colorectal cancer after operation[J]. Journal of Clinical Medicine in Practice, 2015, (6): 62-64. DOI: 10.7619/jcmp.201506020
[8]	WANG Mei, LIU Falan. The influence of self-management guidance on the hope level and life quality in COPD patients[J]. Journal of Clinical Medicine in Practice, 2014, (12): 112-114. DOI: 10.7619/jcmp.201412038
[9]	DING Jiehong. The effect of evidence-based nursing on life quality of gastric ulcer patients[J]. Journal of Clinical Medicine in Practice, 2014, (10): 89-90,93. DOI: 10.7619/jcmp.201410031
[10]	SONG Wenying. Influence of nursing intervention on the quality of life of patients with depression[J]. Journal of Clinical Medicine in Practice, 2013, (16): 147-149. DOI: 10.7619/jcmp.201316057

Cited By

Cited by

Periodical cited type(7)

1.	付政平，董贤明，秦建领，卢奎彬，渠展，王庆丰，汤聪，徐红艳，岳雨倩. 社区获得性肺炎凝血功能异常对治疗和预后的影响. 山西卫生健康职业学院学报. 2024(06): 40-42 .
2.	叶文，龙尧水，陈咏梅，胡泽玉，白文学. MPV、PCT、PDW、P-LCR、MPR对儿童SCAP的诊断价值. 检验医学与临床. 2023(09): 1325-1329 .
3.	吴颖，高蔚，翁婷. 血浆FIB-PLT-DD联合检测在慢性肺源性心脏病治疗中的临床意义. 中国医药科学. 2023(19): 177-180+185 .
4.	史妹，周历安，符叶柳. 床旁肺超声B线计数及外周血嗜酸性粒细胞评估慢性阻塞性肺疾病并发肺炎的价值及其相关因素分析. 海军医学杂志. 2023(12): 1243-1247 .
5.	雷云飞，宋影，王亚冰. D–二聚体及凝血功能指标对慢性阻塞性肺疾病的检验价值. 深圳中西医结合杂志. 2022(23): 54-56 .
6.	黄鑫，王卫阳. 阿莫西林克拉维酸钾联合补肺活血胶囊对慢性阻塞性肺疾病患者肺功能及预后影响. 临床军医杂志. 2021(07): 785-786 .
7.	李刚，孙雨婷，王佳美，李满元. 外周血平均血小板体积在儿童社区获得性肺炎中的价值. 中国医药指南. 2021(31): 87-88+91 .

Other cited types(0)

Get Citation

PDF

XML

Article views (141) PDF downloads (13) Cited by(7)

Turn off MathJax

Article Contents

Abstract

References

Effects of calycosin on proliferation and apoptosis of HeLa cells of cervical cancer