Effects of electroacupuncture on hippocampal neuron autophagy and IGF-1/PI3K/Akt pathway in rats with hypoxic-ischemic brain damage

CAO Juan; WANG Hongyi; WANG Lixin; CHEN Xiaoning; LIN Qiu; YANG Qinghong; CHEN Dongzhui; FU Yuxiu

doi:10.7619/jcmp.20214746

Journal of Clinical Medicine in Practice > 2022 > 26(14): 38-45. > DOI: 10.7619/jcmp.20214746

CAO Juan, WANG Hongyi, WANG Lixin, CHEN Xiaoning, LIN Qiu, YANG Qinghong, CHEN Dongzhui, FU Yuxiu. Effects of electroacupuncture on hippocampal neuron autophagy and IGF-1/PI3K/Akt pathway in rats with hypoxic-ischemic brain damage[J]. Journal of Clinical Medicine in Practice, 2022, 26(14): 38-45. DOI: 10.7619/jcmp.20214746

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Effects of electroacupuncture on hippocampal neuron autophagy and IGF-1/PI3K/Akt pathway in rats with hypoxic-ischemic brain damage

1.
Hainan Modern Women and Children's Hospital, Haikou, Hainan, 571100
2.
Teaching and Research Section of Immunology of College of Basic Medicine and Life Sciences of Hainan Medical University, Haikou, Hainan, 571100

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Received Date: December 01, 2021
Available Online: July 13, 2022

Graphical Abstract

Abstract

Abstract

Objective
To explore the effects of electroacupuncture on hippocampal neuron autophagy and insulin-like growth factor 1 (IGF-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway in neonatal rats with hypoxic-ischemic brain damage (HIBD).
Methods
A total of 108 neonatal rats were randomly divided into sham operation group, model group, IGF-1 group (0.2 mg/kg), electroacupuncture group, electroacupuncture combined with LY294002 group (electroacupuncture combined with 0.3 mg/kg PI3K inhibitor), with 18 rats in each group. HIBD model of neonatal rats was established by ligation of left common carotid artery and hypoxia treatment for 2 hours (the success rate of modeling was 80%). The rats in each group were scored for neurological deficits at being awake after the operation and after electroacupuncture treatment; ELISA was used to detect the content of IGF-1 in brain tissues; hematoxylin-eosin (HE) staining was used to observe the pathological change of brain tissues; transmission electron microscope was used to observe the cell autophagy; immunofluorescence double-labeling method was used to detect the colocalization expression of autophagy markers and neuron-specific nuclear protein (NeuN); western blot method was used to detect the expression of PI3K/Akt pathway and autophagy-related proteins in hippocampus[PI3K, phosphorylated PI3K (p-PI3K), Akt, phosphorylated Akt (p-Akt), Beclin-1, microtubule-related protein light chain 3Ⅱ (LC3-Ⅱ), and microtubule-related protein light chain 3Ⅰ (LC3-Ⅰ)].
Results
Compared with the sham operation group, the neuron damage in the hippocampus of the HIBD model group was aggravated, the neurological deficit score, the Beclin-1 and LC3-Ⅱ/LC3-I expression in the hippocampus were increased, while the IGF-1 content in brain tissues, p-PI3K/PI3K and p-Akt/Akt expression in hippocampus were reduced; compared with the model group, the neuron damage in the IGF-1 group and the electroacupuncture group was alleviated, the neurological deficit score, Beclin-1 and LC3-Ⅱ/LC3-Ⅰ, the number of autophagosomes and the LC3/NeuN co-expressed neurons were decreased or reduced, while the IGF-1 content in brain tissues, p-PI3K/PI3K and p-Akt/Akt expression were increased; the differences mentioned above were statistically significant (P<0.05). LY294002 was able to significantly reduce the level of IGF-1, weaken the activation of the IGF-1/PI3K/Akt pathway of hippocampus and the inhibition of hippocampal neuron autophagy by electroacupuncture.
Conclusion
Electroacupuncture may inhibit excessive autophagy of hippocampal neurons by activating the IGF-1/PI3K/Akt pathway, thereby alleviating HIBD in neonatal rats.
- electroacupuncture,
- hypoxic-ischemic brain damage,
- autophagy,
- insulin-like growth factor 1,
- phosphatidylinositol 3 kinase,
- protein kinase B

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References (27)

References

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Effects of electroacupuncture on hippocampal neuron autophagy and IGF-1/PI3K/Akt pathway in rats with hypoxic-ischemic brain damage