Correlations between expressions of SEPT9 gene methylation, growth differentiation factor 15, carbohydrate antigen 199 and clinicopathologic features, prognosis in patients with colorectal cancer

  Objective  To investigate the expressions of SEPT9 gene methylation (mSEPT9), growth differentiation factor 15 (GDF15) and carbohydrate antigen 199 (CA199) in patients with colorectal cancer, and to analyze their relationships with clinicopathological features and prognosis.

  Methods  Blood samples from 218 inpatients admitted to the Anorectal Department were retrospectively analyzed, including 106 patients with colorectal cancer in cancer group and 112 patients with colorectal adenoma in adenoma group. A total of 100 healthy people were collected as control group. The positive rates of mSEPT9, GDF15 and CA199 in the three groups were compared, and the relationships between mSEPT9, GDF15, CA199 and clinicopathological features, prognosis of colorectal cancer patients were investigated.

  Results  The positive rate of mSEPT9, levels ofGDF15 and CA199 in the cancer group were significantly higher than those in the adenoma group and the control group, and were higher in the adenoma group than those in the control group (P < 0.05). The levels of mSEPT9, GDF15 and CA199TNM in patients in stage Ⅲ to Ⅳ, infiltration depth below serosal layer, low differentiation, lymph node metastasis and distant metastasis were significantly increased (P < 0.05). Pearson correlation analysis showed that mSEPT9, GDF15, CA199 were significantly correlated with TNM stage, infiltration depth, differentiation degree, lymph node metastasis and distant metastasis (P < 0.05). High clinical stage, low degree of differentiation, lymph node metastasis, distant metastasis and positive expressions of mSEPT9, GDF15 and CA199 were independent risk factors for poor prognosis in colorectal cancer patients (P < 0.05).

  Conclusion  The expressions of mSEPT9, GDF15, and CA199 in colorectal cancer patients are significantly increased, and are closely related to different clinicopathological features. Positive expressions of mSEPT9, GDF15, and CA199 are independent risk factors for poor prognosis. Clinical intervention should be strengthened to improve the prognosis and survival rate of patients.