To explore the value of diffusion weighted imaging (DWI) in presurgical planning of pelvic bone neoplasms.

The clinical, imaging and pathological data of patients pathologically confirmed as pelvic chondrosarcoma and osteosarcoma from December 2014 to October 2021 were retrospectively analyzed. Thirteen patients with osteosarcoma and 22 patients with chondrosarcoma were included in the osteosarcoma group and chondrosarcoma group. Through the registration of CT and corresponding DWI images, the tumor boundary was drawn by the Medraw software to form a three-dimensional pelvis model to design the osteotomy distance. The magnetic resonance imaging (MRI) apparent diffusion coefficient (ADC), postoperative pathological results and short-term recurrence rate of two groups were compared.

Of the 35 patients, 26 involved the ilium and 16 involved the acetabulum, of which 8 involved the ilium alone and 15 involved three or more sites simultaneously. The average ADC value in chondrosarcoma group was (1.21±0.17), which was higher than (0.97±0.21) in the osteosarcoma group (P < 0.000 1). The resection margins of the tumor were negative in all cases, and there was no recurrence within 6 months of clinical follow-up.

DWI is helpful to judge the pelvic malignant bone tumors boundaries, and the presurgical planning of osteotomy range based on DWI and ADC value can help individualized operation design.

To investigate the predictive value of conventional ultrasound combined with ultrasound elastography in axillary lymph node metastasis of early small breast cancer.

A total of 150 female breast cancer patients were selected. According to the histopathological results, the patients were divided into axillary lymph node metastasis group (44 cases) and axillary lymph node non-metastatic group (106 cases). Conventional ultrasound was performed on the breast mass and axillary lymph nodes, and ultrasound elastography was performed on the mass. The correlations between the axillary lymph node metastasis and the ultrasound image of the mass and the elastic features of the mass were analyzed.

Univariate analysis showed that breast mass elasticity score, histopathological grade, axillary lymph node minor diameter, cortical thickness, blood supply type and disappearance of lymphatic hilum were correlated with lymph node metastasis (P < 0.05). Multivariate Logistic regression analysis showed that mass elasticity score (P=0.007) and cortical lymph node thickness (P < 0.001) were independent influencing factors for axillary lymph node metastasis in early small breast cancer.

Conventional ultrasound combined with ultrasound elastography is of great value in predicting axillary lymph node metastasis of early small breast cancer, which is conducive to early diagnosis, early treatment and improvement the prognosis of patients.

To explore the predictive value of shear wave elastography (SWE) quantitative parameters and conventional ultrasound features of primary lesions for axillary lymph node metastasis (ALNM) in breast cancer, and to construct a preoperative nomogram model combined with axillary ultrasound.

A total of 295 breast cancer patients who underwent preoperative SWE and conventional ultrasound were selected. Ultrasonic features of primary lesions and axillary lymph nodes were retrospectively analyzed, and independent risk factors associated with ALNM were screened, based on which a nomogram model was constructed and the predictive value was assessed.

Breast tumor uneven margin, E_max and positive for ALNM by ultrasound diagnosis were independent risk factors for predicting ALNM. On this basis, the area under the curve (AUC) of the nomogram model was 0.842 (95%CI, 0.786 to 0.888), and the prediction efficiency was significantly better than that of a single index (P < 0.05).

The quantitative parameters of SWE and conventional ultrasound characteristics of primary lesions of breast cancer can be used to predict ALNM, and the nomogram model constructed in combination with axillary ultrasound has a good value in predicting ALNM.

To explore the application value of two-dimensional ultrasound and shear wave elastography (SWE) in diagnosing breast cancer-related lymphedema (BCRL) and evaluating the efficacy on rehabilitation.

According to the clinical assessment, a total of 102 patients were divided into normal group, subclinical lymphedema group and clinical lymphedema group. Two-dimensional ultrasoundand SWE were used to detect the skin and subcutaneous tissue of the upper limbs of the patients, and the corresponding thickness and hardness were obtained. The correlations of the thickness and hardness of the skin and subcutaneous tissue of the patients in the edema group(subclinical lymphedema group and clinical lymphedema group) with the International Society of Lymphology(ISL)classification were analyzed. The thickness and hardness of skin and subcutaneous tissue of affected limb and contralateral limb in the three groups were compared. The value of circumferential diameter measurement, two dimensional ultrasound and SWE in evaluating therapeutic effect was compared.

In subclinical lymphedema group, the skin thickness, hardness and subcutaneous tissue hardness ofthe forearm of the affected limb were greater than those of the opposite limb (P < 0.05). The skin and subcutaneous tissue thickness and hardness ofthe forearm and upper arm of the affected limb in the clinical lymphedema group were significantly greater than those of the contralateral limb (P < 0.05); forearm skin thickness (r=0.443, P < 0.001) and forearm skin hardness (r=0.275, P=0.024) in the edema group were significantly positively correlated with ISL stage. The receiver operating characteristic curve showed that the area under the curve of two-dimensional ultrasound and SWE in diagnosis of subclinical BCRL was 0.808 and 0.934, respectively. the differences between the two-dimensional ultrasound and SWE assessment of the subclinical lymphedema group before and after treatment were statistically significant (P < 0.05). There was no significant difference in the assessment results of circumference measurement before and after treatment (P=0.293).

Two-dimensional ultrasound and SWE are both of high value for the diagnosis and assessment of treatment effects of BCRL; in early diagnosis, the diagnostic efficacy of SWE is better than that of two-dimensional ultrasound.

To investigate the value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) combined with diffusion weighted imaging (DWI) in diagnosis of small breast cancer lesions (maximum diameter < 2 cm).

A total of 110 patients with 119 breast lesions (maximum diameter < 2 cm) confirmed by surgical pathology were selected. According to different inclusion time, the subjects were assigned to a training set (62 patients with 67 lesions) and a test set (48 patients with 52 lesions). The imaging parameters of benign and malignant lesions were comparatively analyzed. A radiomics model was established by gradient boosting decision tree (GBDT) based on preoperative DCE-MRI and DWI sequences to predict benign and malignant lesions in the test set. The receiver operating characteristic (ROC) curve was drawn to analyze and compare the diagnostic efficacy of GBDT model and three methods of radiologists.

Flux rate constant (K^ep), transfer constant (K^trans), minimum apparent diffusion coefficient (ADC_min), mean apparent diffusion coefficient (ADC_mean) of benign lesions were higher than those of malignant lesions, while extravascular extracellular volume fraction (Ve) was lower than that of malignant lesions (P < 0.05). The maximum diameter of lesions, detected rates of malignant and benign lesions in the training set were similar to those in the test set (P > 0.05). ROC curve showed that the area under the ROC curve (AUC) of GBDT model in the diagnosis of breast cancer smaller than 2 cm was 0.945, which showed no significant difference compared with 0.923 by combined diagnosis of DCE-MRI and DWI (P > 0.05), and was larger than 0.845, 0.851 by DCE-MRI and DWI alone (P < 0.05). The sensitivity, specificity and accuracy of the best cut-off value of GBDT model were 0.91, 0.94 and 0.93, respectively, which showed no significant difference compared with 0.94, 0.81 and 0.86 of DCE-MRI combined with DWI radiomics by radiologists (P > 0.05).

DCE-MRI combined with DWI radiomics has higher value in the diagnosis of breast cancer lesions smaller than 2 cm when combining with GBDT model, and the diagnostic results are consistent with those from experienced radiologists based on DCE-MRI combined with DWI.

To investigate the diagnostic value of color Doppler ultrasound, ultrasound-guided fine needle aspiration cytology (US-FNAC) combined with BRAF^V600E gene detection in small thyroid nodules.

A total of 103 patients with moderate or high risk small thyroid nodules were selected as study subjects. The Thyroid Imaging Reporting and Data System (TI-RADS) grading, preoperative color doppler ultrasound US-FNAC and silk/threonine specific kinase mutant gene V600E (BRAF^V600E) detection were performed. The sensitivity, specificity and accuracy of each examination alone and their combination were compared according to the pathological results, and the diagnostic performance was analyzed.

The ultrasound examination showed that there were statistically significant differences in aspect ratio, structure, echo, echoes, calcification and marginal growth of nodules of different nature (P < 0.05). Of the 103 subjects, 63 cases (98 nodules with 67 malignant ones) were diagnosed as grade V or VI by US-FNAC examination, accounting for 61.2%. There were 26 patients with grade Ⅲ and Ⅳ (32 nodules with 14 malignant ones), accounting for 25.2%. There were 12 cases of grade Ⅱ (12 nodules in total without malignancy), accounting for 11.7%; there were 2 cases of grade Ⅰ (2 nodules with one malignant nodule), accounting for 1.9%. BRAF^V600E gene examination showed that 72 cases (111 nodules, 76 of which were malignant) was detected, accounting for 69.9%. There were 31 patients without mutation (33 nodules in total, 6 of which were malignant), accounting for 30.1%. The sensitivity, specificity and accuracy of ultrasound examination, US-FNAC examination and BRAF^V600E gene test were 75.3%, 62.5%, 73.0%, respectively, 79.5%, 68.8%, 77.5%, respectively and 93.2%, 68.8%, 88.8%, respectively. The sensitivity, specificity and accuracy of their combined diagnosis were the highest, which were 97.3%, 75.0% and 93.3%, respectively. The sensitivity, specificity and accuracy of their combined diagnosis were higher than those of single detection (P < 0.05).

Combined detection can improve the detection rate of thyroid papillary microcarcinoma, which is conducive to accurate preoperative evaluation of small nodules.

To explore the differential value of magnetic resonance diffusion weighted imaging (MRI-DWI) combined with carbohydrate antigen 125 (CA125) in differential diagnosis of borderline ovarian tumors and epithelial ovarian cancer.

The clinical data of 106 patients with suspected borderline ovarian tumor or epithelial ovarian cancer were analyzed retrospectively. All patients were examined with MRI-DWI, and CA125 level was detected. According to the results of pathological examination, they were divided into borderline ovarian tumors group (59 cases) and epithelial ovarian cancer group (47 cases). MRI-DWI and CA125 levels were compared between the two groups. Receiver operating characteristic (ROC) curve was drawn to analyze the identification efficiency of MRI-DWI and CA125 by MRI-DWI and CA125 alone or their combination for borderline ovarian tumors and epithelial ovarian cancer.

The proportion of DWI high signal in the borderline ovarian tumors group was 54.24%, which was higher than 34.04% in the epithelial ovarian cancer group, and the DWI signal intensity was (142.58±16.71), which was higher than that of (126.35±12.20) in the epithelial ovarian cancer group (P < 0.05). Apparent dispersion coefficient (ADC) at different b values of 150, 500, 800 and 1 000×10^-3 mm²/s in the borderline ovarian tumors group were (2.05±0.39)×10^-3 mm²/s, (1.85±0.35)×10^-3 mm²/s, (1.75±0.42)×10^-3 mm²/s, (1.61±0.32)×10^-3 mm²/s, which were higher than those of (1.24±0.31)×10^-3 mm²/s, (1.03±0.22)×10^-3 mm²/s, (0.92±0.18)×10^-3 mm²/s, (0.86±0.17)×10^-3 mm²/s (P < 0.05). R values at b values of 800 and 1 000 s/mm² were (1.58±0.23), (2.15±0.33) respectively, which were higher than (1.20±0.21), (1.52±0.24) in the epithelial ovarian cancer group (P < 0.05). The CA125 level in the borderline ovarian tumors group was (18.12±3.14) U/mL, which was lower than that of (81.50±12.43) U/mL in the epithelial ovarian cancer group (P < 0.05). The cut-off values of ADC with b values of 150, 500, 800 and 1 000 s/mm² were 1.71×10^-3, 1.42×10^-3, 1.31×10^-3, 1.15×10^-3 mm²/s for borderline ovarian cancer and epithelial ovarian cancer, respectively. The cut-off values of ADC with b values 800 and 1 000 s/mm² were 1.42 and 1.71 for borderline ovarian cancer and epithelial ovarian cancer, respectively. The sensitivity and area under curve (AUC) of MRI-DWI combined with CA125 were 100.00% and 0.907 respectively, which were higher than those of MRI-DWI or CA125 alone.

The proportion of high signal, signal intensity, ADC value at different b values, R value at b values of 800 and 1 000 s/mm² and CA125 level in ovarian borderline tumors are all higher than those in epithelial ovarian cancer, and the value of MRI-DWI combined with CA125 in distinguishing them is ideal.

To analyze the research status, hotspots and frontiers of shared decision making in patients with breast cancer based on visualization software.

The literatures in the field of shared decision making for patients with breast cancer from the establishment to January 2023 in core set database of Web of Science were retrieved, and the metrology analysis was performed by CiteSpace 5.7. R1 software.

A total of 1 283 literatures were retrieved, and 759 literatures were finally included for visual analysis. The number of published literatures showed a fluctuating upward trend; the United States had laid the foundation for this research field; the Europe, the United States and other developed countries and their institutions had close cooperation; the research hotspots focused on aspects of different female groups, patients' needs, different decision making stages and the development of intervention techniques in breast cancer patients with sharing decision making.

In recent years, the sharing decision making of breast cancer patients has received continuous attention, and more researches has been done in developed countries. It is suggested that Chinese clinical researchers should broaden their cooperation fields, focus on relevant research hotspots, and promote the development of shared decision making researches on breast cancer patients in China.

To establish a Nomogram model for prediction of recurrence after breast cancer surgery based on multi-dimensional indicators.

A total of 313 female patients with surgical treatment for unilateral breast cancer from March 2014 to April 2020 in authors' hospital were retrospectively selected as research objects, and they were divided into modeling set with 219 cases and validation set with 94 cases by the random number table method at a ratio of 7 to 3. In the modeling set, the patients were divided into recurrence group and non-recurrence group according to recurrence condition during follow-up after operation. Single factor and multi-factor Logistic regression models were used to analyze the risk factors of postoperative recurrence of breast cancer. Based on the screening results, the R software was used to draw the Nomogram prediction model. The performance of the model was evaluated by the receiver operating characteristic (ROC) curve and deviation test of goodness of fit. The calibration map was verified and drawn by computer-simulated repeated sampling method (Bootstrap); the decision curve was used to evaluate the clinical benefit rate of the model.

Among the 219 case in the modeling set, 63 cases (28.77%) had recurrence of breast cancer after surgery. Irregular tumor margin (OR=1.692, 95%CI, 1.154 to 3.794), high maximum elastic modulus of tissues around 3 mm from focus measured by Shell function and tracing method (Shell3 E_max) (OR=2.869, 95%CI, 1.795 to 5.392), lymph node metastasis (OR=2.071, 95%CI, 1.486 to 4.578), high expression of D-dimer (OR=2.264, 95%CI, 1.574 to 5.307) and high ratio of fibrinogen to albumin (OR=3.089, 95%CI, 2.053 to 6.156) were risk factors for postoperative recurrence of patients with breast cancer (P < 0.05). Nomogram model for risk prediction was established based on the five factors mentioned above, the area under the curve of the ROC curve of the model was 0.872 (95%CI, 0.829 to 0.917), the best cut-off value (threshold probability) was 0.32 (32%), and the corresponding sensitivity and specificity were 0.871 and 0.837 respectively; the test of goodness of fit showed that there was no over-fitting phenomenon in the prediction model (χ²=4.204, P=0.826); the Bootstrap method based on 1 000 sampling verifications found that the average absolute error of the calibration curve was 0.019, indicating that the prediction model had a good consistency. The area under the curve of the ROC curve of the Nomogram model for prediction of validation set was 0.864, the sensitivity was 0.862, and the specificity was 0.815; the correction curve was close to the ideal curve. When the threshold probability value in the decision curve was set to 32%, the clinical benefit rates of the population in the modeling set and the validation set were 56% and 62% respectively.

Nomogram model based on tumor margin, Shell3 E_max value, condition of lymph node metastasis, serum D-dimer and the ratio of fibrinogen to albumin of breast cancer patients has a certain value in predicting the risk of postoperative recurrence.

To explore the medication rules of professor Song Aili in treating granulomatous lobular mastitis (GLM) and the mechanism of core herbal pairs based on data mining and network pharmacology methods.

From June 2021 to June 2022, the traditional Chinese medicine prescriptions taken by GLM patients treated by Professor Song Aili in the outpatient and inpatient departments of the Affiliated Hospital of Shandong University of Traditional Chinese Medicine were collected. Relevant medication rules were explored through the TCM inheritance auxiliary platform, and the mechanism of core herbal pairs was analyzed by network pharmacology.

A total of 248 traditional Chinese medicine prescriptions were included in this study, and the herbal pair of Jinyinhua (lonicerae japonicae flos) with Lianqiao (forsythiae fructus) was the core herbal pair used by professor Song Aili for treatment of this disease; the important active ingredients of the herbal pair of Jinyinhua with Lianqiao were quercetin, luteolin and wogonin; the key targets of the herbal pair of Jinyinhua with Lianqiao on GLM were tumor necrosis factor (TNF), interleukin-6 (IL-6), interleukin-1β (IL-1β), CXC chemokine ligand 8 (CXCL8), chemokine ligand 2 (CCL2) and etc.; the main signal pathways regulated by active ingredients of herbal pair included interleukin-17 (IL-17) signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway and etc.

Professor Song Aili mainly treat GLM from Yang syndrome based on syndrome differentiation. Removal of phlegm and blood stasis, permeation of sepsis and detoxification, and nourishment of qi and blood are regarded as the core therapeutic method, and Jinyinhua with Lianqiao as core herbal pair that may obtain therapeutic effect on GLM by regulating IL-17 signaling pathway, Toll-like receptor signaling pathway and TNF signaling pathway.

To investigate the expression and clinical significance of CircRNA ANKS1B (CircANKS1B) and upstream transcription factor 1 (USF1) in breast cancer tissues.

A total of 90 patients with breast cancer were selected as subjects. USF1 protein expression in tissues was detected by immunohistochemistry. The expression of CircANKS1B and USF1 mRNA in tissues was detected by real-time quantitative polymerase chain reaction. The correlation between CircANKS1B and USF1 mRNA in cancer tissues was analyzed. The relationships of CircANKS1B and USF1 mRNA expression with clinicopathologic features of breast cancer were analyzed. The relationships of CircANKS1B and USF1 mRNA expression with prognosis of breast cancer were analyzed. Univariate and multivariate COX proportional risk models were used to analyze the independent influencing factors of the prognosis of breast cancer.

The brownish-yellow expression of USF1 in breast cancer tissues was mainly located in the nucleus. The positive rate of USF1 protein expression in breast cancer tissues was 86.67% (78/90), which was significantly higher than 15.56% (14/90) in adjacent tissue (P < 0.001). The relative expression levels of CircANKS1B and USF1 mRNA in the breast cancer tissues were (4.12±0.68) and (6.35±1.21), respectively, which were significantly higher than (1.23± 0.46) and (1.57±0.59) in the adjacent tissue (P < 0.001). There was a significant positive correlation between CircANKS1B and USF1 mRNA expression in breast cancer tissues (r=0.604, P < 0.001). The expression of CircANKS1B and USF1 mRNA showed statistically significant differences in different tumor stages, lymph node metastasis and triple-negative breast cancer (P < 0.05). The median survival time of patients with high CircANKS1B expression was 52.62 months (95%CI, 49.44 to 56.35), which was significantly shorter than 56.78 months (95%CI: 52.46 to 57.05) in patients with low CircANKS1B expression (P=0.045). The median survival time of patients with low expression of USF1 mRNA was 52.53 months (95%CI, 48.83 to 56.23), which was significantly shorter than 56.75 months (95%CI, 54.07 to 59.43) of patients with low USF1 mRNA expression (P=0.014). CircANKS1B high expression, USF1 mRNA high expression, tumor stage Ⅲ and lymph node metastasis are independent influencing factors of the prognosis of breast cancer.

The expressions of CircANKS1B and USF1 are increased in breast cancer tissues, and correlated with TNM stage, lymph node metastasis, and are new breast cancer prognostic tumor markers.

To investigate the effects and mechanisms of LncRNA XIST on proliferation, migration, invasion and apoptosis of breast cancer cells.

Normal mammary epithelial cells MCF-10A and breast cancer cell lines MCF-7, T47D and Bcap-37 cells were selected and transfected into MCF-7 and T47D cells with different substances. They were set as blank group (no transfection), si-XIST group (transfection si-XIST), si-NC group (transfection si-NC), microRNA-20a-5p group (transfection miR-20a-5p mimics) and miR-NC group (transfection miR-NC). CCK-8 was used to detect cell proliferation; Transwell was used to detect cell invasion ability; cell migration ability was detected by scratch test; apoptosis was detected by TUNEL staining. Luciferase reporter gene assay was used to verify the targeting relationships between LncRNA XIST and miR-20a-5p as well as between miR-20a-5p and high mobility group protein A2 (HMGA2). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of LncRNA XIST and miR-20a-5p. The expression level of HMGA2 was detected by Western blot.

Compared with MCF-10A cells, LncRNA XIST expression levels in MCF-7, T47D and Bcap-37 cells were significantly increased, while miR-20a-5p expression level was significantly decreased (P < 0.05). Down-regulating LncRNA XIST or up-regulating miR-20a-5p inhibited proliferation, invasion and migration of breast cancer cells, and promoted cell apoptosis (P < 0.05). LncRNA XIST negatively regulated miR-20a-5p, and miR-20a-5p negatively regulated HMGA2.

Down-regulated LncRNA XIST inhibits proliferation, migration andinvasion of breast cancer cells and promotes cell apoptosis through miR-20a-5p/HMGA2 axis.

To investigate the relationships between the expression of peripheral blood annexin A3 (ANXA3), hypoxia-inducible factor-1α (HIF-1α) and platinum resistance in colorectal cancer.

Clinical data of 122 patients with colorectal cancer treated with platinum-based chemotherapy were retrospectively analyzed. Drug resistance was evaluated after 6 cycles of chemotherapy, and patients were divided into drug resistant group (48 cases) and sensitive group (74 cases) according to their sensitivity to platinum drugs. the clinical data and the levels of ANXA3 and HIF-1α in peripheral blood of the two groups were compared; Logistic regression model was used to analyze the influencing factors of platinum resistance; the receiver operating characteristic (ROC) curve was drawn to analyze the predictive efficacy of ANXA3 and HIF-1α in peripheral blood on platinum-based drug resistance.

There were no significant differences in sex composition, age, TNM stage and histological grade between the drug resistant group and sensitive group (P > 0.05). ANXA3 and HIF-1α levels in peripheral blood of the drug resistant group were significantly higher than those in the sensitive group (P < 0.05). The median progression free survival (PFS) of patients in the drug resistant group was 20.5 months (95%CI, 16.744 to 30.254), and the median PFS of patients in the sensitive group was 30.4 months (95%CI, 23.617 to 34.569). The median PFS of the drug resistant group was significantly shorter than that of the sensitive group (P < 0.05). ROC curve showed that the area under the curve (AUC) for predicting platinum resistance was 0.847 when 2.03 mg/L was the cut-off value; when HIF-1α was truncated at 189.57 pg/mL, the predicted AUC of platinum resistance was 0.722. The AUC of platinum resistance predicted by ANXA3 was significantly larger than that of HIF-1α(Z=2.201, P < 0.05). The AUC of ANXA3 combined with HIF-1α was 0.915, which was significantly larger than that of ANXA3 and HIF-1α alone (Z=2.150, 3.021, P < 0.05). Compared with ANXA3, ANXA3 combined with HIF-1α showed higher sensitivity in predicting platinum resistance; compared with HIF-1α, ANXA3 combined with HIF-1α had higher specificity in predicting platinum resistance.

High levels of ANXA3 and HIF-1α in peripheral blood are closely related to platinum-resistance in colorectal cancer. The combination of ANXA3 and HIF-1α shows high predictive efficacy in the determination of platinum-type drug resistance in colorectal cancer.

To explore the expression and diagnostic value of long non-coding RNA (LncRNA) X-inactive specific transcript (XIST) and microRNA-146-5p (miR-146-5p) in thyroid cancer tissue.

A total of 92 patients with thyroid cancer were selected as research objects. All patients underwent surgical resection, and thyroid cancer tissues and adjacent normal tissues were collected during the operation. The relationships between the levels of LncRNA XIST, miR-146-5p and the clinical pathological characteristics of patients were analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of LncRNA XIST and miR-146-5p alone and their combination in thyroid cancer.

The expression level of LncRNA XIST in the thyroid cancer tissue was (0.65±0.17), which was significantly lower than (1.02±0.18) in the adjacent tissues, and the expression level of miR-146-5p was (2.56±0.87), which was significantly higher than (1.07±0.25) in the adjacent tissues (P < 0.05). The expression levels of LncRNA XIST in thyroid cancer patients with clinical stage Ⅲ to Ⅳ and cervical lymph node metastasis were significantly lower than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis, and the expression level of miR-146-5p was significantly higher than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis (P < 0.05). ROC curve showed that the area under curve (AUC) of LncRNA XIST combined with miR-146-5p in was significantly higher than that of LncRNA XIST and miR-146-5p alone (P < 0.05).

The expression of LncRNA XIST is down-regulated and miR-146-5p is up-regulated in thyroid cancer. Both of them are related to clinical stage and cervical lymph node metastasis, and have certain diagnostic value for thyroid cancer.

To explore the expression level of microRNA-150-5p (miR-150-5p) in nasopharyngeal carcinoma tissue and its effects on proliferation of cancer cells and radiosensitivity.

Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of miR-150-5p in nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cells. Nasopharyngeal carcinoma cells CNE2 were routinely cultured and transfected with miR-150-5p mimic, and were divided into control group (NC group) and miR-150-5p overexpression group (miR-150-5p mimic group). The proliferation ability of CNE2 cells in each group was detected by MTT assay; the apoptosis of CNE2 cells in each group was detected by flow cytometry; the Western blot was used to detect the expressions of phosphorylated phosphatidylinositol 3-kinase (pPI3K), phosphorylated protein kinase B (pAKT) and phosphorylated mammalian target of rapamycin (pmTOR) protein in cells in each group.

The expression level of miR-150-5p in nasopharyngeal carcinoma tissue was (0.74±0.39), which was significantly lower than (1.44±0.54) in paracancerous tissue (t=8.140, P < 0.001). After transfection with 48 hours, the expression level of miR-150-5p in CNE2 cells in the miR-150-5p mimic group was (6.31±1.20), which was significantly higher than (1.00±0.08)in the NC group (t=7.647, P < 0.001). The result of MTT experiment showed that the proliferation ability of CNE2 cells at 24, 48 and 72 hours in the miR-150-5p mimic group was significantly lower than that in the NC group (P < 0.05). After radiation with 0.5, 1.0, 2.0, 4.0, 8.0 and 16.0 Gy, the cell proliferation rates of the miR-150-5p mimic group were significantly lower than those of the NC group (P < 0.05). After radiation of CNE2 cells with 2 Gy, the apoptosis rate of cells in the miR-150-5p mimic group increased significantly when compared to that in the NC group, while the expression levels of pPI3K, pAKT and pmTOR protein in CNE2 cells decreased significantly when compared to that in the NC group (P < 0.05).

The expression of miR-150-5p in nasopharyngeal carcinoma cells is down-regulated. Overexpression of miR-150-5p can inhibit the proliferation of nasopharyngeal carcinoma cells, promote cell apoptosis, and effectively enhance the radiosensitivity of nasopharyngeal carcinoma cells; its role may be played by regulating the PI3K/AKT/mTOR signal pathway, and miR-150-5p may be the target of drugs to enhance the radiosensitivity of nasopharyngeal carcinoma.

To observe the clinical effect of preservation of the left colonic artery (LCA)and superior rectal artery(SRA) in laparoscopic radical resection of sigmoid cancer in elderly patients.

The clinical data of 320 patients with sigmoid colon carcinoma undergoing laparoscopic radical resection were retrospectively analyzed. A total of 129 patients who retained LCA and SRA were included in experimental group, and 191 patients who did not retain LCA and SRA were included in control group. The intraoperative and postoperative conditions of the two groups were compared.

The experimental group underwent no prophylactic ileostomy, and 9 cases in the control group received prophylactic ileostomy, a significant difference was found in two groups(P < 0.05). Operation time was significantly longer in the experimental group than that in the control group (P < 0.05). No significant differences in terms of total number of lymph node dissection and the number of lymph node(No. 253) dissection, intraoperative blood loss, postoperative anastomotic bleeding, incidence of anastomotic leakage, recovery time of bowel sound, postoperative exhaust time and length of hospital stay were found between the two groups(P > 0.05).

Preservation of LCA and SRA in laparoscopic radical resection of sigmoid cancer in elderly patients can reduce preventive terminal ileum stoma and it is safe and feasible in technology.

To compare the effect of canagliflozin (CGLZ) and soy isoflavone (SIF) on bone metabolism in model rats with type 1 diabetes mellitus.

Forty male SD rats were randomly divided into control group, model group, SIF group, low-dose CGLZ group and high-dose CGLZ group, with 8 rats in each group. Model group and treatment groups were intraperitoneally injected with streptozotocin for modeling. The rats in treatment groups were gavaged daily for 12 weeks. During and after treatment, the body mass, food intake, water consumption, fasting blood glucose (FPG), serum albumin (ALB), serum calcium (SCa), serum phosphorus (SP), serum creatinine (Scr), serum 25 hydroxyvitamin D (25-OH-D₃), urine protein (Pro), urine creatinine (Ucr), urine protein to urine creatinine ratio (UPCR) and bone metabolism related indicators[total bone mineral density (TBMD), regional bone mineral density (RBMD), total body salt content (TBSC), total body fat mass (TBFM) and total body muscle mass (TBMM)]of rats in each group were detected. Detection sites of RBMD included head, upper limb, thigh, trunk, rib, pelvis and spine.

After 12 weeks of successful modeling, the water intake and food consumption of rats in model group, SIF group, low-dose CGLZ group and high-dose CGLZ group all increased, while the body mass decreased; after medication, the body mass of rats in low-dose CGLZ group and high-dose CGLZ group increased, and increased range of body mass in high-dose CGLZ group was significantly greater than that in the SIF group. The FPG level of rats after 4 weeks of treatment in low-dose CGLZ group and high-dose CGLZ group as well as after 8 and 12 weeks of treatment in the SIF group, low-dose CGLZ group and high-dose CGLZ group decreased, and efficacy ranking from high to low was high-dose CGLZ group, low-dose CGLZ group and SIF group. After 12 weeks of treatment, Pro, Ucr and UPCR of rats in each treatment group decreased significantly (P < 0.01); in the SIF group, low-dose CGLZ group and high-dose CGLZ group, serum ALB level increased significantly while Scr level decreased significantly(P < 0.01); the level of 25-OH-D₃ in high-dose CGLZ group decreased significantly (P < 0.01). After treatment of 12 weeks, TBSC in high-dose CGLZ group increased, but TBMD, TBSC, TBFM and TBMM did not increase in SIF group and low-dose CGLZ group; the increase of bone mineral density was observed in the thigh of high-dose CGLZ group, the trunk, ribs and spine of low-dose CGLZ group and high-dose CGLZ group, and the pelvis of SIF group and high-dose CGLZ group.

Both CGLZ and SIF can reduce hyperglycemia induced by streptozotocin in model rats with type 1 diabetes mellitus, and the effect of the former is better. In terms of improving the total body bone salts, total body bone mineral density and bone mineral density at all sites, the effect of high-dose CGLZ is better than that of low-dose CGLZ, while SIF can only improve the bone mineral density of pelvis to a certain extent.

To investigate the correlations of transforming growth factor-β (TGF-β)and meteorin-like protein(Metrnl) with macroangiopathy in type 2 diabetes mellitus.

A total of 106 patients with T2DM were divided into simple T2DM group (n=50, CIMT ≤ 1.0 mm), T2DM combined with macroangiopathy disease group (n=56, CIMT>1.0 mm), and 52 healthy subjects who underwent physical examination at the same time were randomly selected as healthy control group. Metrnl and TGF-β levels in peripheral serum in three groups were compared. The correlations of metabolic indexes with T2DM macroangiopathy were compared between the three groups.

The levels of TGF-β and Metrnl in the T2DM combined with macroangiopathy disease group were significantly lower than those in the T2DM simple group and healthy control group (P < 0.05); there was a positive correlation between TGF-β and Metrnl level (P < 0.05); systolic blood pressure, smoking, low-density lipoprotein cholesterol (LDL-C) and HOMA-IR were risk factors for T2DM macroangiopaopathy, while Metrnl and TGF-β were protective factors for T2DM macroangiopaopathy (P < 0.05); receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) predicted by TGF-β and Metrnl were 0.757 and 0.846, respectively, the AUC predicted by TGF-β combined with Metrnl was 0.901.

Serum TGF-β and decreased Metrnl levels are associated with the occurrence of T2DM macrovascular disease. Detection of TGF-β combined with Metrnl is of great significance for the early evaluation of T2DM macrovascular disease.

To explore the role of neurotrophin-3 overexpression induced pluripotent stem cells (iPSC-NT-3) in treating rats with diabetes-induced erectile dysfunction (DIED).

The diabetic model rats were established by intraperitoneal injection of streptozotocin, and the DIED model was verified by subcutaneous injection of apomorphine. Twenty-four rats with successful modeling of DIED were randomly divided into model control group, induced pluripotent stem cell (iPSC) treatment group and IPSC-NT-3 treatment group; eight SD rats with intraperitoneal injection of sodium citrate and citrate buffer were selected as normal control group. Rats in each group were intraperitoneally injected with pentobarbital sodium for anesthesia, the iPSC and the iPSC-NT-3 were respectively injected into the penile sponge of rats through microinjection needle in the iPSC treatment group and the IPSC-NT-3 treatment group, and the equal volume of phosphoric acid buffer was injected by the same method in the normal control group and the model control group. At the 4th week after treatment, the general condition of the rats in each group was observed, and the sexual function and erectile function of the penis were evaluated. Quantitative polymerase chain reaction (q-PCR) and Western blot assay were used to detect the mRNA and protein expressions of neurotrophin-3 (NT-3), CD31, vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), Desmin and α-smooth muscle actin (α-SMA).

Before and 4 weeks after treatment, there were no significant intra-group and between-group differences in body mass and blood glucose level of rats among the model control group, the iPSC treatment group and the iPSC-NT-3 treatment group (P>0.05). In the comparison of primary time of climbing back in rats, the time in the model control group was longer than the normal control group, the time in the iPSC treatment group and the iPSC-NT-3 treatment group was shorter than the model control group, and the time in the iPSC-NT-3 treatment group was shorter than the iPSC treatment group, and all the differences were statistically significant (P < 0.05); in the comparison of licking and smelling times, riding times and inserting times, the times in the model control group were less than the normal control group, the times in the iPSC treatment group and the iPSC-NT-3 treatment group were greater than the model control group, the times in the iPSC-NT-3 treatment group was greater than the iPSC treatment group, and all the differences were statistically significant (P < 0.05). In comparison of ICP and ICP/MAP, the values in the model control group were lower than the normal control group, the values in the iPSC treatment group and the IPSC-NT-3 treatment group were higher than the model control group, and the values in the IPSC-NT-3 treatment group were higher than the iPSC treatment group, and all the differences were statistically significant (P < 0.05). In the comparison of the mRNA expression levels of NT-3, CD31, VEGF, eNOS, Desmin and α-SMA in the cavernous penis of rats, the levels in the model control group were lower than the normal control group, the levels in the iPSC treatment group and the iPSC-NT-3 treatment group were higher than the model control group, the levels in the iPSC-NT-3 treatment group were higher than the iPSC treatment group, and all the differences were statistically significant (P < 0.05). In the comparison of the protein levels of NT-3, CD31, VEGF, eNOS, Desmin and α-SMA in the cavernous penis of rats, the levels in the model control group were lower than the normal control group, the levels in the iPSC treatment group and the IPSC-NT-3 treatment group were higher than the model control group, the levels in the IPSC-NT-3 treatment group were higher than the iPSC treatment group, and all the differences was statistically significant (P < 0.05).

The iPSC-NT-3 can improve the sexual function and erectile function of DIED rats by promoting the production of eNOS, vascular endothelium and smooth muscle in the cavernous tissues, which is a potential treatment for DIED.

To explore the correlation between blood glucose level on admission and prognosis in patients with sepsis.

A total of 290 hospitalized patients with sepsis in the Emergency Department of Affiliated Hospital of Nantong University from April 2016 to October 2021 were selected, and they were divided into normal blood glucose group (blood glucose level ranged from 4.0 to 7.7 mmol/L) with 116 cases, mild hyperglycemia group (blood glucose level ranged from greater than 7.7 to 11.0 mmol/L) with 95 cases, and severe hyperglycemia group (blood glucose level was greater than 11.0 mmol/L) with 79 cases according to the blood glucose level on admission, and hypoglycemia group was excluded. Restricted cubic spline curve was used to analyze the relationship between blood glucose level and 28-day mortality; the Logistic regression model was used to analyze the correlation between blood glucose grade and 28-day mortality; subgroup analysis was performed based on the presence or absence of previous hypertension and diabetes.

Of the 290 patients, 70 cases (24.1%) died within 28 days. Compared with the survival group (n=220), the age, the Sequential Organ Failure Score (SOFA) and the Acute Physiology and Chronic Health Evaluation(APACHE Ⅱ) score of patients in the death group were significantly higher (P < 0.05). In the comparison of baseline characteristic materials between normal blood glucose group, mild hyperglycemia group and severe hyperglycemia group, the results showed that with the aggravation of hyperglycemia severity, the previous prevalence rates of hypertension and diabetes were significantly higher (P=0.01, P < 0.01), while the prothrombin time (PT) and activated partial thrombin time (APTT) significantly shortened (P < 0.01); the 28-day mortalities of the three groups were 18.1%, 22.1% and 35.4% respectively, and there was significant difference among three groups (P=0.02). With the increase of blood glucose level, the risk of 28-day mortality increased in a linear relationship (non-linear test P=0.856); the univariate Logistic regression analysis showed that increases of age, SOFA score and APACHE Ⅱ score as well as increased degree of blood glucose level were correlated with 28-day mortality (P < 0.05); in both model 1 and model 2 established by multivariate regression analysis, severe hyperglycemia was independently associated with 28-day mortality (OR=2.48, 3.38; 95%CI, 1.25 to 4.95, 1.50 to 7.63); the subgroup analysis for the presence or absence of previous hypertension and diabetes showed no significant interaction (P>0.05).

Severe hyperglycemia on hospital admission in patients with sepsis is associated with increased risk of 28-day mortality, which should be considered seriously by clinicians.

To explore the serum levels of vitamin D and ferritin (SF) in diabetic peripheral neuropathy (DPN) and their correlations with oxidative stress.

A total of 216 diabetic patients were included in this study and divided into control group (n=171, no concurrent DPN) and observation group (n=45, concurrent DPN) according to whether the patients were combined with DPN. The general data and serum levels of the two groups were compared. Pearson method and multiple stepwise regression analysis were used to investigate the correlations between serum ferritin, vitamin D and oxidative stress.

SF and malondialdehyde (MDA) levels in the observation group were (575.65±38.42) ng/mL and (18.68±2.15) mmol/L, which were higher than (441.96±51.37) ng/mL and (12.63±3.32) mmol/L, respectively in the control group (P < 0.05). The levels of 25-hydroxyvitamin D[25-(OH)D], reduced glutathione (GSH) and superoxide dismutase (SOD) in the observation group were (10.68±2.25) ng/mL, (104.13±22.15) mg/L and (70.63±11.45) mg/L, respectively, which were lower than (17.47±5.61) ng/mL, (197.58±43.19) mg/L and (114.39±25.87) mg/L, respectively in the control group, and the differences were statistically significant (P < 0.05). Binary Logistic regression analysis showed that SF, 25-(OH)D, GSH, SOD and MDA had certain impacts on DPN complications in patients. Pearson method showed that SF was negatively correlated with GSH, but positively correlated with MDA (P < 0.05). 25-(OH)D was positively correlated with GSH and SOD (P < 0.05). Multiple stepwise regression analysis showed that MDA had a significant positive impact on SF, while GSH had a negative impact on SF (P < 0.05); GSH and SOD had a significant positive impact on 25-(OH)D (P < 0.05).

Oxidative stress, SF and vitamin D are involved in the occurrence and development of DPN, and oxidative stress is closely related to SF and vitamin D.

To analyze levels of microRNA-34a(miR-34a)and hypoxic inducible factor-1α (HIF-1α)in patients with type 2 diabetes(T2DM) with different urinary albumin excretion rates (AER) and their significance.

A total of 90 patients with T2DM were selected as T2DM group. At the same time, 90 healthy adults who underwent physical examinations in outpatient department during the same period were included in control group. According to different urinary AER, the patients in the T2DM group were divided into normal group, micro-amount group and large amount group, with 30 cases in each group. Serum HIF-1α level was detected by double antibody sandwich Enzyme Linked Immunosorbent Assay and the expression level of miR-34a in serum was detected by real-time fluorescence quantitative polymerase chain reaction. Blood glucose, serum HIF-1α, serum miR-34a level and AER level of each group were compared, and the correlations of AER level with serum miR-34a level and serum HIF-1α level in T2DM patients were analyzed, and the relationship between miR-34a and HIF-1α was analyzed. Receiver operating characteristic (ROC) curve was plotted, and the area under the curve (AUC) was calculated to evaluate the predictive value of HIF-1α and miR-34a for T2DM.

Compared with the healthy group, the urinary albumin levels of the normal group, the micro-amount group and the large amount group were significantly higher, and their levels in the micro-amount group were higher than those of the normal group, and were higher in the large amount group than the micro-amount group(P < 0.05). Correlation analysis showed that AER level in T2DM patients was positively correlated with serum HIF-1α level and serum miR-34a level (r=0.75, 0.73, P < 0.05), and serum HIF-1α level was positively correlated with serum miR-34a level (r=0.55, P < 0.05). ROC curve showed that HIF-1α and miR-34a had high predictive value for T2DM, with AUC values of 0.991 and 0.934, respectively.

The higher the urinary albumin level in patients with T2D is, the more serious the disease will be. Serum HIF-1α level is positively correlated with serum miR-34a level, suggesting that miR-34a and HIF-1α are the influencing factors of T2DM disease and have high predictive value for T2DM.

To explore the clinical characteristics and long-term prognosis of patients with prediabetic cerebral hemorrhage.

One hundred and sixty-five patients with intracerebral hemorrhage were retrospectively analyzed. According to the blood glucose levels, patients were classified as prediabetes group(n=56) and normal glucose metabolism group(n=109). The baseline data and clinical characteristics were compared between two groups. The modified Rankin scale(mRS) was used to evaluate the prognosis at 1 year poststroke.

The poor prognosis of intracerebral hemorrhage was related to age, hematoma volume, ventricle rupture, hematoma enlargement, National Institutes of Health Stroke Scale (NIHSS) score, concurrent infection, C-reactive protein (CRP), fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), etc. Compared with the normal glucose metabolism group, in addition to the increased HbA1c after admission, the baseline data of the prediabetic group showed no statistically significant difference. However, after intracerebral hemorrhage, the prediabetic group had larger hematoma volume, higher NIHSS score, higher incidence of rupturing into ventricle and accompanying infection. The incidence of poor prognosis in the prediabetic group was higher than that in the normal glucose metabolism group (71.43% versus 32.11%, P < 0.05).

Prediabetic patients with cerebral hemorrhage have more severe condition and worse prognosis. Clinical attention should be paid to clinical manifestations in patients with prediabetic cerebral hemorrhage.

To evaluate the correlations of growth arrest specific 6 (GAS6) and C1q/TNF-related protein 4 (CTRP4) levels with carotid atherosclerosis (CAS) in patients with type 2 diabetes (T2DM).

A total of 60 T2DM patients with CAS confirmed by ultrasound were retrospectively selected as T2DM+CAS group, and 60 T2DM patients without CAS confirmed by ultrasound were selected as T2DM group according to a matching ratio of 1 to 1 in gender as well as addition and subtraction of age for 2 years. A questionnaire was developed to collect clinical data of the patients. Plasma GAS6 and serum CTRP4 levels were measured by enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to evaluate the risk factors of CAS in T2DM patients. The receiver operating characteristic (ROC) curve was drawn and the area under the curve (AUC) was calculated to evaluate the predictive efficacy of plasma GAS6 level and serum CTRP4 level for CAS in T2DM patients.

There were no significant differences in age, body mass index, waist circumference, smoking, blood pressure and levels of relevant biochemical indicators between the two groups (P>0.05). The prevalence of hypertension in the T2DM+CAS group was higher than that in the T2DM group, and the levels of GAS6 and CTRP4 were lower than those in the T2DM group, and the differences were statistically significant (P < 0.05). Logistic regression analysis showed that increase of every 1 ng/mL in GAS6 and CTRP4 levels respectively leaded to 0.508 times (OR=0.508, 95%CI, 0.345 to 0.747, P=0.001) and 0.883 times of decrease in the risk of CAS in T2DM patients (OR=0.883, 95%CI, 0.819 to 0.952, P=0.001). Hypertension increased the risk of CAS in T2DM patients by 3.051 times (OR=3.051, 95%CI, 1.438 to 6.473, P=0.004). The maximum Youden index of GAS6 or CTRP4 alone and their combination in predicting CAS in T2DM patients was 0.417, 0.384 and 0.517, respectively, with the corresponding sensitivity of 85.0%, 81.7% and 81.7%, and the specificity of 58.3%, 56.7% and 70.0%, respectively.

Plasma GAS6 and CTRP4 levels are negatively correlated with the risk of CAS in T2DM patients, and the combination of plasma GAS6 and CTRP4 has a good predictive value for CAS in T2DM patients.

To investigate the death-related influencing factors and pathogenic distribution characteristics of type 2 diabetes mellitus (T2DM) patients complicated with severe community-acquired pneumonia (SCAP).

The clinical materials of 202 T2DM patients complicated with SCAP in Zhengzhou Central Hospital Affiliated to Zhengzhou University from January 2020 to December 2021 were retrospectively analyzed, and they were divided into survival group with 147 cases and death group with 55 cases according to clinical outcomes. The clinical materials of patients in both groups were compared; the univariate and multivariate Logistic regression models were used to analyze prognostic risk factors for T2DM complicated with SCAP.

Age, ratios of complicating heart failure and chronic kidney disease, ratio of invasive ventilation, ratio of bloodstream infection, risks of septic shock and multiple organ failure and ratio of high-risk Pneumonia Severity Index (PSI) score in the death group were significantly higher than those in the survival group (P < 0.05). Multivariate Logistic regression analysis showed that age, glycosylated hemoglobin (HbA1c)>8.0%, high-risk PSI score, complicating heart failure and chronic kidney disease, invasive ventilation, bloodstream infection and septic shock were the independent risk factors for death of T2DM patients complicating with SCAP (P < 0.05). The results of pathogen detection in 202 patients showed that the top three pathogens were Acinetobacter baumannii, Klebsiella pneumoniae and Candida; the top three responsible pathogens were Klebsiella pneumoniae, Streptococcus pneumoniae and Escherichia coli; the risk of death in hospital in patients with Klebsiella pneumoniae pneumonia was correlated with bloodstream infection, lobulated lesions complicating with cavity and septic shock (P < 0.05); death in hospital was correled with complicating mixed infection (P < 0.05).

Clinicians should pay attention to the factors such as advanced age, complicated multiple underlying diseases and HbA1c>8.0% in T2DM patients complicating with SCAP. Once there are bloodstream infection, septic shock and invasive ventilation for multiloba pneumonia, it indicates that the condition of disease is critical and fatality rate is relatively high, which requires early identification and intervention.

To explore the effects of nursing model of peer education based on Wechat platform on blood glucose, quality of life and nursing satisfaction of diabetic patients.

The data of 80 patients with type 2 diabetes mellitus were retrospectively analyzed and divided into peer education group(n=42)and traditional nursing group(n=38)according to different nursing modes. The peer education group was given peer education based on Wechat group, and a multi-dimensional, all-round and one-stop predictive nursing model was adopted. The traditional nursing group was treated with traditional nursing mode. Fasting blood glucose, 2 h postprandial blood glucose, glycosylated hemoglobin (HbA1c), quality of life and nursing satisfaction of the two groups were compared 12 months after discharge.

At 12 months after discharge, the levels of fasting blood glucose, 2 h postprandial blood glucose and HbA1c in the peer education nursing group were lower than those in the traditional nursing group (P < 0.001); the score of Quality of Life Questionnaire-short version (WHOQOL-BREF) in the peer education group was significantly higher than that in the traditional nursing group (P < 0.001); the nursing satisfaction of the peer education group was 97.62%, which was higher than 78.95% of the traditional nursing group (P < 0.05).

The nursing model of peer education based on Wechat group can effectively stabilize blood glucose level and improve the long-term self-management ability, thereby improving the quality of life and nursing satisfaction.

To explore the relationships of social support with depression, anxiety and pregnancy outcome in patients with gestational diabetes mellitus (GDM) in the second and third trimester of pregnancy.

A longitudinal study was conducted to investigate the patients diagnosed with GDM in Yangzhou Maternal and Child Health Hospital from July 2020 to March 2022 by using Social Support Rating Scale (SSRS), Self-Rating Depression Scale (SDS), Self-Rating Anxiety Scale (SAS) and other tools, and to follow up the pregnancy outcomes.

The social support scores of 211 GDM patients were (38.39±6.17), which were in the middle level. There were 102 cases of adverse pregnancy outcomes. Linear regression analysis showed that the working situation of pregnant women significantly affected the total score of social support (P < 0.05), family residence and family economic support during pregnancy affected the objective support score (P < 0.05), and the parity affected the subjective support score (P < 0.05). Workingstatus, husband's education background and family economic support during pregnancy affected the utilization of support (P < 0.05). The results of correlation analysis showed that objective support, utilization of support and total social support were negatively correlated with postpartum hemorrhage (r=-0.194, -0.240, -0.203, P < 0.05). Binary Logistic regression analysis showed that the utilization of support was a protective factor for postpartum hemorrhage (OR=0.440; 95%CI, 0.264 to 0.734; P=0.002), and depression (OR=2.118; 95%CI, 1.088 to 4.125; P=0.027) and pre-pregnancy body mass index (OR=1.223; 95%CI, 1.096 to 1.364; P < 0.001) were risk factors for macrosomia in GDM patients, and husband's education background was a protective factor for macrosomia in GDM patients (OR=0.268; 95%CI, 0.120 to 0.595, P=0.001).

The level of social support is closely related to depression and anxiety. Therefore, improving the level of social support in the second and third trimesters of pregnancy is a potential intervention strategy to improve the pregnancy outcome of GDM patients.