Characteristics of gut microbiota of pregnant women with gestational diabetes mellitus and patients with type 2 diabetes mellitus

QING Yao; ZHOU Huanyu; ZHOU Chaomeng; SONG Zhe; WEI Jinhua; GAO Jianbo

doi:10.7619/jcmp.20221338

Journal of Clinical Medicine in Practice > 2022 > 26(17): 104-110. > DOI: 10.7619/jcmp.20221338

QING Yao, ZHOU Huanyu, ZHOU Chaomeng, SONG Zhe, WEI Jinhua, GAO Jianbo. Characteristics of gut microbiota of pregnant women with gestational diabetes mellitus and patients with type 2 diabetes mellitus[J]. Journal of Clinical Medicine in Practice, 2022, 26(17): 104-110. DOI: 10.7619/jcmp.20221338

Citation:

PDF (2836 KB)

Characteristics of gut microbiota of pregnant women with gestational diabetes mellitus and patients with type 2 diabetes mellitus

1.
Postgraduate Department of Bengbu Medical College, Bengbu, Anhui, 233000
2.
Postgraduate Department of Dalian Medical University, Dalian, Liaoning, 116000
3.
Department of Obstetrics, Changzhou Second People′s Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu, 213000
4.
Department of Endocrinology and Metabolism, Changzhou Second People′s Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu, 213000

More Information

Received Date: April 23, 2022
Available Online: September 20, 2022

Graphical Abstract

Abstract

Abstract

Objective
To investigate the characteristics of gut microbiota in pregnant women with gestational diabetes mellitus (GDM) and type 2 diabetes mellitus (T2DM) patients.
Methods
A total of 61 women were selected as research objects, including GDM pregnant women in group G (n=15), healthy pregnant women in group N (n=18), T2DM patients in group D (n=14), and healthy women in group A (n=14). Four groups of fecal samples were collected and sequenced by 16S rDNA high-throughput sequencing technology. The species diversity of samples and species differences between groups were analyzed.
Results
Intestinal microbiota diversity analysis showed that there were statistically significant differences in Beta diversity between the GDM pregnant women and the healthy pregnant women as well as between the T2DM patients and the healthy women (P < 0.05). In terms of Alpha diversity, the microbiota richness of T2DM patients showed significant difference compared with that of the healthy women (P < 0.05). Compared with the healthy pregnant women, the abundance of Firmicutes in the gut microbiota of the GDM pregnant women was increased, while the abundance of Bacteroidetes and Lachnobacterium were decreased, and the differences were statistically significant (P < 0.05). Compared with the healthy women, the abundance of Phascolarctobacterium in the gut microbiota of T2DM patients was increased, while the abundance of human symbiotic bacteria such as Proteobacteria, Actinobacteria, Roseburia, Turicibacter, Streptococcus, Actinomyces, Coprococcus and Proteus were decreased, and the difference was statistically significant (P < 0.05).
Conclusion
Compared with the healthy women, the gut microbiota of pregnant women with GDM and T2DM patients are significantly different. The results of this study can provide a reference for the future clinical application of intestinal probiotics in the treatment of GDM and T2DM.
- gestational diabetes mellitus,
- type 2 diabetes mellitus,
- gut microbiota,
- 16S rDNA high-throughput sequencing

FullText(HTML)

References (24)

References

[1]	SAEEDI P, PETERSOHN I, SALPEA P, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: results from the International Diabetes Federation Diabetes Atlas, 9th edition[J]. Diabetes Res Clin Pract, 2019, 157: 107843. doi: 10.1016/j.diabres.2019.107843
[2]	ZHU Y, ZHANG C. Prevalence of Gestational Diabetes and Risk of Progression to Type 2 Diabetes: a Global Perspective[J]. Curr Diab Rep 2016, 16(1): 7. doi: 10.1007/s11892-015-0699-x
[3]	DAMM P, HOUSHMAND-OEREGAARD A, KELSTRUP L, et al. Gestational diabetes mellitus and long-term consequences for mother and offspring: a view from Denmark[J]. Diabetologia, 2016, 59(7): 1396-1399. doi: 10.1007/s00125-016-3985-5
[4]	FEIG D S. Type 2 diabetes after gestational diabetes: can the progression be prevented?[J]. Diabetes Metab Res Rev, 2018, 34(4): e2988. doi: 10.1002/dmrr.2988
[5]	HASAIN Z, MOKHTAR N M, KAMARUDDIN N A, et al. Gut microbiota and gestational diabetes mellitus: a review of host-gut microbiota interactions and their therapeutic potential[J]. Front Cell Infect Microbiol, 2020, 10: 188. doi: 10.3389/fcimb.2020.00188
[6]	TILG H, ZMORA N, ADOLPH T E, et al. The intestinal microbiota fuelling metabolic inflammation[J]. Nat Rev Immunol, 2020, 20(1): 40-54. doi: 10.1038/s41577-019-0198-4
[7]	WEI J, QING Y, ZHOU H, et al. 16S rRNA gene amplicon sequencing of gut microbiota in gestational diabetes mellitus and their correlation with disease risk factors[J]. J Endocrinol Invest, 2022, 45(2): 279-289. doi: 10.1007/s40618-021-01595-4
[8]	中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2020年版)(上)[J]. 中国实用内科杂志, 2021, 41(8): 668-695. https://www.cnki.com.cn/Article/CJFDTOTAL-SYNK202108007.htm
[9]	KOREN O, GOODRICH J K, CULLENDER T C, et al. Host remodeling of the gut microbiome and metabolic changes during pregnancy[J]. Cell, 2012, 150(3): 470-480. doi: 10.1016/j.cell.2012.07.008
[10]	PONZO V, FEDELE D, GOITRE I, et al. Diet-gut microbiota interactions and gestational diabetes mellitus (GDM)[J]. Nutrients, 2019, 11(2): E330. doi: 10.3390/nu11020330
[11]	高婉丽. 妊娠期糖尿病孕妇孕晚期肠道菌群的特点分析[D]. 郑州: 郑州大学, 2020.
[12]	FERROCINO I, PONZO V, GAMBINO R, et al. Changes in the gut microbiota composition during pregnancy in patients with gestational diabetes mellitus (GDM)[J]. Sci Rep, 2018, 8(1): 12216. doi: 10.1038/s41598-018-30735-9
[13]	LAKSMANA T, KITTICHOTIRAT W, HUANG Y Y, et al. Metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study[J]. Open Dent J, 2012, 6: 255-261. doi: 10.2174/1874210601206010255
[14]	BACKHED F, DING H, WANG T, et al. The gut microbiota as an environmental factor that regulates fat storage[J]. Proc Natl Acad Sci U S A 2004, 101(44): 15718-15723. doi: 10.1073/pnas.0407076101
[15]	符欢. 人粪便中短链脂肪酸的快速分析方法建立和验证及其临床应用[D]. 长春: 长春工业大学, 2020.
[16]	MEIJNIKMAN A S, GERDES V E, NIEUWDORP M, et al. Evaluating causality of gut microbiota in obesity and diabetes in humans[J]. Endocr Rev, 2018, 39(2): 133-153. doi: 10.1210/er.2017-00192
[17]	QIN J J, LI Y R, CAI Z M, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2012, 490(7418): 55-60. doi: 10.1038/nature11450
[18]	LARSEN N, VOGENSEN F K, VAN DEN BERG F W, et al. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults[J]. PLoS One, 2010, 5(2): e9085. doi: 10.1371/journal.pone.0009085
[19]	SATO J, KANAZAWA A, WATADA H. Type 2 diabetes and bacteremia[J]. Ann Nutr Metab, 2017, 71(Suppl 1): 17-22.
[20]	KARLSSON F H, TREMAROLI V, NOOKAEW I, et al. Gut metagenome in European women with normal, impaired and diabetic glucose control[J]. Nature, 2013, 498(7452): 99-103. doi: 10.1038/nature12198
[21]	WU F F, GUO X F, ZHANG J C, et al. Phascolarctobacterium faecium abundant colonization in human gastrointestinal tract[J]. Exp Ther Med, 2017, 14(4): 3122-3126. doi: 10.3892/etm.2017.4878
[22]	BHANDARKAR N S, MOUATT P, MAJZOUB M E, et al. Coffee pulp, a by-product of coffee production, modulates gut microbiota and improves metabolic syndrome in high-carbohydrate, high-fat diet-fed rats[J]. Pathogens, 2021, 10(11): 1369. doi: 10.3390/pathogens10111369
[23]	OLUWAGBEMIGUN K, O'DONOVAN A N, BERDING K, et al. Long-term dietary intake from infancy to late adolescence is associated with gut microbiota composition in young adulthood[J]. Am J Clin Nutr, 2021, 113(3): 647-656. doi: 10.1093/ajcn/nqaa340
[24]	CRUSELL M K W, HANSEN T H, NIELSEN T, et al. Gestational diabetes is associated with change in the gut microbiota composition in third trimester of pregnancy and postpartum[J]. Microbiome, 2018, 6(1): 89. doi: 10.1186/s40168-018-0472-x

[1]	LIU Kebei. Clinical characteristics and prognosis of children with hyperpyretic convulsion[J]. Journal of Clinical Medicine in Practice, 2019, 23(16): 16-19. DOI: 10.7619/jcmp.201916005
[2]	ZHU Yaodong, ZHANG Mei, LI Ping. Clinical characteristics of varian cancer patients with survival time over 3 years and its influencing factors of prognosis[J]. Journal of Clinical Medicine in Practice, 2019, 23(9): 20-23. DOI: 10.7619/jcmp.201909006
[3]	MEN Lan, LI Changfeng, ZHAO Dongmei. The clinical research of meticulous nursing intervention for patients with chronic ulcerative colitis and its prognosis[J]. Journal of Clinical Medicine in Practice, 2017, (12): 35-38. DOI: 10.7619/jcmp.201712011
[4]	CHEN Juan. Impact of individualized nursing measures on efficacy and prognosis for children with bronchial pneumonia[J]. Journal of Clinical Medicine in Practice, 2017, (4): 149-151,161. DOI: 10.7619/jcmp.201704045
[5]	YU Li. Correlation analysis of clinical nursing pathway and prognosis of patients with myocardial infarction[J]. Journal of Clinical Medicine in Practice, 2017, (4): 11-13. DOI: 10.7619/jcmp.201704004
[6]	HE Yan. Effect of perioperative nursing on psychology and clinical curative efficacy in patients with orthopedic surgery[J]. Journal of Clinical Medicine in Practice, 2017, (2): 86-88. DOI: 10.7619/jcmp.201702027
[7]	CHAI Qiong. Effect of comprehensive nursing measures on the curative efficacy and improvement of prognosis of ICU patients with septic shock[J]. Journal of Clinical Medicine in Practice, 2016, (22): 30-32. DOI: 10.7619/jcmp.201622010
[8]	TANG Yao. Curative effect observation and nursing of endoscopic sinus surgery for chronic sinusitis patients[J]. Journal of Clinical Medicine in Practice, 2015, (18): 111-114. DOI: 10.7619/jcmp.201518035
[9]	ZHANG Chunlin, ZHAO Lin. Clinical application of neoadjuvant therapy in advanced gastric cancer and its effect on prognosis[J]. Journal of Clinical Medicine in Practice, 2013, (21): 46-49. DOI: 10.7619/jcmp.201321013
[10]	SU Yinghao, MA Yunlei, YANG Mei, SU Kuiguo. Curative effect of deproteinised calf serum injection treatment combined with naloxone on pulmonary encephalopathy[J]. Journal of Clinical Medicine in Practice, 2013, (17): 124-126. DOI: 10.7619/jcmp.201317045

Cited By

Cited by

Periodical cited type(8)

1.	范小丽，魏敏，苏曼. 基于快乐理念的干预模式对哮喘患儿心理弹性及生活质量的影响. 中华肺部疾病杂志(电子版). 2021(01): 90-92 .
2.	霍仙娜，张利敏，师玉霞. 郑州市区<8岁儿童呼吸道疾病发病情况及多模式健康教育干预效果. 江苏预防医学. 2020(03): 294-296+299 .
3.	陈小端. 精细化护理对哮喘患儿急性发作期情绪及发作次数的影响. 江苏预防医学. 2020(03): 352-353 .
4.	殷艳林. 过渡期护理对哮喘急性发作期患儿疾病不确定感和生活质量的影响. 河南医学研究. 2020(26): 4962-4964 .
5.	胡银花. 过渡期护理对冠心病出院过渡期患者生存质量及自我管理能力的影响. 临床医药实践. 2019(07): 545-547 .
6.	曹忠英. 过渡期护理对缓解期哮喘患儿自我管理的影响. 护理实践与研究. 2019(24): 112-114 .
7.	李小莉. 舒适护理模式在小儿急性哮喘急诊护理中的应用效果观察. 河南医学研究. 2018(06): 1138-1139 .
8.	李明. 过渡期护理模式在儿童紫癜性肾炎出院护理中的应用. 中国当代医药. 2017(23): 179-181 .

Other cited types(1)

Get Citation

PDF

XML

Article views (170) PDF downloads (5) Cited by(9)

Turn off MathJax

Article Contents

Abstract

References

Characteristics of gut microbiota of pregnant women with gestational diabetes mellitus and patients with type 2 diabetes mellitus