A study on fingerprint and common peaks identification of water-soluble components in Bombyx Batryticatus

WANG Siqi; ZHANG Lurong; CHEN Wei; PAN Yunzhi; ZHOU Feng; SU Ziheng; CHENG Junping

doi:10.7619/jcmp.20213009

Journal of Clinical Medicine in Practice > 2021 > 25(23): 53-57. > DOI: 10.7619/jcmp.20213009

WANG Siqi, ZHANG Lurong, CHEN Wei, PAN Yunzhi, ZHOU Feng, SU Ziheng, CHENG Junping. A study on fingerprint and common peaks identification of water-soluble components in Bombyx Batryticatus[J]. Journal of Clinical Medicine in Practice, 2021, 25(23): 53-57. DOI: 10.7619/jcmp.20213009

Citation:

PDF (1592 KB)

A study on fingerprint and common peaks identification of water-soluble components in Bombyx Batryticatus

1.
Suzhou City Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Suzhou, Jiangsu, 215000
2.
the Fifth People's Hospital of Suzhou City in Jiangsu Province, Suzhou, Jiangsu, 215000

More Information

Received Date: July 26, 2021
Available Online: December 20, 2021
Published Date: December 14, 2021

Graphical Abstract

Abstract

Abstract

Objective To qualitatively analyze the water-soluble components in Bombyx Batryticatus by liquid chromatography tandem mass spectrometry (LC-MS/MS) technique and establish the analytical methods of fingerprint and common peaks identification.
Methods The column was Acclaim PA 25 μm C₁₈ column (4.6 mm×250.0 mm), the mobile phase A and B were 0.2% phosphoric acid solution and acetonitrile respectively, the flow rate was 0.2 mL/min, the column temperature was 30℃, and the detection wave length was 265 nm. An electrospray ionization in both negative and positive ion modes was used to detect the molecular weight and fragment molecular of characteristic peaks obtained by LC-MS/MS, and characteristic peaks were identified.
Results A total of 19 common peaks were found in the fingerprints of water-soluble components of Bombyx Batryticatus, and 10 of them were identified by LC-MS/MS. The similarity evaluation results of fingerprints of 10 batches of Bombyx Batryticatus from different producing areas showed that the similarity was between 0.985 and 1.000.
Conclusion In this study, the method of fingerprint analysis based on LC-MS/MS is characterized by high selectivity, specificity and sensitivity, which can provide technical reference for quality evaluation of Bombyx Batryticatus.

FullText(HTML)

References (25)

References

[1]	田蜜, 陈芳, 余坊. 僵蚕的研究进展[J]. 中医药导报, 2015, 21(15): 101-104. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZB201515039.htm
[2]	莫雪林, 胡美变, 肖禾, 等. 僵蚕的本草考证[J]. 中药与临床, 2016, 7(5): 47-50. https://www.cnki.com.cn/Article/CJFDTOTAL-LCZY201605015.htm
[3]	黄居敏, 邓华勇, 蔡英, 等. 白僵蚕化学成分研究[J]. 中草药, 2015, 46(16): 2377-2380. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201516011.htm
[4]	陈文文, 胡美变, 彭伟, 等. 僵蚕中有效成分白僵菌素的研究进展[J]. 中国药房, 2019, 30(24): 3452-3456. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA201924030.htm
[5]	程锁明, 翟银成, 薛芳, 等. 僵蚕化学成分的研究[J]. 中草药, 2015, 46(24): 3630-3636. doi: 10.7501/j.issn.0253-2670.2015.24.003
[6]	黄居敏, 苏明声, 张亚梅, 等. 僵蚕化学成分研究[J]. 中药材, 2017, 40(1): 87-89. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA201701021.htm
[7]	WANG X, ZHANG Y, KONG H, et al. Novel mulberry silkworm cocoon-derived carbon dots and their anti-inflammatory properties[J]. Artif Cells Nanomed Biotechnol, 2020, 48(1): 68-76. doi: 10.1080/21691401.2019.1699810
[8]	ARKOUB-DJERMOUNE L, BOULEKBACHE-MAKHLOUF L, ZEGHICHI-HAMRI S, et al. Influence of the thermal processing on the physico-chemical properties and the antioxidant activity of A Solanaceae vegetable: eggplant[J]. J Food Qual, 2016, 39(3): 181-191. doi: 10.1111/jfq.12192
[9]	胡美变. 麸炒僵蚕的炮制工艺规范化及质量相关研究[D]. 成都: 成都中医药大学, 2017.
[10]	胡文娟, 罗梅兰, 唐亮. 工厂化生产白僵蚕的前景尧存在问题与发展对策[J]. 广东蚕业, 2017, 51(4): 7-9. doi: 10.3969/j.issn.2095-1205.2017.04.06
[11]	柴贝贝, 王莉贞, 李贺, 等. 中药水溶性成分研究进展[J]. 畜牧与饲料科学, 2017, 38(8): 43-47. https://www.cnki.com.cn/Article/CJFDTOTAL-NMXK201708014.htm
[12]	邢东旭, 杨琼, 廖森泰, 等. HPLC指纹图谱法评价市售僵蚕的品质[J]. 蚕业科学, 2018, 44(5): 810-814. https://www.cnki.com.cn/Article/CJFDTOTAL-CYKE201805025.htm
[13]	王更先, 张翠红, 牟志美, 等. 白僵蚕高效液相色谱指纹图谱的研究[J]. 蚕业科学, 2006, 32(1): 74-79. doi: 10.3969/j.issn.0257-4799.2006.01.013
[14]	朱锐灵, 黄佳滢, 刘莹, 等. 僵蚕碱溶性蛋白质的提取工艺优化及指纹图谱分析[J]. 江苏农业科学, 2021, 49(3): 170-175. https://www.cnki.com.cn/Article/CJFDTOTAL-JSNY202103030.htm
[15]	孔凤利, 岳显可, 朱涛, 等. HPLC指纹图谱结合主成分分析研究僵蚕不同溶剂提取物的化学组分[J]. 中国中医药科技, 2017, 24(4): 445-449. https://www.cnki.com.cn/Article/CJFDTOTAL-TJYY201704015.htm
[16]	曾美玲, 沈耐涛, 吴赛伟, 等. 基于UPLC-triple-TOF/MS方法的三叶青化学成分分析[J]. 中草药, 2017, 48(5): 874-883. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201705006.htm
[17]	许友建. 不同白僵蚕代谢物初步比较和质量评价[D]. 合肥: 安徽农业大学, 2017.
[18]	夏倩, 尚强, 张润容, 等. UHPLC-Q-TOF-MS法测定僵蚕及其制剂中白僵菌素[J]. 中成药, 2020, 42(10): 2652-2655. doi: 10.3969/j.issn.1001-1528.2020.10.022
[19]	WANG S, WANG Q, WANG H, et al. Induction of ROS and DNA damage-dependent senescence by icaritin contributes to its antitumor activity in hepatocellular carcinoma cells[J]. Pharm Biol, 2019, 57(1): 424-431. doi: 10.1080/13880209.2019.1628073
[20]	赵清, 秦桂芳, 桂雪虹, 等. 蜜麸炒僵蚕的质量标准研究[J]. 中国实验方剂学杂志, 2013, 19(8): 120-125. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201308035.htm
[21]	李艳, 白明, 宋亚刚, 等. 中药标准汤剂的研究与思考[J]. 中草药, 2018, 49(17): 3977-3980. doi: 10.7501/j.issn.0253-2670.2018.17.001
[22]	饶晓红, 董秀华. 某院2018—2019年度563张门诊中药汤剂处方合理性分析[J]. 抗感染药学, 2021, 18(2): 216-218. https://www.cnki.com.cn/Article/CJFDTOTAL-KGYY202102022.htm
[23]	任海然. 消脓方汤剂汤剂辅助克林霉素对化脓性扁桃体炎患儿的疗效及其对炎性因子水平的影响[J]. 抗感染药学, 2019, 16(10): 1818-1820. https://www.cnki.com.cn/Article/CJFDTOTAL-KGYY201910059.htm
[24]	刘苓娴, 李海波, 石丹枫, 等. UPLC-TQ-MS同时测定金红片中12个成分[J]. 中草药, 2021, 52(12): 3552-3559. doi: 10.7501/j.issn.0253-2670.2021.12.011
[25]	王海波, 李杨. 抗病毒颗粒中芒果苷的高效液相色谱法的含量测定[J]. 抗感染药学, 2020, 17(2): 165-167. https://www.cnki.com.cn/Article/CJFDTOTAL-KGYY202002009.htm

[1]	XU Jiawen, GAO Qing, ZHANG Fei, ZHANG Wen, WANG Yi. Determination of related substances in diazepam rectal gel by high-performance liquid chromatography[J]. Journal of Clinical Medicine in Practice, 2024, 28(17): 92-98. DOI: 10.7619/jcmp.20241171
[2]	SU Qin, WEI Hailin, ZHOU Guohua, ZHANG Ling, ZHANG Pinghu. Lipidomics analysis of medical Marmota himalayana oil[J]. Journal of Clinical Medicine in Practice, 2024, 28(12): 21-25. DOI: 10.7619/jcmp.20233621
[3]	CHEN Chunying, TAO Chao, DING Muzi, WANG Fengwu, ZHU Xiaoli, SHEN Li. Analysis of the chemical constituents in fermentation broth of Myceliophthora lutea ML-1 derived from Concha ostreae based on liquid chromatography tandem mass spectrometry molecular networking[J]. Journal of Clinical Medicine in Practice, 2023, 27(20): 105-112, 117. DOI: 10.7619/jcmp.20231458
[4]	MOU Kai, XU Jia, LI Yuyu, LI Hongyu, DONG Liping, LIU Na. Screening results of 223 368 cases of neonatal genetic metabolic diseases in Zibo by tandem mass spectrometry[J]. Journal of Clinical Medicine in Practice, 2023, 27(11): 64-69. DOI: 10.7619/jcmp.20230648
[5]	PANG Hanqing, SHANG Xiaoyu, WEI Ye, DING Shuwen, LIU Liang. Mechanism of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and network pharmacology[J]. Journal of Clinical Medicine in Practice, 2022, 26(24): 13-19. DOI: 10.7619/jcmp.20223482
[6]	PU Linmei, ZHANG Hongyan, CHEN Xingqiong, CHEN Shifen, NI Yuanyuan, SONG Xian. Concentration of amphotericin B in blood based on two-dimensional high performance liquid chromatography[J]. Journal of Clinical Medicine in Practice, 2022, 26(16): 117-121. DOI: 10.7619/jcmp.20220148
[7]	FENG Linhan, CHENG Qiang, LIU Dinghua, ZHENG Tian, ZHOU Ke, LIU Jiayun. Application of matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry system in identification of pathogenic bacteria[J]. Journal of Clinical Medicine in Practice, 2021, 25(14): 1-3. DOI: 10.7619/jcmp.20210544
[8]	ZHAO Liang, TONG Jian, LI Jianxiang, TAO Jilai. Determination of dihydrocodeine in human plasma by HPLC-MS/MS[J]. Journal of Clinical Medicine in Practice, 2013, (21): 4-7. DOI: 10.7619/jcmp.201321002
[9]	Qiu Xing-an. Determination of cinnamic acid in Mailuoning mixture by HPLC[J]. Journal of Clinical Medicine in Practice, 2011, (11): 118-120. DOI: 10.3969/j.issn.1672-2353.2011.11.042

Cited By

Cited by

Periodical cited type(2)

1.	高振，张晓琳，孟祥宝. 经颅彩色多普勒超声对重度颈动脉狭窄行颈动脉内膜切除术患者前交通动脉开放的作用研究. 中国医疗器械信息. 2023(04): 91-93 .
2.	李丽，朱好辉. TCCD联合颈动脉超声在颈动脉内膜切除术前风险评估中的应用价值研究. 临床研究. 2023(08): 15-17 .

Other cited types(0)

Get Citation

PDF

XML

Article views (243) PDF downloads (13) Cited by(2)

Turn off MathJax

Article Contents

Abstract

References

A study on fingerprint and common peaks identification of water-soluble components in Bombyx Batryticatus