Relationships of circulating tumor cells with prognosis and tumor status of triple-negative breast cancer

WANG Hao; LI Nanlin; CAO Hailing

doi:10.7619/jcmp.20230815

Journal of Clinical Medicine in Practice > 2023 > 27(18): 39-43. > DOI: 10.7619/jcmp.20230815

WANG Hao, LI Nanlin, CAO Hailing. Relationships of circulating tumor cells with prognosis and tumor status of triple-negative breast cancer[J]. Journal of Clinical Medicine in Practice, 2023, 27(18): 39-43. DOI: 10.7619/jcmp.20230815

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Relationships of circulating tumor cells with prognosis and tumor status of triple-negative breast cancer

1.
Department of Pathology, Panyu District Central Hospital of Guangdong Province, Guangzhou, Guangdong, 510000
2.
Department of Thyroid Gland and Mammary Gland Surgery, Xijing Hospital of Air Force Military Medical University of Chinese People's Liberation Army, Xi'an, Shaanxi, 710032
3.
Department of Thyroid Gland and Mammary Gland Surgery, Panyu District Central Hospital of Guangdong Province, Guangzhou, Guangdong, 510000

More Information

Received Date: March 14, 2023
Revised Date: August 29, 2023
Available Online: October 08, 2023

Graphical Abstract

Abstract

Abstract

Objective
To analyze the relationships of circulating tumor cell (CTC) with prognosis and tumor status of triple-negative breast cancer(TNBC).
Methods
CTC was isolated from the peripheral blood of 32 patients confirmed as TNBC and 30 healthy controls, and molecular characteristics were analyzed.
Results
Compared with 23 patients with CTC count < 5 in 3.2 mL blood samples, 8 patients with CTC count≥5 had worse progression-free survival and overall survival, and the difference was statistically significant (P=0.033, 0.006), suggesting that the CTC count was valuable in evaluating the prognosis of TNBC patients; among patients with CTC count ≥5 in 3.2 mL blood samples, the presence of CTC clusters was associated with poorer overall survival (P=0.001). Epithelial, mesenchymal, and dry marker gene expression of CTC showed important cellular plasticity, suggesting that the intermediate phenotype of epithelial mesenchymal transformation was associated with effective tumor metastasis, and the molecular characteristics of CTC can effectively predict patients' prognosis.
Conclusion
The molecular features of CTC can reveal the tumor status of patients with advanced TNBC and can be used as highly specific biomarkers with the potential for noninvasive prognostic monitoring.
- circulation tumor cells,
- triple-negative breast tumor,
- prognosis,
- metastasis,
- epithelial mesenchymal transformation

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References (19)

References

[1]	林小龙, 黄心瑜, 周一智. 乳腺癌组织环状RNA ANKS1B及上游转录因子1的表达及临床意义[J]. 实用临床医药杂志, 2023, 27(5): 55-60. doi: 10.7619/jcmp.20222989
[2]	LUO J Y, LAI J G. Pyroptosis-related molecular classification and immune microenvironment infiltration in breast cancer: a novel therapeutic target[J]. J Cellular Molecular Medi, 2022, 26(8): 2259-2272. doi: 10.1111/jcmm.17247
[3]	MANI S, HANDE A, BOICHUK S. Triple-negative breast cancer: the current aspects of pathogenesis and therapies[J]. BioNanoScience, 2022, 12(4): 1404-1435. doi: 10.1007/s12668-022-00991-1
[4]	RING A, NGUYEN-STRÄULI B D, WICKI A, et al. Biology, vulnerabilities and clinical applications of circulating tumour cells[J]. Nat Rev Cancer, 2023, 23(2): 95-111. doi: 10.1038/s41568-022-00536-4
[5]	ADDANKI S, MEAS S, SARLI V N, et al. Applications of circulating tumor cells and circulating tumor DNA in precision oncology for breast cancers[J]. Int J Mol Sci, 2022, 23(14): 7843. doi: 10.3390/ijms23147843
[6]	LISENCU L A, TRANCǍ S, BONCI E A, et al. The role of circulating tumor cells in the prognosis of metastatic triple-negative breast cancers: a systematic review of the literature[J]. Biomedicines, 2022, 10(4): 769. doi: 10.3390/biomedicines10040769
[7]	AGELAKI S, DRAGOLIA M, MARKONANOLAKI H, et al. Phenotypic characterization of circulating tumor cells in triple negative breast cancer patients[J]. Oncotarget, 2017, 8(3): 5309-5322. doi: 10.18632/oncotarget.14144
[8]	ORRAPIN S, UDOMRUK S, LAPISATEPUN W, et al. Clinical implication of circulating tumor cells expressing epithelial mesenchymal transition (EMT) and cancer stem cell (CSC) markers and their perspective in HCC: a systematic review[J]. Cancers, 2022, 14(14): 3373. doi: 10.3390/cancers14143373
[9]	王会珂, 罗茜, 魏千, 等. 基于炎症-免疫-营养评分和循环肿瘤细胞对食管癌根治术后患者构建预后列线图[J]. 中华肿瘤防治杂志, 2023, 30(4): 225-238. https://www.cnki.com.cn/Article/CJFDTOTAL-QLZL202304007.htm
[10]	马新寓, 郑亚赏, 周雪冰, 等. 循环肿瘤细胞在乳腺癌诊疗中的应用[J]. 生命科学, 2022, 34(4): 477-483. https://www.cnki.com.cn/Article/CJFDTOTAL-SMKX202204013.htm
[11]	俞毅麟, 肖艺, 赵娟娟, 等. 三阴性乳腺癌耐药细胞模型的构建及其生物学特性考察[J]. 中国药学杂志, 2022, 57(22): 1901-1910. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYX202222006.htm
[12]	ZHANG Y W, LV Y D, NIU Y D, et al. Role of circulating tumor cell (CTC) monitoring in evaluating prognosis of triple-negative breast cancer patients in China[J]. Med Sci Monit, 2017, 23: 3071-3079. doi: 10.12659/MSM.902637
[13]	MAGBANUA M J, CAREY L A, DELUCA A, et al. Circulating tumor cell analysis in metastatic triple-negative breast cancers[J]. Clin Cancer Res, 2015, 21(5): 1098-1105. doi: 10.1158/1078-0432.CCR-14-1948
[14]	BARBAZÁN J, VIEITO M, ABALO A, et al. A logistic model for the detection of circulating tumour cells in human metastatic colorectal cancer[J]. J Cell Mol Med, 2012, 16(10): 2342-2349. doi: 10.1111/j.1582-4934.2012.01544.x
[15]	YAMAMOTO A, DOAK A E, CHEUNG K J. Orchestration of collective migration and metastasis by tumor cell clusters[J]. Annu Rev Pathol Mech Dis, 2023, 18: 231-256. doi: 10.1146/annurev-pathmechdis-031521-023557
[16]	KUNDU P, SHANKAR B S. Macrophage induced ERK-TGF-β1 signaling in MCF7 breast cancer cells result in reversible cancer stem cell plasticity and epithelial mesenchymal transition[J]. Biochim Biophys Acta BBA Gen Subj, 2022, 1866(11): 130215. doi: 10.1016/j.bbagen.2022.130215
[17]	SANTOS M, FERREIRA M, OLIVEIRA P, et al. Epithelial-mesenchymal plasticity induced by discontinuous exposure to TGFβ1 promotes tumour growth[J]. Biology, 2022, 11(7): 1046. doi: 10.3390/biology11071046
[18]	FINA E. Signatures of breast cancer progression in the blood: what could be learned from circulating tumor cell transcriptomes[J]. Cancers, 2022, 14(22): 5668. doi: 10.3390/cancers14225668
[19]	PANTEL K, ALIX-PANABIÈRES C. Crucial roles of circulating tumor cells in the metastatic cascade and tumor immune escape: biology and clinical translation[J]. J Immunother Cancer, 2022, 10(12): e005615. doi: 10.1136/jitc-2022-005615

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[2]	WANG Zhongqiao, GAO Yan, SI Fengzhi. Experimental study of microRNA-155-5p regulating epithelial mesenchymal transformation of nasal mucosal epithelial cells by targeting histone deacetylase 1[J]. Journal of Clinical Medicine in Practice, 2022, 26(21): 14-19. DOI: 10.7619/jcmp.20221520
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[7]	LIU Yulei, YANG Dongsheng. Expression levels and significance of EGF,TGF-β1 of skin mesenchymal stem cells in patients with psoriasis[J]. Journal of Clinical Medicine in Practice, 2014, (16): 78-80,84. DOI: 10.7619/jcmp.201416022
[8]	WANG Congren, ZHANG Shou. Experimental research on compound repair of rabbit bone marrow mesenchymal stem cells and sodium hyaluronate to the cartilage[J]. Journal of Clinical Medicine in Practice, 2013, (9): 1-4,8. DOI: 10.7619/jcmp.201309001
[9]	SHAO Wei-bin, XUN Kang, LI Xin, LIU Xiao-xian, XIA Chun-Yin. High glucose induce epithelial-to-mesenchymal transition of rat peritoneal mesothelial cells[J]. Journal of Clinical Medicine in Practice, 2010, (17): 4-7. DOI: 10.3969/j.issn.1672-2353.2010.17.002
[10]	ZHAO Ji-dong, ZHU Jian-zhong, MIAO Zong-lin, QIAN Han-guang, PENG Wei. THE EXPERIMENT STUDY OF THE DIFFERENTIATION OF BONE MARROW MESENCHMAL STEM CELLS TO EXPRESS THE PHENOTYPE OF CHONDROCYTE LINEAGEBY TRANSFORMING GROWTH FACTOR-β3 IN VITRO[J]. Journal of Clinical Medicine in Practice, 2005, (11): 45-48. DOI: 10.3969/j.issn.1672-2353.2005.11.013

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2.	张会单，任冰，邹博，姜文弟. 颈动脉溃疡斑块与缺血性脑卒中发生的关系. 血管与腔内血管外科杂志. 2024(01): 113-116 .
3.	景阳，吕茂昌，付久园，杨植，刘超，曹宏伟. 不同影像检查方法对颈动脉内膜切除术与颈动脉支架植入术后颈动脉狭窄程度的诊断价值. 血管与腔内血管外科杂志. 2024(03): 292-295+331 .
4.	吴国华，张晓飞，王琦，李超，闫雪芳. 老年缺血性脑卒中患者颈动脉斑块特征及血液流变学与疾病分型及严重程度的关系. 实用临床医药杂志. 2024(13): 58-62 . 本站查看
5.	郭小婧. 彩色多普勒超声在冠心病患者颈动脉粥样硬化诊断中的价值. 现代诊断与治疗. 2024(13): 1990-1991+1994 .
6.	王勇. 急性缺血性脑卒中血管狭窄与灌注情况的临床诊断. 医药论坛杂志. 2023(01): 63-66 .
7.	骆玉辉，冯杰雄，杨春梅，张远鸿，冯志仪，冯均良. 头颈部CTA联合颈动脉彩色多普勒超声对老年血管性帕金森综合征的诊断价值. 吉林医学. 2023(06): 1517-1520 .
8.	张燕，於巧，盛尧，康锴. 彩色多普勒超声检测对脑卒中患者颈动脉斑块的诊断价值分析. 婚育与健康. 2023(11): 79-81 .
9.	黄治飞，朱幼玲，董斌，翟登月，徐乔乔. 头颈部CT血管成像评估颈动脉几何形态对颈内动脉起始部粥样斑块的影响. 实用临床医药杂志. 2023(13): 65-70 . 本站查看
10.	邓鹏飞，李递通，陈锦俭. 颈动脉支架植入术后持续性低血压的影响因素. 内科. 2023(03): 219-222 .
11.	李生虎，彭雪英. 颈动脉超声筛查在颈动脉狭窄与脑卒中高危人群中的准确性及灵敏性分析. 现代医用影像学. 2023(08): 1395-1397 .
12.	刘阳梅. 颈动脉超声斑块易损性对冠脉病变严重程度预测价值分析. 影像研究与医学应用. 2023(15): 108-110 .
13.	孙晓鑫，陈静霞，杜文雯，李光雷，郭乃娇. 经颅多普勒超声联合C形臂CT在急性缺血性脑血管介入治疗前的临床预判价值. 实用临床医药杂志. 2023(21): 135-138 . 本站查看
14.	张婷. 颈部血管彩色多普勒超声用于颈动脉狭窄病变中的临床诊断价值分析. 现代诊断与治疗. 2023(14): 2169-2171 .
15.	张振平，李泽群，凌思卓，周富强，刘和时，陈金华. 颈部多普勒超声联合经颅多普勒超声在老年急性脑梗死患者颈动脉狭窄评估中的价值. 山东医药. 2023(34): 77-79 .
16.	张玉树，王为强. 颈动脉斑块及狭窄与缺血性脑卒中患者认知功能障碍的相关性. 中国医学物理学杂志. 2022(02): 236-240 .
17.	吴才标. CVUS对急性缺血性脑卒中患者颈动脉状况的评估价值. 中国老年学杂志. 2022(08): 1810-1813 .
18.	林芳，林经纬. 颈动脉彩色多普勒超声在老年缺血性脑卒中诊断中的应用价值. 深圳中西医结合杂志. 2022(03): 102-104 .
19.	林少鹏，黄小平，丁苇，梁志超，莫才春. 颈部血管彩超诊断老年缺血性脑血管病颈部血管异常及狭窄的价值分析. 现代医用影像学. 2022(04): 749-751+757 .
20.	张宇恒，陈静锋，秦迁，生士凤，宋晓琴，丁素英. 空腹血糖轨迹与新发颈部动脉斑块的相关性. 中华健康管理学杂志. 2022(05): 331-336 .
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22.	张京玲. 经颅多普勒超声联合颈动脉超声诊断在缺血性脑卒中的灵敏性及特异性分析. 世界复合医学. 2022(08): 19-23 .
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24.	吴正欢，史俊，黄红伟. 急性缺血性卒中患者颈动脉溃疡斑块危险因素的探索. 中国卫生标准管理. 2022(23): 95-99 .
25.	刘颖萍，田双艳. 颈动脉超声在老年脑卒中高危人群筛查中的价值探讨. 医药论坛杂志. 2021(04): 132-134 .
26.	高峰，马芳，许继梅，张凤云，杨春健. 超声诊断颈动脉易损斑块形成的影响因素分析. 血管与腔内血管外科杂志. 2021(01): 104-107 .
27.	孙丽坤，赵凤依，常玮鑫. 经颅超声溶栓联合普罗布考治疗颈动脉粥样硬化斑块患者的临床研究. 广西医学. 2021(04): 408-412 .
28.	唐庆武，王青飞，朱相宝. 颈动脉超声检测在脑卒中高危人群筛查中的应用价值研究. 中国当代医药. 2021(13): 162-165 .
29.	罗香云，贺辉，程晶. 彩色多普勒超声对缺血性脑卒中颈动脉斑块性质狭窄程度的评估价值分析. 基层医学论坛. 2021(20): 2905-2907 .
30.	漆丹. 颈部血管彩超对缺血性脑血管病患者颈动脉血管病变的诊断价值. 中国实用医药. 2021(19): 34-36 .
31.	尚绪娟. 颈动脉超声对冠心病合并缺血性脑血管病患者颅内血管狭窄的诊断效能. 当代医学. 2021(24): 55-57 .
32.	苟显娜，赵丽莉，陈黎艳，孙赛男，唐向东. TCD联合CDFI应用对颈内动脉狭窄的诊断价值. 贵州医科大学学报. 2021(08): 949-953 .
33.	刘颖萍，田双艳. 老年脑卒中高危人群筛查中应用颈动脉超声的价值探讨. 医药论坛杂志. 2021(13): 65-67+71 .
34.	黄熙畅，叶思婷，戚芷琪，孟令萃，周婷，周俊合，赵琳，符文彬. 运用三维超声VPQ技术评价针灸治疗颈动脉硬化斑块的临床疗效. 海南医学院学报. 2021(16): 1229-1233 .
35.	唐杨宇. 彩色多普勒超声检查颈动脉IMT及斑块与缺血性脑卒中的相关性分析. 当代医学. 2021(31): 142-143 .
36.	徐耀平. 缺血性脑卒中颈部血管狭窄的超声诊断与磁共振成像的应用对比. 影像研究与医学应用. 2021(20): 185-186 .
37.	陈贝贝. 颈动脉彩色多普勒超声和超声造影微血管成像评估冠状动脉粥样硬化性心脏病患者颈动脉狭窄准确率的对比研究. 实用医技杂志. 2021(12): 1410-1412 .
38.	徐志新，韩为，张利达，张君宇，张国庆，张玲，罗佛赐. 通督调神针刺法对高同型半胱氨酸血症患者颈动脉内-中膜厚度及脑血流动力学的影响. 山西中医药大学学报. 2021(06): 426-430 .
39.	李东林，邱国玲. 心脏彩色多普勒超声诊断小儿先天性心脏病的临床价值研究. 实用临床医药杂志. 2020(03): 13-15 . 本站查看
40.	张海春，叶茂，刘淑军，杨喜红. 彩色多普勒超声诊断脑卒中患者颈动脉斑块的临床价值. 山西医药杂志. 2020(05): 527-529 .
41.	孙丽. 颈动脉超声检查在中老年人体检中的应用分析. 影像研究与医学应用. 2020(05): 166-167 .
42.	赵会霞. 颈部彩超在颈内动脉狭窄及斑块形态评估中应用观察. 医药论坛杂志. 2020(02): 163-165 .
43.	贾晓东，孙飞一，彭湃. 颈动脉超声对颈动脉狭窄及脑卒中高危人群的应用体会. 中国医药指南. 2020(16): 168-169 .
44.	鲍凯. 颈动脉彩色多普勒超声检查在中老年糖尿病中的价值分析. 影像研究与医学应用. 2020(12): 128-129 .
45.	陈维林，陈思铭，欧思敏. 高频彩色多普勒超声诊断缺血性脑卒中患者颈动脉粥样硬化的价值分析. 黑龙江医学. 2020(06): 810-812 .
46.	谢芬，胡杰，王允琴. 老年急性缺血性脑卒中患者血压变异性与颈动脉病变关系及患者预后影响因素分析. 实用心脑肺血管病杂志. 2020(07): 59-64 .
47.	王晓梅，王凤. 彩色多普勒超声下血流速度法与直径法对颈动脉狭窄评估效果对比分析. 社区医学杂志. 2020(11): 811-814 .
48.	江武华. 颈动脉彩超与经颅多普勒联合检查在脑卒中筛查中的应用价值. 中国医学创新. 2020(19): 134-137 .
49.	孟瑶，邓敏，周厚地，伉奕. 西洛他唑与阿司匹林降低2型糖尿病患者颈动脉内膜中层厚度的疗效对比研究. 中国医院用药评价与分析. 2020(07): 773-777 .
50.	吴正晖，张琛. 超声在颈动脉斑块诊断中的临床效果探讨. 基层医学论坛. 2020(22): 3208-3209 .
51.	赵晓燕. 探讨彩色多普勒超声用于中老年患者颈动脉斑块诊断的应用价值. 影像研究与医学应用. 2020(18): 215-217 .
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54.	李均超. 大动脉粥样硬化性脑梗死复发相关因素分析. 黑龙江医学. 2020(09): 1269-1271 .
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57.	杨芳，张佳星，任歆，周卫东. 缺血性脑卒中和非脑卒中病人颈动脉内中膜厚度与狭窄程度的关系. 中西医结合心脑血管病杂志. 2020(19): 3299-3301 .
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59.	赵晓楠. 缺血性脑血管病中颈动脉超声的应用价值研究. 中国医疗器械信息. 2020(20): 162-164 .
60.	党玉梅，金仁波，拜钱，马金梅. 超声造影评估颈动脉斑块稳定性及对脑卒中的预测价值. 医学影像学杂志. 2020(10): 1942-1945 .
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