| Citation: |
LI Tuojian, ZHANG Chao, CHEN Zongtao, LI Xinliang. Methylation status of Septin 9 in peripheral blood and its relationship with colorectal cancer[J]. Journal of Clinical Medicine in Practice, 2022, 26(23): 5-8, 13. DOI: 10.7619/jcmp.20222618
|
To detect the methylation status of Septin 9 in the peripheral blood of patients with colorectal cancer (CRC) and analyze the relationship between the methylation of Septin 9 and CRC.
A total of 120 patients with CRC were selected as research objects, and the relationship between the methylation status of Septin 9 in the peripheral blood and the medical materials was analyzed; the serum exosomes isolated from patients with CRC and healthy people were used to treat the CRC cells, and the proliferation and apoptosis of CRC cells were detected.
A total of 1 586 people with health examinations were screened for Septin 9, and 56 of whom were positive for methylation of Septin 9, with a positive rate of 3.5%. Among patients with Septin 9 positive, there were 20 cases (35.7%) with abnormal gastrointestinal status (gastrointestinal inflammation or polyps), while only 5.4% (83/1 530) of patients with Septin 9 negative had abnormal gastrointestinal status, which indicated that the methylation status of Septin 9 in the peripheral blood was closely related to the gastrointestinal status. Among 120 CRC patients, the positive rate of methylation of Septin 9 was 78.4% (73/93); in the comparison of different differentiation degrees, the positive rates of Septin 9 methylation in patients with highly differentiated, moderately differentiated and poorly differentiated CRC were 52.6% (10/19), 79.7% (59/74) and 88.9% (24/27) respectively, and the positive rate of Septin 9 methylation in peripheral blood of patients with poorly differentiated CRC was significantly higher than that of patients with highly differentiated CRC (P=0.006). The sensitivity and specificity of Septin 9 methylation in the peripheral blood were 72.3% and 92.5% respectively. Western blot results showed that the expression of Septin 9 in the exosomes of CRC patients was significantly lower than that in the normal people, while the methylation level of Septin 9 in the exosomes was significantly higher than that in the healthy people (P=0.009, 0.003). The results of this study showed that serum exosomes of CRC patients could significantly promote cell proliferation and inhibit cell apoptosis (P < 0.05 or P < 0.01).
Septin 9 can be used as a marker for early screening of CRC, and the mechanism of Septin 9 may be the proliferation and apoptosis resistance of CRC induced by serum exosomes in patients with CRC.
| [1] |
ZENG H M, ZHENG R S, GUO Y M, et al. Cancer survival in China, 2003-2005: a population-based study[J]. Int J Cancer, 2015, 136(8): 1921-1930. doi: 10.1002/ijc.29227
|
| [2] |
KANG Q, JIN P, YANG L, et al. Significance of Septin 9 gene methylation detection of plasma circulation DNA in colorectal cancer screening[J]. Zhonghua Yi Xue Za Zhi, 2014, 94(48): 3839-3841. .
|
| [3] |
SUN G P, MENG J, DUAN H, et al. Diagnostic ssessment of Septin 9 DNA methylation for colorectal cancer using blood detection: a meta-analysis[J]. Pathol Oncol Res, 2019, 25(4): 1525-1534. doi: 10.1007/s12253-018-0559-5
|
| [4] |
LIU T, ZHANG X, DU L T, et al. Exosome transmitted miR-l 28-3p increase chemosensitivity of oxaliplatin-resistant coloretal cancer[J]. Mol Cancer, 2019, 18(1): 43. doi: 10.1186/s12943-019-0981-7
|
| [5] |
谭琪, 宗明, 處珊珊, 等. 联合检测外周血游离Septin 9、SDC2、BCATI基因甲基化在结直肠癌诊断中的意义[J]. 中华检验医学杂志, 2021, 44(3): 204-211. https://www.cnki.com.cn/Article/CJFDTOTAL-ZMXZ201605028.htm
|
| [6] |
YANQING H, CHENG D, LING X. Serum CA72-4 as a bionarker in the diagnosis of colorectal cancer: a meta-analysis[J]. Open Med: Wars, 2018, 13: 164-171. doi: 10.1515/med-2018-0026
|
| [7] |
宋慧琴, 张君娜. Septin 9基因、缺氧诱导因子1与结直肠癌发生发展的相关性[J]. 广州医科大学学报, 2021, 49(1): 14-17. doi: 10.3969/j.issn.2095-9664.2021.01.04
|
| [8] |
YANG J K, YANG J P, TONG J, et al. Exosomal miR-221 targets DNM3 to induce tumor progression and temozolomide re sistance in glioma[J]. J Neurooncol, 2017, 131(2): 255-265. doi: 10.1007/s11060-016-2308-5
|
| [9] |
陈培, 邓钦木, 李丹丹. 血浆Septin 9甲基化检测在结直肠癌中的诊断价值[J]. 现代检验医学杂志, 2020, 35(4): 10-13. doi: 10.3969/j.issn.1671-7414.2020.04.003
|
| [10] |
宫媛, 王卫华, 杜海涛, 等. 外周血Septin 9基因甲基化检测在结直肠癌诊断中的研究进展[J]. 临床输血与检验, 2021, 23(2): 263-268. doi: 10.3969/j.issn.1671-2587.2021.02.028
|
| [11] |
张春燕, 于正麟, 王蓓丽, 等. 血浆Septin 9基因甲基化检测性能评价及对结直肠癌患者的筛查价值[J]. 临床检验杂志, 2019, 37(2): 152-155. https://www.cnki.com.cn/Article/CJFDTOTAL-LCJY201902018.htm
|
| [12] |
YAN S S, HAN B, GAO S Y, et al. Exsome-encapsulated micro RNAs as circulating biomarkers for colorectal cancer[J] Oncotarget, 2017, 8(36): 60149-60158. . doi: 10.18632/oncotarget.18557
|
| [13] |
陈越亚, 陈卫昌. 外周血SEPT9基因甲基化检测在结直肠癌中的研究进展[J]. 中国血液流变学杂志, 2021, 31(2): 265-270. doi: 10.3969/j.issn.1009-881X.2021.02.031
|
| [14] |
穆剑强, 高海锋. 外周血Septin9基因甲基化和血清CA199联合检测在结直肠癌筛查中的诊断价值[J]. 检验医学与临床, 2022, 19(4): 526-529. https://www.cnki.com.cn/Article/CJFDTOTAL-JYYL202204023.htm
|
| [15] |
RICHARDS K E, ZELENIAK A E, FISHEL M L, et al. Cancer-associated fibroblast exosomes regulate survival and proliferation of pancreatic cancer cells[J]. Oncogene, 2017, 36(13): 1770-1778.
|
| [16] |
赵媛, 陈四明, 蒋益兰, 等. Septin-9基因甲基化检测对结直肠癌诊断价值的meta分析[J]. 中国医师杂志, 2020, 22(6): 852-856. doi: 10.3760/cma.j.cn431274-20200222-00172
|
| 1. |
俞正勇，曹德茂，齐文涛，王小洪，段晓春. 眶上外侧入路治疗急性期破裂大脑中动脉动脉瘤的经验体会. 实用临床医药杂志. 2022(06): 18-22 .
 本站查看
| |
| 2. |
胡海斌. 动脉瘤夹闭术后脑脊液引流与直接缝合硬脑膜对脑积水及脑血管痉挛风险的影响. 中外医学研究. 2019(04): 1-3 .
| |
| 3. |
计跃，裴本根，张健，张玉华，尹玮，夏雪，孙志翔，杨奎，孙克. 腰大池持续引流在动脉瘤性蛛网膜下腔出血血管内栓塞术后的治疗作用. 安徽医学. 2018(10): 1259-1261 .
| |
| 4. |
卢国旺. 腰大池持续脑脊液引流术治疗颅脑损伤术后脑脊液漏的临床研究. 中国医学工程. 2017(08): 70-72 .
|
© 2020 《实用临床医药杂志》编辑部
Address: 江苏省扬州市江阳中路136号，扬州大学江阳路北校区14号楼201室China Pos: 225009Tel: 0514-87978917、87978989、87978807
Supported by:
Beijing Renhe Information Technology Co., Ltd.
苏公网安备 32100302010246号
DownLoad: